Estimation of the identity of proteolytic aggrecan fragments using PAGE migration and Western immunoblot A. Struglics, Ph.D., S. Larsson, B.Sc., L.S.

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Estimation of the identity of proteolytic aggrecan fragments using PAGE migration and Western immunoblot A. Struglics, Ph.D., S. Larsson, B.Sc., L.S. Lohmander, M.D., Ph.D. Osteoarthritis and Cartilage Volume 14, Issue 9, Pages 898-905 (September 2006) DOI: 10.1016/j.joca.2006.02.016 Copyright © 2006 OsteoArthritis Research Society International Terms and Conditions

Fig. 1 Human aggrecan fragments in SF (A) and in cartilage (B and C) samples were detected by Western immunoblot. The samples were separated by SDS-PAGE and after transfer to PVDF-membranes probed with different antibodies as shown. Different amount of GAG per lane and different film exposure times were used to facilitate comparisons between the immunobands. Immuno-detected aggrecan fragments having only one identified terminal end are marked by their Mw values (in kDa), while fragments marked by amino acids (or marked by a domain) have verified N- and C-terminal ends. The fragment marked # (B) has been identified as the deglycosylation enzyme chondroitinase ABC (results not shown). SF, synovial fluid D1-fraction; C, cartilage A1D1-fraction; C-MMP, MMP-3 in vitro digested cartilage. The positions of Mw markers are indicated (in kDa) in the figures. Osteoarthritis and Cartilage 2006 14, 898-905DOI: (10.1016/j.joca.2006.02.016) Copyright © 2006 OsteoArthritis Research Society International Terms and Conditions

Fig. 1 Human aggrecan fragments in SF (A) and in cartilage (B and C) samples were detected by Western immunoblot. The samples were separated by SDS-PAGE and after transfer to PVDF-membranes probed with different antibodies as shown. Different amount of GAG per lane and different film exposure times were used to facilitate comparisons between the immunobands. Immuno-detected aggrecan fragments having only one identified terminal end are marked by their Mw values (in kDa), while fragments marked by amino acids (or marked by a domain) have verified N- and C-terminal ends. The fragment marked # (B) has been identified as the deglycosylation enzyme chondroitinase ABC (results not shown). SF, synovial fluid D1-fraction; C, cartilage A1D1-fraction; C-MMP, MMP-3 in vitro digested cartilage. The positions of Mw markers are indicated (in kDa) in the figures. Osteoarthritis and Cartilage 2006 14, 898-905DOI: (10.1016/j.joca.2006.02.016) Copyright © 2006 OsteoArthritis Research Society International Terms and Conditions

Fig. 1 Human aggrecan fragments in SF (A) and in cartilage (B and C) samples were detected by Western immunoblot. The samples were separated by SDS-PAGE and after transfer to PVDF-membranes probed with different antibodies as shown. Different amount of GAG per lane and different film exposure times were used to facilitate comparisons between the immunobands. Immuno-detected aggrecan fragments having only one identified terminal end are marked by their Mw values (in kDa), while fragments marked by amino acids (or marked by a domain) have verified N- and C-terminal ends. The fragment marked # (B) has been identified as the deglycosylation enzyme chondroitinase ABC (results not shown). SF, synovial fluid D1-fraction; C, cartilage A1D1-fraction; C-MMP, MMP-3 in vitro digested cartilage. The positions of Mw markers are indicated (in kDa) in the figures. Osteoarthritis and Cartilage 2006 14, 898-905DOI: (10.1016/j.joca.2006.02.016) Copyright © 2006 OsteoArthritis Research Society International Terms and Conditions