Sheila G. Couto, M. Cristina Nonato, Antonio J. Costa-Filho

Slides:

Advertisements

Similar presentations

Creep Function of a Single Living Cell Nicolas Desprat, Alain Richert, Jacqueline Simeon, Atef Asnacios Biophysical Journal Volume 88, Issue 3, Pages

Advertisements

Domain Registration in Raft-Mimicking Lipid Mixtures Studied Using Polymer-Tethered Lipid Bilayers Sumit Garg, Jürgen Rühe, Karin Lüdtke, Rainer Jordan,

Mesoscale Simulation of Blood Flow in Small Vessels Prosenjit Bagchi Biophysical Journal Volume 92, Issue 6, Pages (March 2007) DOI: /biophysj

Determination of the Orientation and Dynamics of Ergosterol in Model Membranes Using Uniform 13C Labeling and Dynamically Averaged 13C Chemical Shift Anisotropies.

Probing the Role of Dynamics in Hydride Transfer Catalyzed by Lactate Dehydrogenase Nickolay Zhadin, Miriam Gulotta, Robert Callender Biophysical Journal.

Different Effects of Trifluoroethanol and Glycerol on the Stability of Tropomyosin Helices and the Head-to-Tail Complex Fernando Corrêa, Chuck S. Farah.

Date of download: 7/10/2016 Copyright © ASME. All rights reserved. From: Three-Dimensional Modeling of Supine Human and Transport System Under Whole-Body.

Dynamic Molecular Structure of DPPC-DLPC-Cholesterol Ternary Lipid System by Spin- Label Electron Spin Resonance Yun-Wei Chiang, Yuhei Shimoyama, Gerald.

Volume 95, Issue 12, Pages (December 2008)

Voltage-Dependent Hydration and Conduction Properties of the Hydrophobic Pore of the Mechanosensitive Channel of Small Conductance Steven A. Spronk,

Maryam Sayadi, Seiichiro Tanizaki, Michael Feig Biophysical Journal

Interaction of LL-37 with Model Membrane Systems of Different Complexity: Influence of the Lipid Matrix E. Sevcsik, G. Pabst, W. Richter, S. Danner,

Vishwanath Jogini, Benoît Roux Biophysical Journal

Toshiro Oda, Keiichi Namba, Yuichiro Maéda Biophysical Journal

Structure and Dynamics of the Membrane-Bound Form of Pf1 Coat Protein: Implications of Structural Rearrangement for Virus Assembly Sang Ho Park, Francesca.

Anionic Lipids Modulate the Activity of the Aquaglyceroporin GlpF

Volume 90, Issue 1, Pages (January 2006)

Rainer A. Böckmann, Amedeo Caflisch Biophysical Journal

Composition Fluctuations in Lipid Bilayers

Photochemical Reaction Dynamics of the Primary Event of Vision Studied by Means of a Hybrid Molecular Simulation Shigehiko Hayashi, Emad Tajkhorshid,

Volume 95, Issue 12, Pages (December 2008)

Volume 90, Issue 1, Pages (January 2006)

Membrane Insertion and Lipid-Protein Interactions of Bovine Seminal Plasma Protein PDC-109 Investigated by Spin-Label Electron Spin Resonance Spectroscopy

Shuichi Toraya, Katsuyuki Nishimura, Akira Naito Biophysical Journal

Volume 106, Issue 6, Pages (March 2014)

Volume 112, Issue 4, Pages (February 2017)

Tracking Phospholipid Populations in Polymorphism by Sideband Analyses of 31P Magic Angle Spinning NMR Liam Moran, Nathan Janes Biophysical Journal

Volume 102, Issue 3, Pages (February 2012)

Volume 107, Issue 6, Pages (September 2014)

EPR Spectroscopy Targets Structural Changes in the E

Experimental and Computational Studies Investigating Trehalose Protection of HepG2 Cells from Palmitate-Induced Toxicity Sukit Leekumjorn, Yifei Wu,

Simulation Studies of Protein-Induced Bilayer Deformations, and Lipid-Induced Protein Tilting, on a Mesoscopic Model for Lipid Bilayers with Embedded.

Coarse-Grained Molecular Dynamics Simulations of Phase Transitions in Mixed Lipid Systems Containing LPA, DOPA, and DOPE Lipids Eric R. May, Dmitry I.

Exterior Site Occupancy Infers Chloride-Induced Proton Gating in a Prokaryotic Homolog of the ClC Chloride Channel David L. Bostick, Max L. Berkowitz

A Second Look at Mini-Protein Stability: Analysis of FSD-1 Using Circular Dichroism, Differential Scanning Calorimetry, and Simulations Jianwen A. Feng,

Volume 90, Issue 1, Pages (January 2006)

Calculating the Free Energy of Association of Transmembrane Helices

Nucleotide Effects on the Structure and Dynamics of Actin

Orsolya Toke, W. Lee Maloy, Sung Joon Kim, Jack Blazyk, Jacob Schaefer

Lipid Headgroups Modulate Membrane Insertion of pHLIP Peptide

Phospholipid-Based Artificial Viruses Assembled by Multivalent Cations

G. Garbès Putzel, Mark J. Uline, Igal Szleifer, M. Schick

Volume 103, Issue 8, Pages (October 2012)

Comparative Molecular Dynamics Simulation Studies of Protegrin-1 Monomer and Dimer in Two Different Lipid Bilayers Huan Rui, Jinhyuk Lee, Wonpil Im

Mesoscopic Modeling of Bacterial Flagellar Microhydrodynamics

Firdaus Samsudin, Alister Boags, Thomas J. Piggot, Syma Khalid

Volume 95, Issue 9, Pages (November 2008)

Volume 89, Issue 1, Pages 3-13 (July 2005)

Sundeep S. Deol, Peter J. Bond, Carmen Domene, Mark S.P. Sansom

Structure and Topology of the Huntingtin 1–17 Membrane Anchor by a Combined Solution and Solid-State NMR Approach Matthias Michalek, Evgeniy S. Salnikov,

Desmosterol May Replace Cholesterol in Lipid Membranes

Site-Directed Spin-Labeling Study of the Light-Harvesting Complex CP29

Volume 95, Issue 9, Pages (November 2008)

Grischa R. Meyer, Justin Gullingsrud, Klaus Schulten, Boris Martinac

Cholesterol Modulates the Dimer Interface of the β2-Adrenergic Receptor via Cholesterol Occupancy Sites Xavier Prasanna, Amitabha Chattopadhyay, Durba.

Volume 96, Issue 3, Pages (February 2009)

Shelly Tzlil, Diana Murray, Avinoam Ben-Shaul Biophysical Journal

Insight into Early-Stage Unfolding of GPI-Anchored Human Prion Protein

Volume 83, Issue 3, Pages (September 2002)

Volume 83, Issue 6, Pages (December 2002)

Volume 114, Issue 3, Pages (February 2018)

Ion-Induced Defect Permeation of Lipid Membranes

Robust Driving Forces for Transmembrane Helix Packing

Scott M. Blackman, Eric J. Hustedt, Charles E. Cobb, Albert H. Beth

Vesna Serrano, Wenge Liu, Stefan Franzen Biophysical Journal

Chze Ling Wee, David Gavaghan, Mark S.P. Sansom Biophysical Journal

Jochen Zimmer, Declan A. Doyle, J. Günter Grossmann

Membrane Perturbation Induced by Interfacially Adsorbed Peptides

Yinon Shafrir, Stewart R. Durell, H. Robert Guy Biophysical Journal

Volume 98, Issue 4, Pages (February 2010)

Presentation transcript:

Defects in Vesicle Core Induced by Escherichia coli Dihydroorotate Dehydrogenase Sheila G. Couto, M. Cristina Nonato, Antonio J. Costa-Filho Biophysical Journal Volume 94, Issue 5, Pages 1746-1753 (March 2008) DOI: 10.1529/biophysj.107.120055 Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 1 ESR spectra of spin labels (a) DPPTC, (b) 5-, (c) 10-, (d) 12-, and (e) 16-PC incorporated into vesicles of DOPC/Triton X-100 in the absence (solid line) and in the presence of EcDHODH (dashed line). Experimental conditions: microwave frequency 9.5GHz; modulation amplitude 1.0G; modulation frequency 100kHz; microwave power 10 mW. Biophysical Journal 2008 94, 1746-1753DOI: (10.1529/biophysj.107.120055) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 2 Chemical structures of the (a) acyl-chain (16-PC) and (b) headgroup (DPPTC) spin labels showing the principal magnetic axes (xm, ym, zm). Biophysical Journal 2008 94, 1746-1753DOI: (10.1529/biophysj.107.120055) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 3 Experimental (dashed line) and simulated (solid line) ESR spectra from (a) DPPTC, (b) 12-, and (c) 16-PC labels in mixtures of DOPC/Triton X-100 (left column) and DOPC/Triton X-100/EcDHODH (right column). Biophysical Journal 2008 94, 1746-1753DOI: (10.1529/biophysj.107.120055) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 4 Experimental (dashed line) and calculated (solid line) ESR spectra from 5-PC spin probe in mixtures of (a) DOPC/Triton X-100 and (b) DOPC/Triton X-100/EcDHODH. b shows the individual components: 1 (bulk lipid, dotted line) and 2 (boundary lipid, dash-dotted line) obtained by means of NLSL simulations. Biophysical Journal 2008 94, 1746-1753DOI: (10.1529/biophysj.107.120055) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 5 Experimental (dashed line) and calculated (solid line) ESR spectra from 10-PC spin probe in mixtures of (a) DOPC/Triton X-100 and (b) DOPC/Triton X-100/EcDHODH. b shows the individual components: 1 (bulk lipid, dotted line) and 2 (boundary lipid, dash-dotted line) obtained as described in the text. Biophysical Journal 2008 94, 1746-1753DOI: (10.1529/biophysj.107.120055) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 6 Ribbon representation of EcDHODH structure emphasizing the hydrophobicity pattern for N-terminal domain (comprising residues in the two α-helices and one 310 helix) as determined by ProtScale software. The dehydrogenase-active domain is shown in cyan. Hydrophobic and hydrophilic residues in the N-terminal domain are colored in dark and light gray, respectively. Biophysical Journal 2008 94, 1746-1753DOI: (10.1529/biophysj.107.120055) Copyright © 2008 The Biophysical Society Terms and Conditions