Genomic alterations in breast cancer cell line MDA-MB-231.

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Genomic alterations in breast cancer cell line MDA-MB-231. Genomic alterations in breast cancer cell line MDA-MB-231. A–C, representative genome images from the Integrated Genome Viewer (IGV) for several alterations found in the breast cancer cell line MDA-MB-231. The number of reads for the reference allele and the variant allele are shown for each alteration. A, BRAF oncogene point mutation. B, point mutation in the TP53 tumor suppressor gene. C, a 1-bp insertion in tumor suppressor NF1. D, sequence coverage for each target exon in breast cancer cell line MDA-MB-231 compared with a normal diploid sample. Targets from several genes with copy-number gains and losses are highlighted. E, comparison of gene-level copy-number alterations as detected by exon capture and copy-number data previously obtained with a high-density single-nucleotide polymorphism (SNP) array (Affymetrix SNP 6.0 platform). Several genes with copy-number gains and losses are highlighted. Copy-number data are highly correlated, with a correlation coefficient of 0.94. Nikhil Wagle et al. Cancer Discov 2012;2:82-93 ©2012 by American Association for Cancer Research