Jamming and no nanosilos in pure DPPC:POPG 7:3 25mN/m 30mN/m -At higher pressures, there is a loss of disordered fluid phase (lower height) -FM bright phase associated with “fluid” region is as tightly packed as the condensed phase at higher pressure -At 70mN/m, monolayer is solid (‘jammed’) 60mN/m 70mN/m

Nanosilos and de-jamming of DPPC:POPG 7:3 w/ peptide SPB125 SPB925 AFM depositions done at 25C on a 3.18mPa·sec subphase at ∏=60mN/m Nanosilo size distributions: SPB125 SPB925 radius height 3, 9, 14nm 4nm 50-150nm 100nm even at high pressures (60mN/m) LC domains remain separated by disordered granular phase with all four peptides disordered granular phase allows for changes in S(q) and the new de-jamming stress relaxation pathway A LC domains: equal height (brighter, uniform) [dark phase on FM] disordered phase: granular with LC islands and lower (1nm) fluid phase coexisting with nanosilo stacks [bright phase on FM]

Nanosilos and de-jamming of DPPC:POPG 7:3 w/ peptide SPB125Nflex SPB1125 Nanosilo size distributions: 125Nflex SPB1125 radius height 2-3nm 3nm 35-40nm 50nm nanosilos also smeared on imaging indicating they were less stable tryptophan 9 plays a role in nanosilo stability the N-terminus clearly has some structural specificity due to the poly-P sequence that greatly enhances peptide mediated nanosilo formation nanosilo size: SPB125 >> SPB925, 125Nflex, 1125

Glycerol using glycerol/water solutions the subphase viscosity is modulated between 0.89 and 114 mPa•sec the most striking distinction in monolayer behavior between the different subphases subphases is mode of collapse at near zero surface tensions. system: DPPC:POPG 7:3 monolayer at 25oC under Argon compressed at 0.1mm/sec 0% glycerol (0.89 mPa•sec) 10% glycerol (1.15 mPa•sec) http://lipid.uchicago.edu/~lukap/Haim/glycerol_series/ get movie called DV_0%.mov http://lipid.uchicago.edu/~lukap/Haim/glycerol_series/ get movie called DV_10%.mov

system: DPPC:GM1 ? monolayer at 30oC compressed at 0.1mm/sec