Mass Spectrometry.

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Mass Spectrometry.

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Presentation transcript:

Mass Spectrometry

Analytical Chemistry • Chromatography (Separations) • Mass Spectrometry • Infrared (IR) Spectroscopy • Nuclear Magnetic Resonance (NMR) Spectroscopy • X-ray Crystallography (visual solid state molecular structure)

Mass Spectrometry What insights are we hoping to gain? • Molecular Mass/Isotopic Distribution • Molecular Formula • Some Structural Characteristics – Fragmentation Pattern

Mass Spectromeric Isotope Indentification Only molecules of the correct m/z (mass/charge) will make it to the detector.

EI-MS of toluene

EI-MS of 2,2-Dimethyl Pentane

EI-MS of 2,2-Dimethyl Pentane Peaks with smaller m/z values __ called fragment ion peaks __ represent positively charged fragments of the parent molecule

EI-MS of 2,2-Dimethyl Pentane

EI-MS of n-Propanol

EI-MS of 4-Heptanone

EI-MS of 4-Heptanone

EI-MS of 4-Heptanone

Isotopes

EI-MS of Benzyl Bromide

Isotopes

EI-MS of Benzyl Bromide

EI-MS of 4-Chloroanisole

Isotopes

Isotopes