Sperm nuclear vacuoles, as assessed by motile sperm organellar morphological examination, are mostly of acrosomal origin O Kacem, C Sifer, V Barraud-Lange,

Slides:

Advertisements

Similar presentations

Clinical benefit using sperm hyaluronic acid binding technique in ICSI cycles: a systematic review and meta-analysis Ronit Beck-Fruchter, Eliezer Shalev,

Advertisements

Programmes and prospects for ovotechnology

Attitudes and policies regarding access to fertility care and assisted reproductive technologies in Israel Daniel Sperling, Yael Simon Reproductive.

Diet and endometriosis risk: A literature review

Recording and sorting live human sperm undergoing acrosome reaction

Sperm macrocephaly syndrome in a patient without AURKC mutations and with a history of recurrent miscarriage Emanuela Molinari, Marzia Mirabelli, Stefania.

In-vitro effects of Thymus munbyanus essential oil and thymol on human sperm motility and function Amirouche Chikhoune, Laurence Stouvenel, Mokrane Iguer-Ouada,

Small human sperm vacuoles observed under high magnification are pocket-like nuclear concavities linked to chromatin condensation failure F. Boitrelle,

Delphine Haouzi, Hervé Dechaud, Said Assou, John De Vos, Samir Hamamah

Effect of vascular endothelial growth factor on sperm motility and survival Ahmet Cem Iyibozkurt, Pelin Balcik, Sibel Bulgurcuoglu, Burcu Kardaş Arslan,

Methodology matters: IVF versus ICSI and embryonic gene expression

E. Jauniaux, I. Ben-Ami, R. Maymon Reproductive BioMedicine Online

Clinical benefit using sperm hyaluronic acid binding technique in ICSI cycles: a systematic review and meta-analysis Ronit Beck-Fruchter, Eliezer Shalev,

An Bracke, Kris Peeters, Usha Punjabi, David Hoogewijs, Sylvia Dewilde

Sperm immobilized before intracytoplasmic sperm injection undergo ultrastructural damage and acrosomal disruption Maria José Gómez-Torres, Ph.D., Jorge.

Multi-centre assessment of nitroblue tetrazolium reactivity in human semen as a potential marker of oxidative stress Jaime Gosálvez, Lamberto Coppola,

E. Jauniaux, I. Ben-Ami, R. Maymon Reproductive BioMedicine Online

Repeated implantation failure at the crossroad between statistics, clinics and over- diagnosis Edgardo Somigliana, Paola Vigano, Andrea Busnelli, Alessio.

Single layer centrifugation of stallion spermatozoa improves sperm quality compared with sperm washing J.M. Morrell, H. Rodriguez-Martinez, A. Johannisson

External quality control for embryology laboratories

‘It's a bit too much fathering this seed’: the meaning-making of the sperm donor in Italian lesbian mother families Vittorio Lingiardi, Nicola Carone,

Correlation between DNA defect and sperm-head morphology

Katja Knez, Tomaz Tomazevic, Eda Vrtacnik-Bokal, Irma Virant-Klun

Sperm attachment and penetration competence in the human oocyte: a possible aetiology of fertilization failure involving the organization of oolemmal.

Why are reproductive cancers more common in nulliparous women?

M.M. Francou, J. Ten, R. Bernabeu, J. De Juan

The effect of a high progesterone concentration before oocyte retrieval on the peri- implantation endometrium Liu Liu, Sumaia Sailan, Tinchiu Li, Najat.

Désirée García, Rita Vassena, Andrés Prat, Valérie Vernaeve

Kiran Singh, Rohini R. Nair, Anuradha Khanna

Development of a real-time PCR method for rapid sexing of human preimplantation embryos CD Martinhago, LD Vagnini, CG Petersen, AL Mauri, RL Baruffi,

Evaluation of sperm nuclear integrity in patients with different percentages of decapitated sperm in ejaculates Christine Rondanino, Véronique Duchesne,

S Munné, E Fragouli, P Colls, MG Katz-Jaffe, WB Schoolcraft, D Wells

Christophe Audouard, M.S., Elisabeth Christians, Ph.D.

Effect of N-acetyl-cysteine after ovarian drilling in clomiphene citrate-resistant PCOS women: a pilot study A. Nasr Reproductive BioMedicine Online

L. Simon, I. Proutski, M. Stevenson, D. Jennings, J. McManus, D

Cumulative live-birth delivery after IVF/ICSI since the progressive introduction of single- embryo transfer D. De Neubourg, C. Daels, M. Elseviers, K.

N. Geerts, J. McGrath, J.N. Stronk, T.K. Vanderlick, G. Huszar

Small human sperm vacuoles observed under high magnification are pocket-like nuclear concavities linked to chromatin condensation failure F. Boitrelle,

Chief editor's 2017 annual report

Effect of energy expenditure and physical activity on the outcomes of assisted reproduction treatment Mert Kucuk, Fadime Doymaz, Bulent Urman Reproductive.

Morphological systems of human embryo assessment and clinical evidence

Easy sperm processing technique allowing exclusive accumulation and later usage of DNA-strandbreak-free spermatozoa T. Ebner, O. Shebl, M. Moser, R.B.

Cross-border reproductive care: a review of the literature

The incidence of long heterochromatic polymorphism variants in infants conceived through assisted reproductive technologies Andrew Wilson, Katherine.

The relationship between sperm head retardance using polarized light microscopy and clinical outcomes Belinda G. Vermey, Michael G. Chapman, Simon Cooke,

Sedimentation properties in density gradients correspond with levels of sperm DNA fragmentation, chromatin compaction and binding affinity to hyaluronic.

Donor information considered important to donors, recipients and offspring: an Australian perspective I.S. Rodino, P.J. Burton, K.A. Sanders Reproductive.

Does being overweight affect seminal variables in fertile men?

Comparison of DNA fragmentation levels in spermatozoa with different sex chromosome complements Xiao Shi, David Yiu Leung Chan, Ming Peng Zhao, Carol.

Vitrification is not superior to rapid freezing of normozoospermic spermatozoa: effects on sperm parameters, DNA fragmentation and hyaluronan binding

Cumulative delivery rates after ICSI with donor spermatozoa in different age groups Michaël De Brucker, Michel Camus, Patrick Haentjens, Jacques Francotte,

Low birth defects by deselecting abnormal spermatozoa before ICSI

Russell–Silver syndrome due to paternal H19/IGF2 hypomethylation in a patient conceived using intracytoplasmic sperm injection M. Chopra, D.J. Amor,

Nuclear volume differences between balanced and unbalanced spermatozoa in chromosomal translocation carriers Alexandre Rouen, Alinoë Lavillaureix, Capucine.

Should we be promoting embryo transfer at blastocyst stage?

Stephen C. Collins, Esther Chan Reproductive BioMedicine Online

Pietro Gambadauro, Ramesan Navaratnarajah

Implication of apoptosis in sperm cryoinjury

Selection of embryos for transfer in IVF: ranking embryos based on their implantation potential using morphological scoring Laura van Loendersloot, Madelon.

The Alpha Consensus Meeting on the professional status of the clinical embryologist: proceedings of an expert meeting Reproductive BioMedicine Online

Response: time-lapse systems for ART

C Almeida, M Sousa, A Barros Reproductive BioMedicine Online

Adenomyosis and infertility

Ovarian reserve screening before contraception?

Adriana M. Caille, Cesar L. Berta, Patricia S

Programmes and prospects for ovotechnology

Claudio Manna, Loris Nanni, Alessandra Lumini, Sebastiana Pappalardo

M. Seiringer, M. Maurer, O. Shebl, K. Dreier, G. Tews, S. Ziehr, G

James D. Stanger, Long Vo, John L. Yovich, Ghanim Almahbobi

China’s model of egg donation is a policy lesson for Britain

Presentation transcript:

Sperm nuclear vacuoles, as assessed by motile sperm organellar morphological examination, are mostly of acrosomal origin O Kacem, C Sifer, V Barraud-Lange, B Ducot, D De Ziegler, C Poirot, JP Wolf Reproductive BioMedicine Online Volume 20, Issue 1, Pages 132-137 (January 2010) DOI: 10.1016/j.rbmo.2009.10.014 Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Acrosome detection on normal spermatozoa, according to sperm organellar morphological examination. Spermatozoa were immobilized and dried on a smear. The smear was then analysed by Normaski interferential contrast (A and B) and for the acrosomal status using the Pisum sativum agglutinin–fluorescein isothiocyanate (PSA-FITC) detection technique and immunofluorescence (A′ and B′). Merge (A″ and B″). Note the absence of fluorescence after PSA-FITC staining (×6600). Reproductive BioMedicine Online 2010 20, 132-137DOI: (10.1016/j.rbmo.2009.10.014) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Acrosome detection on spermatozoa with nuclear vacuoles, according to sperm organellar morphological examination. Spermatozoa with nuclear vacuoles (see Figure 1) (×6600) (A) spermatozoon with incomplete acrosome reaction, (B) acrosome intact. Immunofluorescence (A′ and B′). Merge (A″ and B″). Highlighted green spots can be seen in the location of the vacuoles (arrows). These spermatozoa had a fluorescent zone on all the anterior head suggesting an intact acrosome or incomplete acrosome reaction (×6600). Reproductive BioMedicine Online 2010 20, 132-137DOI: (10.1016/j.rbmo.2009.10.014) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Live motile spermatozoa analysed by motile sperm organellar morphological examination at consecutive time points. The same spermatozoon with a nuclear vacuole is shown on two consecutive pictures. Panels (A) and (B) show a live motile spermatozoon with protruding blebs (arrows) in the acrosomal region. It could be interpreted as an ongoing acrosome reaction. A different spermatozoon in shown panel (C) exhibits an embossed image. However, the same image looks like a defect of material on the same spermatozoon in (D) (arrows) (×6600). Reproductive BioMedicine Online 2010 20, 132-137DOI: (10.1016/j.rbmo.2009.10.014) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions