Is sperm cryopreservation an option for fertility preservation in patients with spinal cord injury-induced anejaculation?  Barbara Ferreira da Silva, B.Sc., Milton Borrelli, M.D., Roberta Maria Fariello, B.Sc., Adriana Ester Restelli, Ph.D., Paula Toni Del Giudice, B.Sc., Deborah Montagnini Spaine, Ph.D., Ricardo Pimenta Bertolla, D.V.M., Ph.D., Agnaldo Pereira Cedenho, M.D., Ph.D.  Fertility and Sterility  Volume 94, Issue 2, Pages 564-573 (July 2010) DOI: 10.1016/j.fertnstert.2009.03.022 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Photomicrography of sperm submitted to the alkaline comet assay, stained with ethidium bromide and visualized under fluorescent microscopy using a 400× magnification. Comet class I represents sperm with intact or nonmigrating DNA, Comet class II represents sperm with little or no DNA fragmentation, Comet class III represents sperm with more intense DNA fragmentation, and Comet class IV represents sperm with high DNA fragmentation. Fertility and Sterility 2010 94, 564-573DOI: (10.1016/j.fertnstert.2009.03.022) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Photomicrography of sperm submitted to the DAB mitochondrial activity assay visualized under brightfield microscopy using a 1,000× magnification in oil. DAB class I represents sperm with only active mitochondria, DAB class II represents sperm with mostly active mitochondria, DAB class III represents sperm with mostly inactive mitochondria, and DAB class IV represents sperm with only inactive mitochondria. Fertility and Sterility 2010 94, 564-573DOI: (10.1016/j.fertnstert.2009.03.022) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions