A review of the effects of insulin-like growth factor and platelet derived growth factor on in vivo cartilage healing and repair M.B. Schmidt, Ph.D.,

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Presentation transcript:

A review of the effects of insulin-like growth factor and platelet derived growth factor on in vivo cartilage healing and repair M.B. Schmidt, Ph.D., E.H. Chen, Ph.D., S.E. Lynch, D.M.D., D.M.Sc. Osteoarthritis and Cartilage Volume 14, Issue 5, Pages 403-412 (May 2006) DOI: 10.1016/j.joca.2005.10.011 Copyright © 2005 OsteoArthritis Research Society International Terms and Conditions

Fig. 1 Macroscopic appearance of control (A) and IGF-1 treated (B) cartilage defects in an equine model at 6 months post-op. Treated defects were filled with a fibrin clot supplemented with IGF-1 at the time of surgery. Control defects were filled with a fibrin clot alone. IGF treatment improves the quantity and quality of new cartilage filling the defect8. Osteoarthritis and Cartilage 2006 14, 403-412DOI: (10.1016/j.joca.2005.10.011) Copyright © 2005 OsteoArthritis Research Society International Terms and Conditions

Fig. 2 Histological appearance of cell-seeded cartilage defects in an equine model at 8 months post-op. Treated defects were filled with a fibrin clot seeded with chondrocytes and supplemented with IGF-1. Control defects were filled with a similarly seeded clot without the growth factor. Hematoxylin and eosin staining of entire control defect (A) and IGF-1 treated defect (B) demonstrates slightly improved fill in the treated defects. Comparison of toluidine blue staining of control tissue (C) and treated (D) repair tissue shows a significant increase in staining intensity in IGF-1 supplemented repair tissue10 (A, B bar=5mm; C, D bar=80μm). Osteoarthritis and Cartilage 2006 14, 403-412DOI: (10.1016/j.joca.2005.10.011) Copyright © 2005 OsteoArthritis Research Society International Terms and Conditions

Fig. 3 [35S]-Sulfate incorporation of confluent cultures of resting zone chondrocytes demonstrates a dose-dependent response, following treatment with PDGF-BB for 24h. Values are the mean±s.e.m. of six cultures, *P<0.05, treatment vs control20. Osteoarthritis and Cartilage 2006 14, 403-412DOI: (10.1016/j.joca.2005.10.011) Copyright © 2005 OsteoArthritis Research Society International Terms and Conditions

Fig. 4 The effect of PDGF-BB pretreatment of resting zone chondrocytes on heterotopic cartilage formation (*P<0.05, all groups vs implant alone, #P<0.05, 4 vs 8 weeks, ●P<0.05 PDGF-BB treatment for 24 vs 4h)21. Osteoarthritis and Cartilage 2006 14, 403-412DOI: (10.1016/j.joca.2005.10.011) Copyright © 2005 OsteoArthritis Research Society International Terms and Conditions

Fig. 5 Dose–response results for proteoglycan synthesis in meniscal explants exposed to IGF-1 (A) and PDGF-AB (B). Explants were subjected to static compressive loads for 4 days. Data are normalized to basal medium (BSA) control values. Star denotes that the value is significantly different from the BSA controls (P<0.05). Both growth factors stimulated proteoglycan synthesis, with PDGF-AB exhibiting a stronger effect24. Osteoarthritis and Cartilage 2006 14, 403-412DOI: (10.1016/j.joca.2005.10.011) Copyright © 2005 OsteoArthritis Research Society International Terms and Conditions

Fig. 6 Summary of the in vitro and in vivo effects of IGF on cartilage and meniscal repair. Osteoarthritis and Cartilage 2006 14, 403-412DOI: (10.1016/j.joca.2005.10.011) Copyright © 2005 OsteoArthritis Research Society International Terms and Conditions

Fig. 7 Summary of the in vitro and in vivo effects of PDGF on cartilage and meniscal repair. Osteoarthritis and Cartilage 2006 14, 403-412DOI: (10.1016/j.joca.2005.10.011) Copyright © 2005 OsteoArthritis Research Society International Terms and Conditions