Decellularization reduces immunogenicity of sheep pulmonary artery vascular patches Eric J. Lehr, MD, PhD, Gina R. Rayat, PhD, Brian Chiu, MD, Thomas.

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Presentation transcript:

Decellularization reduces immunogenicity of sheep pulmonary artery vascular patches Eric J. Lehr, MD, PhD, Gina R. Rayat, PhD, Brian Chiu, MD, Thomas Churchill, PhD, Locksley E. McGann, PhD, James Y. Coe, MD, David B. Ross, MD The Journal of Thoracic and Cardiovascular Surgery Volume 141, Issue 4, Pages 1056-1062 (April 2011) DOI: 10.1016/j.jtcvs.2010.02.060 Copyright © 2011 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 Gross pathology of decellularized patch implanted for 6 months at the time of explantation in situ (A) and immediately after explantation (B). The Journal of Thoracic and Cardiovascular Surgery 2011 141, 1056-1062DOI: (10.1016/j.jtcvs.2010.02.060) Copyright © 2011 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 Photomicrographs of representative sections of nondecellularized and decellularized allogeneic ovine pulmonary artery patches at the time of implantation (A and B) and 4 weeks after implantation (C–J). Samples were fixed in formaldehyde, embedded in paraffin, and sectioned at 5 μm. A and B represent grafts at the time of implantation (original magnification 40×). Solid arrows and open arrows indicate the external and internal lamina, respectively. Early and intense lymphocytic infiltrates are profuse in nondecellularized patches but virtually absent in decellularized grafts (C and D, original magnification 40×). Both decellularized and nondecellularized grafts show thickening of the intima and adventitia, as well as disruption of the external elastic lamina with myofibroblastic proliferation (Movat's pentachrome stain, E and F, original magnification 40×). Neovascularization of the adventitia and media with thickened vessels was also detected (von Willebrand factor positive, G and H; CD31+, I and J; original magnification 100×). B cells (CD79+, K and L, original magnification 100×) were identified in the perivascular regions at the medial–adventitial junction in nondecellularized control grafts at 6 months. CRYO, Cryopreserved; CRYO & DECEL, cryopreserved and decellularized. The Journal of Thoracic and Cardiovascular Surgery 2011 141, 1056-1062DOI: (10.1016/j.jtcvs.2010.02.060) Copyright © 2011 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 Scanning electron microscopy of the endothelial surface of explanted nondecellularized and decellularized pulmonary artery patches at 4 weeks after implantation. Both nondecellularized control (A and B) and decellularized (C) patches show re-endothelialization, but white blood cell infiltrate is identified only on nondecellularized control grafts. The bar indicates 10 μm in all electron micrographs. The Journal of Thoracic and Cardiovascular Surgery 2011 141, 1056-1062DOI: (10.1016/j.jtcvs.2010.02.060) Copyright © 2011 The American Association for Thoracic Surgery Terms and Conditions

Figure 4 Flow cytometric determination of the humoral response of sheep receiving allograft pulmonary artery patches. Recipient generation of anti-donor IgG peaks at 2 months after implantation. Decellularization of donor pulmonary artery patches eliminates the development of the recipient humoral immune response. 2° Ab, Secondary antibody alone; FITC, fluorescein isothiocyanate. The Journal of Thoracic and Cardiovascular Surgery 2011 141, 1056-1062DOI: (10.1016/j.jtcvs.2010.02.060) Copyright © 2011 The American Association for Thoracic Surgery Terms and Conditions