In search of an efficient injection technique for future clinical application of spermatogonial stem cell transplantation: infusion of contrast dyes in.

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In search of an efficient injection technique for future clinical application of spermatogonial stem cell transplantation: infusion of contrast dyes in isolated cadaveric human testes Liang Ning, M.D., Ph.D., Jie Meng, M.D., Ellen Goossens, Ph.D., Tony Lahoutte, M.D., Ph.D., Miriam Marichal, M.D., Ph.D., Herman Tournaye, M.D., Ph.D. Fertility and Sterility Volume 98, Issue 6, Pages 1443-1448.e1 (December 2012) DOI: 10.1016/j.fertnstert.2012.08.023 Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 1 (A) Injection of contrast liquid guided by ultrasonography. A 23-gauge needle (A1–A3, arrow) was inserted into the mediastinum (rete testis), the liquid containing microbubble was infused into the testis (A4 arrow; diameter of the tubule, 180 μm). (B) Micro-computerized tomography shows the staining of contrast after injection through different sites: through the deferent duct (B1); through the head of the epididymis (B2); and different dosages through the rete testis (B3: 1 × 800 μL; B4: 2 × 400 μL). Fertility and Sterility 2012 98, 1443-1448.e1DOI: (10.1016/j.fertnstert.2012.08.023) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Testis of patient 3 injected with Chinese ink through the rete testis. (A) Overview of a testis section. Ink particles were observed in the lumen of the rete testis (B) and the seminiferous tubules, close to the rete testis (C). No ink particles were observed in the lumen of the epididymis (D). In the testes of patient 2, ink particles could be observed in the lumen of the epididymis (E) and the interstitium close (F) and distant (G) from the rete testis, indicating liquid reflux and leakage during infusions. Fertility and Sterility 2012 98, 1443-1448.e1DOI: (10.1016/j.fertnstert.2012.08.023) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 Set-up for infusion. Transfusion was performed under gravity by positioning a syringe at 75 cm above the testis. A 23-gauge needle was inserted close to the caput epididymis into the rete testis under continuous monitoring by ultrasonography. A total dose of 800 μL mixed contrast was transfused in 2.5–4.5 minutes. Fertility and Sterility 2012 98, 1443-1448.e1DOI: (10.1016/j.fertnstert.2012.08.023) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions