O-linked N-acetylglucosamine (O-GlcNAc) protein modification is increased in the cartilage of patients with knee osteoarthritis L. Tardio, J. Andrés-Bergós,

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General Information Osteoarthritis and Cartilage

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O-linked N-acetylglucosamine (O-GlcNAc) protein modification is increased in the cartilage of patients with knee osteoarthritis L. Tardio, J. Andrés-Bergós, N.E. Zachara, A. Larrañaga-Vera, C. Rodriguez-Villar, G. Herrero-Beaumont, R. Largo Osteoarthritis and Cartilage Volume 22, Issue 2, Pages 259-263 (February 2014) DOI: 10.1016/j.joca.2013.12.001 Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 O-GlcNAcylated protein levels and OGT and OGA presence in human healthy (white dots) and OA (black dots) knee cartilage. (A) Representative western blot and densitometric analysis for O-GlcNAcylated protein measurement using RL2 antibody. (B) Representative western blot and densitometric analysis for the three OGT isoforms, nucleo-cytoplasmic (ncOGT), mitochondrial (mOGT) and small (sOGT); and the two OGA isoforms, long (OGA-L) and short (OGA-S). EZ blue staining was used as protein loading controls. Results are expressed as fold induction for each patient, and the mean ± 95% C.I. n = 10 healthy and n = 12 OA subjects; P values calculated by Mann–Whitney test. Osteoarthritis and Cartilage 2014 22, 259-263DOI: (10.1016/j.joca.2013.12.001) Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 (A) O-GlcNAcylated protein levels and (B) OGT and OGA presence in HOC stimulated with IL-1α for different periods up to 24 h. Representative western blot and densitometric analysis for O-GlcNAcylated protein detection using RL2 antibody the three OGT isoforms, nucleo-cytoplasmic (ncOGT), mitochondrial (mOGT) and small (sOGT), and the two OGA isoforms, long (OGA-L) and short (OGA-S). Results are shown as fold change vs 0 h ± 95% C.I. of four independent experiments. P values calculated by Mann–Whitney test vs Basal. Osteoarthritis and Cartilage 2014 22, 259-263DOI: (10.1016/j.joca.2013.12.001) Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions