Volume 56, Issue 1, Pages 105-112 (July 2009) Overexpression of ELAV-like Protein HuR is Associated with Increased COX-2 Expression in Atrophy, High-grade Prostatic Intraepithelial Neoplasia, and Incidental Prostate Cancer in Cystoprostatectomies  Francesca Barbisan, Roberta Mazzucchelli, Alfredo Santinelli, Antonio Lopez-Beltran, Liang Cheng, Marina Scarpelli, Francesco Montorsi, Rodolfo Montironi  European Urology  Volume 56, Issue 1, Pages 105-112 (July 2009) DOI: 10.1016/j.eururo.2008.04.043 Copyright © 2008 European Association of Urology Terms and Conditions

Fig. 1 (A–D) HuR staining in normal looking prostate epithelium (A), atrophy (B), high-grade prostatic intraepithelial neoplasia adjacent to normal epithelium (C), and prostatic adenocarcinoma (D). Staining is also present in the nuclei of the stromal cells. (E–H) COX-2 staining in normal looking prostate epithelium (E), atrophy (F), high-grade prostatic intraepithelial neoplasia (G), and prostatic adenocarcinoma (H). European Urology 2009 56, 105-112DOI: (10.1016/j.eururo.2008.04.043) Copyright © 2008 European Association of Urology Terms and Conditions

Fig. 2 HuR staining in the cytoplasm of the secretory cells in normal looking epithelium (NEp), (remote or away from and adjacent to PCa), atrophy (remote or away from and adjacent to PCa), high-grade prostatic intraepithelial neoplasia (HGPIN) (remote or away from and adjacent to PCa), and prostatic adenocarcinoma (PCa) in the cystoprostatectomy (CyP) and radical prostatectomy (RP) specimens. There is an increase in the percentage of cells with cytoplasmic staining from NEp to atrophy, HGPIN, and PCa. (The columns represent the mean; the standard error is also shown.) European Urology 2009 56, 105-112DOI: (10.1016/j.eururo.2008.04.043) Copyright © 2008 European Association of Urology Terms and Conditions

Fig. 3 HuR staining of strong intensity in the cytoplasm of the secretory cells. The values increase progressively from atrophy away from PCa towards PCa. Normal looking epithelium (NEp) shows cells with strong intensity neither remote from nor adjacent to PCa. (The columns represent the mean; the standard error is also shown.) European Urology 2009 56, 105-112DOI: (10.1016/j.eururo.2008.04.043) Copyright © 2008 European Association of Urology Terms and Conditions

Fig. 4 HuR staining in the nuclei of the secretory cells. There is a trend towards a reduced nuclear expression in atrophy, HGPIN, and PCa, compared to NEp. (The columns represent the mean; the standard error is also shown.) European Urology 2009 56, 105-112DOI: (10.1016/j.eururo.2008.04.043) Copyright © 2008 European Association of Urology Terms and Conditions

Fig. 5 COX-2 staining in the cytoplasm of the basal cells. There is reduction in the proportion of positive cells from NEp to atrophy and HGPIN. (The columns represent the mean; the standard error is also shown.) European Urology 2009 56, 105-112DOI: (10.1016/j.eururo.2008.04.043) Copyright © 2008 European Association of Urology Terms and Conditions

Fig. 6 COX-2 staining in the cytoplasm of the secretory cells. There is increase in the percentage of secretory cells with cytoplasmic staining from NEp to atrophy, HGPIN, and PCa. (The columns represent the mean; the standard error is also shown.) European Urology 2009 56, 105-112DOI: (10.1016/j.eururo.2008.04.043) Copyright © 2008 European Association of Urology Terms and Conditions

Fig. 7 COX-2 staining of strong intensity in the cytoplasm of the secretory cells. The values increase from atrophy away from PCa towards PCa. Normal looking epithelium (NEp) shows cells with strong intensity neither remote from nor adjacent to PCa. (The columns represent the mean; the standard error is also shown.) European Urology 2009 56, 105-112DOI: (10.1016/j.eururo.2008.04.043) Copyright © 2008 European Association of Urology Terms and Conditions