PCR detection of Burkholderia cepacia complex as one of key factors to handle a long- term outbreak  Klara Dedeckova, Libor Fila, Veronika Skalicka, Jana Bartosova, Tereza Kucerova, Vera Vavrova, Dana Zemkova, Lucie Kalferstova, Oto Melter, Ondrej Cinek, Pavel Drevinek  Journal of Cystic Fibrosis  Volume 11, Issue 5, Pages 440-445 (September 2012) DOI: 10.1016/j.jcf.2012.04.005 Copyright © 2012 European Cystic Fibrosis Society. Terms and Conditions

Fig. 1 Overview of longitudinal microbiological results in subjects with Bcc infection diagnosed by PCR. (A) Cases with subsequent Bcc positive cultures; (B) Cases with negative cultures. All investigations performed within a three-year period that followed the first PCR finding are depicted on a time scale. Sample positivity is indicated by filling appropriate parts of the circle as follows: bottom=PCR examination; top=culture examination. Journal of Cystic Fibrosis 2012 11, 440-445DOI: (10.1016/j.jcf.2012.04.005) Copyright © 2012 European Cystic Fibrosis Society. Terms and Conditions

Fig. 2 Summary of Bcc infections in the Prague CF Centre over 1994–2010. The left bar indicates the number of new cases of the Bcc infection; the right bar represents all cases per calendar year. Patients with ST32 infection are highlighted in black; infections with all other Bcc strains are in gray. Please note that no incidence data is provided for initial two years of the study (1994–1995) during which screening of the CF population on Bcc infection has been commenced. Journal of Cystic Fibrosis 2012 11, 440-445DOI: (10.1016/j.jcf.2012.04.005) Copyright © 2012 European Cystic Fibrosis Society. Terms and Conditions