Warm water Hot Water Cold Water (Fast Reaction) (Moderate reaction) (slow reaction)

12/13 Warm up: What can you say about the reaction rates of the pepsin and trypsin enzymes and pH? Pepsin works better at pH2 Trypsin works better at pH8

Spiders and Enzymes To feed, black widows puncture their insect prey with their fangs and administer digestive enzymes to the corpses. By using these enzymes, and their gnashing fangs, the spiders liquefy their prey's bodies and suck up the resulting fluid.

Silence Please be courteous, And put away Detention! Thanks! And avoid Your mobile devices in class And avoid Detention! Thanks!

12/13 Bell Work: what do you think was in liver, potato and mushroom that reacted with Hydrogen peroxide? + = Reaction! We think it might be another chemical, actually is an enzyme called catalase ?

Why does our bodies produce hydrogen Peroxide? When the immune system is activated in response to bacteria, large amounts of hydrogen peroxide are produced by certain cells to fight the infection. But high levels of H2O2 can damage the cells. Therefore the liver breaks down H2O2 with the help of Catalase https://www.youtube.com/watch?v=bJhSy_HmkUE

2H2O2 Catalase (Liquid) ? + (gas) ? One of the products of this reaction is a gas, the other is a clear liquid. Looking at the elements present in hydrogen peroxide (H2O2), what might be the clear liquid formed? Based on the tests performed in class, what could the gas be? Complete the following chemical equation (be sure you balance it!):

https://www.youtube.com/watch?v=bJhSy_HmkUE https://www.youtube.com/watch?v=qgVFkRn8f10 enzymes https://www.youtube.com/watch?v=wbh3SjzydnQ liver

Enzymes Notes Enzymes are proteins that assist (help) biochemical reactions by bringing key molecules together. Enzymes are called catalysers(they speed reactions). http://programs.northlandcollege.edu/biology/Biology1111/animations/enzyme.html

Ex: Milk and Lactase The main cause of lactose intolerance is the enzyme lactase lack. The enzyme lactase is known to break down lactose into galactose and glucose. If the body is unable to digest lactose contained in milk, the person suffers from lactose intolerance.

What are enzymes important? They help cells regulate the cell cycle...reactions work faster and less energy is used!

Enzyme Structure Enzymes have at least one active site only one certain molecule can fit the active site (lock and key)

Degradation reactions (breaking apart) animation

Degradation reactions steps A substrate attaches to an enzyme’s active site. Enzyme flexes to conform to the substrate’s shape and breaks bonds on substrate. Enzyme is not changed but new products are formed.

Ex: Catalase in the liver The enzyme catalase breaks down the waste substance hydrogen peroxide into water and oxygen. catalase Hydrogen peroxide oxygen + water (enzyme) (substrate) (products)

Synthesis Reaction (putting together) Active site

Plants use enzymes to change glucose into starch Synthesis reaction Glucose-1-phosphate Starch Plants use enzymes to change glucose into starch

Enzyme activity Temperature and pH affect the activity of an enzyme.

Optimum Condition Enzymes function best or are most active in specific conditions known as optimum conditions. Enzymes might become denatured and unusable at higher temp. or pH

You can find enzymes in… in toothpaste laundry detergent meat tenderizers chocolate covered cherries your cells!

Silence Please be courteous, And put away Detention! Thanks! And avoid Your mobile devices in class And avoid Detention! Thanks!

12/14 bell work: what is the optimum temperature for this Catalase enzyme? (HINT: Where is the rate the highest?) At ~ 43°C

Enzyme Cut –Outs Activity Instructions

Silence Please be courteous, And put away Detention! Thanks! And avoid Your mobile devices in class And avoid Detention! Thanks!

12/18: Bell Work: Is this a Degradation (breaking apart) or synthesis (putting together) enzyme reaction? Label the parts A thru D This is degradation (breaking apart) A= substrate B= Enzyme C=Substrate/ Enzyme complex D= enzyme E= products

Enzyme + substrate → enzyme-substrate complex → enzyme + product 1 + product 2

Lock and Key http://www.lewport.wnyric.org/jwanamaker/animations/Enzyme%20activity.html