Suppl.Figure 1 Transfected HEK293 cells -130kDa -100KDa Blot: -V5

Slides:

Advertisements

Similar presentations

Supplementary File 1: Target areas of each of the CYP2D siRNAs. Homologous areas of each of the CYP2D isoforms to each of the siRNAs were determined by.

Advertisements

ß-gal NME2 NME1 Actin NME1 NME2 Scrambled RISC Free Supplementary data Figure 1B. Antibody L-15 is specific for NME2 and sc-465 is specific for NME1. Western.

Adipose PancreasAorta MuscleHeart Liver LungKidney Testis Spleen Brian 18S p55 γ p55 α Figure S1 Tissue distribution of p55 . (A) Representative RT-PCR.

RASSF2DSARAH-GF (b) MST1-F (a) M F G 70 kD a 55 kD b 36 kD

1&3: hGH-sc(Fc)2 2&4: hGH-Fc anti-Fc anti-hGH

supplementary Fig. 1 Anti-HSP70 Ab Anti-SHP2 Ab

Invest. Ophthalmol. Vis. Sci ;49(7): doi: /iovs Figure Legend:

Figure 3. CSE increases the production of IL-8 in A549 cells

Invest. Ophthalmol. Vis. Sci ;48(1):1-10. doi: /iovs Figure Legend:

High Expression of Sphingosine 1-Phosphate Receptors, S1P1 and S1P3, Sphingosine Kinase 1, and Extracellular Signal-Regulated Kinase-1/2 Is Associated.

From: Lentiviral Vector-Mediated PAX6 Overexpression Promotes Growth and Inhibits Apoptosis of Human Retinoblastoma Cells Invest. Ophthalmol. Vis. Sci..

Supplementary Figure 1: Clinical parameters of the breast tumor samples used for the study. IDC = Invasive ductal carcinoma and ILC= Invasive Lobular.

From: Mitochondrial Localization and Ocular Expression of Mutant Opa3 in a Mouse Model of 3-Methylglutaconicaciduria Type III Invest. Ophthalmol. Vis.

A B C MDA-MB-231 OHT: h FOXM1 PLK CDC25b ERα Tubulin

H3K9me2 and G9a levels decrease during adipogenesis.

A549 cells were transfected with the indicated plasmids

Analysis of CD151 and CD147 as potential prostate cancer biomarkers.

NG2-CreERT2 postnatal day 31

Membrane‐delocalized eIF4G is not able to promote translation.

The gap junction protein connexin43 interacts with the second PDZ domain of the zona occludens-1 protein Ben N.G. Giepmans, Wouter H. Moolenaar Current.

Supplement Figure 3 SYF cells

Figure 2 M1 and M23 AQP4 isoforms compared by freeze-fracture electron microscopic and Western blot analyses M1 and M23 AQP4 isoforms compared by freeze-fracture.

Production of recombinant KRBV NS1 and antiserum reactivity.

Figure e-1 A B C Wild-type TDP-43 G3BP Merge+DAPI

Supplement Figure 2 HEK 293 cells

* * Supplementary figure S4 A B C D

Supplementary Information 1

Supplementary Figure 1. A

Volume 19, Issue 12, Pages (June 2017)

Mara Schvarzstein, Andrew M. Spence Developmental Cell

Andrew W Snowden, Philip D Gregory, Casey C Case, Carl O Pabo

The PbGAPDH G6 fragment interacts with recombinant CD68.

Expression of SYCE2 inhibits the interaction of HP1α with H3K9me3.

Hironori Hara et al. BTS 2018;3:

Adamtsl2 targeting strategy and validation of inactivation.

RLE-1, an E3 Ubiquitin Ligase, Regulates C

Supplemental Figure 4 A no agonist MALP2 PAM3CSK4 GFP WT F217S

pY-Tie2 (IP with anti-Tie2) Tie2 P-RET RET P-KIT KIT GAPDH P-AXL AXL

Figure S3. Zhang et al. A B Myc-SIRT1 - + sir2a+/+ sir2a-/-

TRADD is recruited to the TLR4 signaling complex upon LPS stimulation.

HIPK2 decreases the stability of Notch1-IC through proteasome-dependent degradation. HIPK2 decreases the stability of Notch1-IC through proteasome-dependent.

Identification of Transglutaminase 3 Splicing Isoforms

C-terminal JM-B of EGFR is required for EGF-induced recruitment of TRAF4 to EGFR. (A) The HSQC spectra of 100 μM 15N-labeled TRAF4-TRAF in the absence.

TGF-β stimulates SMAD7 expression via FOXO3.

IgG Autoantibodies from Bullous Pemphigoid Patients Recognize Multiple Antigenic Reactive Sites Located Predominantly Within the B and C Subdomains of.

Characterisation of eSRS21 protein using an anti-eSRS21 antibody.

Fig. 7. Ror-GFP binds to Myc-tagged Wnts and Wnt receptors.

Figure 4 DNM1 mutations affect protein levels and self-dimerization (A) HeLa cells were transfected with green fluorescent protein (GFP)-tagged mutant.

Loss of Novel mda-7 Splice Variant (mda-7s) Expression is Associated with Metastatic Melanoma1 Matthew Allen, Barbara Pratscher, Florian Roka, Clemens.

Validation of DNMT3A splice variant expression.

(fold of TGF-β1 response)

Volume 60, Issue 6, Pages (December 2001)

V-2A-GFP/V-2A-coexpressing Mycobacterium tuberculosis antigens and GFP

Fig. 4. Tetracycline-regulated expression of ClC-5 in the HEK293 cells stably expressing gastric H+,K+-ATPase.(A) Alignments of rat ClC-5, human ClC-5,

Expression of IP3R subtypes in HEK cells.

Vps36 interacts with Smo in the absence of Hh

Mutant MFN2 proteins have normal half-lives and are properly localized to mitochondria. Mutant MFN2 proteins have normal half-lives and are properly localized.

Western blotting for GluR1.

co-IP of LEDGF/p75 and MeCP2.

Variation in dUTPase expression in cell lines.

Fig. 5 NDR2 enhances TRIM25-mediated RIG-I K63-linked ubiquitination via facilitating recruitment of TRIM25 to RIG-I. NDR2 enhances TRIM25-mediated RIG-I.

SPDO isoforms are expressed differentially during development and in adult tissues. SPDO isoforms are expressed differentially during development and in.

Fig. 3 C9ORF72 interacts with SMCR8 in a DENN domain–dependent manner.

Specificity of the WDR81 antibody for the antigen.

Western blot analysis for bFGF expression in human glioma cell lines.

Fig. 6 The C9ORF72/SMCR8 complex regulates ULK1.

The predicted new isoform of PCP2 is expressed in retinal ON bipolar cells. The predicted new isoform of PCP2 is expressed in retinal ON bipolar cells.

KDM4A levels affect the distribution of translation initiation factors

NSG2 interacts with AMPAR subunits GluA1 and GluA2.

Fig. 2 hTERT induces expression of heat shock protein genes through HSF1 and interacts with Hsp70-1. hTERT induces expression of heat shock protein genes.

Presentation transcript:

Suppl.Figure 1 Transfected HEK293 cells -130kDa -100KDa Blot: -V5 BANK1-R61 BANK1-H61 Δ2 BANK1 -130kDa -100KDa Blot: -V5 Supplementary Figure 1. Western blot showing three independent transfections of the three major isoform of BANK1. Western blot was performed from lysates of transfected HEK293 cells with BANK1 isoforms tagged at the C-terminus with V5 epitope and GFP. The blot was interrogated with mouse anti-V5 (1:2000). The variations in protein expression from the same isoform are attributed to difference in transfection efficiency There was not observed any differences in degradation products or translational efficiency between the three isoforms. Supplementary Figure 1. Western blot showing three independent transfections of the three major isoform of BANK1. Western blot was performed from lysates of HEK293 transfected cells with BANK1 isoforms tagged at the C-terminus with V5 epitope and GFP. The blot was interrogated with mouse anti-V5 (1:2000). The variations in protein expression from the same isoform are attributed to difference in transfeccion efficiency There was not observed any differences in degradation products or translational efficiency between the three isoforms.