Cryopreservation of ovarian tissue for a decade in Denmark: a view of the technique Mikkel Rosendahl, Kirsten Tryde Schmidt, Erik Ernst, Per Emil Rasmussen,

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Cryopreservation of ovarian tissue for a decade in Denmark: a view of the technique Mikkel Rosendahl, Kirsten Tryde Schmidt, Erik Ernst, Per Emil Rasmussen, Anne Loft, Anne Grethe Byskov, Anders Nyboe Andersen, Claus Yding Andersen Reproductive BioMedicine Online Volume 22, Issue 2, Pages 162-171 (February 2011) DOI: 10.1016/j.rbmo.2010.10.015 Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 (A) Two pieces of frozen–thawed human ovarian cortex (5×5mm) transplanted under the skin of an oophorectomized mouse. After 2weeks, macroscopically visible revascularization was established. (B) Human ovarian cortex kept on ice for 20h prior to freezing. Thawed tissue was transplanted under the skin of an oophorectomized mouse for 4weeks. Histology showed healthy primordial follicles surrounded by small blood vessels (arrows). Insert shows histology of the fresh sample. Bars, 100μm and 0.5mm (insert). Reproductive BioMedicine Online 2011 22, 162-171DOI: (10.1016/j.rbmo.2010.10.015) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 The number of patients who had ovarian tissue cryopreserved per year (up to and including November 2009). White bar=Rigshospitalet; light grey bar=Aarhus University Hospital Skejby (transport); black bar=Odense University Hospital (transport); dark grey bar=total (n=405; 386 clinical and 19 donors). Reproductive BioMedicine Online 2011 22, 162-171DOI: (10.1016/j.rbmo.2010.10.015) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Distribution of diagnoses in 386 patients who had ovarian tissue cryopreserved in the clinical programme. N-HL=non-Hodgkin’s lymphoma. Reproductive BioMedicine Online 2011 22, 162-171DOI: (10.1016/j.rbmo.2010.10.015) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 4 FSH concentrations (mean±SEM) in 12 women after autotransplantation of cryopreserved ovarian tissue. Reproductive BioMedicine Online 2011 22, 162-171DOI: (10.1016/j.rbmo.2010.10.015) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions