The Investigator® ESSplex Plus Kit: Fast, sensitive, and robust amplification of the European Standard Set of loci Mario Scherer, Daniel Müller, Sebastian.

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The Investigator® ESSplex Plus Kit: Fast, sensitive, and robust amplification of the European Standard Set of loci Mario Scherer, Daniel Müller, Sebastian Begemann, Britta Steeger, Sarah Pakulla, Melanie Breitbach, Stefan Cornelius, Lars Bochmann, Anke Prochnow, Thomas Schnibbe, Holger Engel Forensic Science International: Genetics Supplement Series Volume 3, Issue 1, Pages e407-e408 (December 2011) DOI: 10.1016/j.fsigss.2011.09.065 Copyright © 2011 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Sensitivity study of the Investigator ESSplex Plus Kit. Different concentrations of Control DNA XY5 were used as a template. Samples were amplified on an ABI GeneAmp PCR System 9700 Thermal Cycler with gold-plated silver block. Analysis was performed on an AB Genetic Analyzer 3500, equipped with a 36cm 8-capillary array and POP-4™ polymer. NTC: no-template control. Note that the y-axis scales were adjusted for best fit. Each sample was run in triplicates, representative data are shown. Forensic Science International: Genetics Supplement Series 2011 3, e407-e408DOI: (10.1016/j.fsigss.2011.09.065) Copyright © 2011 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 Overview of the Investigator ESSplex Plus inhibitor resistance. The assay was tested with six inhibitors (humic acid, hematin, tannic acid, indigo carmine, calcium, and collagen). 500pg of Control DNA XY5 was used as template and PCR was performed under standard conditions in duplicates. 100 RFU was used as the threshold for allele calling. The colour scheme indicates the number of called versus expected alleles. Forensic Science International: Genetics Supplement Series 2011 3, e407-e408DOI: (10.1016/j.fsigss.2011.09.065) Copyright © 2011 Elsevier Ireland Ltd Terms and Conditions