Secretory phospholipase A2 is required to produce histologic changes associated with gastroduodenal reflux in a murine model Ashok Babu, MD, Xianzhong.

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Presentation transcript:

Secretory phospholipase A2 is required to produce histologic changes associated with gastroduodenal reflux in a murine model Ashok Babu, MD, Xianzhong Meng, MD, PhD, Anirban M. Banerjee, PhD, Fabia Gamboni-Robertson, PhD, Joseph C. Cleveland, MD, Sagar Damle, MD, David A. Fullerton, MD, Michael J. Weyant, MD The Journal of Thoracic and Cardiovascular Surgery Volume 135, Issue 6, Pages 1220-1227 (June 2008) DOI: 10.1016/j.jtcvs.2007.10.080 Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 Schematic representation of the surgical model of creating mixed gastroduodenal (DGEA) reflux. The Journal of Thoracic and Cardiovascular Surgery 2008 135, 1220-1227DOI: (10.1016/j.jtcvs.2007.10.080) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 Diagram demonstrating the role of secretory phospholipase A2 (sPLA2) in the arachidonic acid pathway. The Journal of Thoracic and Cardiovascular Surgery 2008 135, 1220-1227DOI: (10.1016/j.jtcvs.2007.10.080) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 Immunofluorescence analysis of DGEA esophageal tissue in study animals demonstrating no identifiable protein in C57BL/6 mice and identifiable secretory phospholipase A2 (sPLA2) protein (red staining) in BALB/c mice (A) compared with that seen in negative control animals (B). The Journal of Thoracic and Cardiovascular Surgery 2008 135, 1220-1227DOI: (10.1016/j.jtcvs.2007.10.080) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

Figure 4 Hematoxylin and eosin analysis of mucosal thickness at 20× magnification. Significant increase in mucosal thickness in response to DGEA in BALB/c secretory phospholipase A2 (sPLA2) +/+ mice (n = 5; A) compared with C57BL/6 sPLA2−/− mice (n = 5; B) is shown. The Journal of Thoracic and Cardiovascular Surgery 2008 135, 1220-1227DOI: (10.1016/j.jtcvs.2007.10.080) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

Figure 5 Ki67 immunohistochemistry demonstrating a significant increase in proliferating cells in response to DGEA in BALB/c secretory phospholipase A2 (sPLA2) +/+ mice (n = 5; A) compared with C57BL/6 sPLA2−/− mice (n = 5; B). Black arrowheads point to some of the positive nuclei as an example. HPF, High-powered field. The Journal of Thoracic and Cardiovascular Surgery 2008 135, 1220-1227DOI: (10.1016/j.jtcvs.2007.10.080) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions