Silke Lassmann, Ulrike V

Slides:

Advertisements

Similar presentations

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.

Advertisements

CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.

Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis Philippe Szankasi, David.

Immunoglobulin Gene Mutations and Frequent Use of VH1-69 and VH4-34 Segments in Hepatitis C Virus-Positive and Hepatitis C Virus-Negative Nodal Marginal.

Quantitative Detection and Differentiation of Human Herpesvirus 6 Subtypes in Bone Marrow Transplant Patients by Using a Single Real-Time Polymerase Chain.

Somatic Mutation of the 5′ Noncoding Region of the BCL-6 Gene Is Associated with Intraclonal Diversity and Clonal Selection in Histological Transformation.

Detection of Exon 12 Mutations in the JAK2 Gene

Direct DNA Amplification from Crude Clinical Samples Using a PCR Enhancer Cocktail and Novel Mutants of Taq Zhian Zhang, Milko B. Kermekchiev, Wayne.

Effectiveness of Capillary Electrophoresis Using Fluorescent-Labeled Primers in Detecting T-Cell Receptor γ Gene Rearrangements Timothy C. Greiner, Ronald.

RT-PCR Analysis of RNA Extracted from Bouin-Fixed and Paraffin-Embedded Lymphoid Tissues Annunziata Gloghini, Barbara Canal, Ulf Klein, Luigino Dal Maso,

Direct DNA Amplification from Crude Clinical Samples Using a PCR Enhancer Cocktail and Novel Mutants of Taq Zhian Zhang, Milko B. Kermekchiev, Wayne.

The Frequency of Immunoglobulin Heavy Chain Gene and T-Cell Receptor γ-Chain Gene Rearrangements and Epstein-Barr Virus in ALK+ and ALK− Anaplastic Large.

Natalia Kholod, Jacques Boniver, Philippe Delvenne

Whole Genome Amplification for Array Comparative Genomic Hybridization Using DNA Extracted from Formalin-Fixed, Paraffin-Embedded Histological Sections

Detection of Clonally Restricted Immunoglobulin Heavy Chain Gene Rearrangements in Normal and Lesional Skin Minakshi Nihal, Debra Mikkola, Gary S. Wood

Successful Application of Microarray Technology to Microdissected Formalin-Fixed, Paraffin-Embedded Tissue Renata A. Coudry, Sibele I. Meireles, Radka.

Analysis of T-Cell Clonality Using Laser Capture Microdissection and High-Resolution Microcapillary Electrophoresis Evgeny Yakirevich, Cynthia L. Jackson,

Comparison of BIOMED-2 Versus Laboratory-Developed Polymerase Chain Reaction Assays for Detecting T-Cell Receptor-γ Gene Rearrangements Keyur P. Patel,

Immunoguided Laser Assisted Microdissection Techniques for DNA Methylation Analysis of Archival Tissue Specimens Franziska C. Eberle, Jeffrey C. Hanson,

Jonathan A. Schumacher, Stephen D. Jenson, Kojo S. J

Isothermal Multiple Displacement Amplification

IgH PCR of Zinc Formalin-Fixed, Paraffin-Embedded Non-Lymphomatous Gastric Samples Produces Artifactual “Clonal” Bands Not Observed in Paired Tissues.

Betaine, Dimethyl Sulfoxide, and 7-Deaza-dGTP, a Powerful Mixture for Amplification of GC-Rich DNA Sequences Marco Musso, Renata Bocciardi, Sara Parodi,

Yanggu Shi, Sharon F. Terry, Patrick F. Terry, Lionel G

B-Cell Clonality Determination Using an Immunoglobulin κ Light Chain Polymerase Chain Reaction Method Reetesh K. Pai, Artemis E. Chakerian, John M. Binder,

Association of Clinical Status of Follicular Lymphoma Patients after Autologous Stem Cell Transplant and Quantitative Assessment of Lymphoma in Blood.

Performance Evaluation of Two Methods Using Commercially Available Reagents for PCR-Based Detection of FMR1 Mutation Jane S. Juusola, Paula Anderson,

Quantitative mRNA Expression Analysis from Formalin-Fixed, Paraffin-Embedded Tissues Using 5′ Nuclease Quantitative Reverse Transcription-Polymerase Chain.

Detection of Central Nervous System Leukemia in Children with Acute Lymphoblastic Leukemia by Real-Time Polymerase Chain Reaction Sharon R. Pine, Changhong.

Protocol for the Use of Polymerase Chain Reaction in the Detection of Intraocular Large B-Cell Lymphoma in Ocular Samples Aires Lobo, Narciss Okhravi,

Molecular Diagnostic Approach to Non-Hodgkin's Lymphoma

Long-Range (17.7 kb) Allele-Specific Polymerase Chain Reaction Method for Direct Haplotyping of R117H and IVS-8 Mutations of the Cystic Fibrosis Transmembrane.

Molecular Typing of West Nile Virus, Dengue, and St

Rapid and Accurate Detection of Monoclonal Immunoglobulin Heavy Chain Gene Rearrangement by DNA Melting Curve Analysis in the LightCycler System Dongsheng.

PCR Analysis of the Immunoglobulin Heavy Chain Gene in Polyclonal Processes Can Yield Pseudoclonal Bands as an Artifact of Low B Cell Number Kojo S.J.

Christine L. Baker, Cecily P. Vaughn, Wade S. Samowitz

Sean C. Murphy, Daniel R. Hoogestraat, Dhruba J

Rapid Detection of Clonal T-Cell Receptor-β Gene Rearrangements in T-Cell Lymphomas Using the LightCycler-Polymerase Chain Reaction with DNA Melting Curve.

Brenton T. Tan, Roger A. Warnke, Daniel A. Arber

SeqSharp The Journal of Molecular Diagnostics

Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis Philippe Szankasi, David.

Detection of Exon 12 Mutations in the JAK2 Gene

Development and Clinical Implementation of a Combination Deletion PCR and Multiplex Ligation-Dependent Probe Amplification Assay for Detecting Deletions.

Catherine E. Keegan, Anthony A. Killeen

Heteroduplex Formation in SMN Gene Dosage Analysis

Analytical Detection of Immunoglobulin Heavy Chain Gene Rearrangements in Gastric Lymphoid Infiltrates by Peak Area Analysis of the Melting Curve in the.

A Rare Mutation in the Primer Binding Region of the Amelogenin Gene Can Interfere with Gender Identification Bonnie Shadrach, Mairead Commane, Carol.

Detection of Genomic Variations in BRCA1 and BRCA2 Genes by Long-Range PCR and Next-Generation Sequencing Imma Hernan, Emma Borràs, Miguel de Sousa Dias,

Larissa V. Furtado, Helmut C. Weigelin, Kojo S. J

Allison M. Cushman-Vokoun, Solomon Connealy, Timothy C. Greiner

N. Scott Reading, Stephen D. Jenson, Jeffrey K. Smith, Megan S

A Novel Long-Range PCR Sequencing Method for Genetic Analysis of the Entire PKD1 Gene Ying-Cai Tan, Alber Michaeel, Jon Blumenfeld, Stephanie Donahue,

Ye Bang-Ce, Chu Xiaohe, Fan Ye, Li Songyang, Yin Bincheng, Zuo Peng

Jianbo Song, Danielle Mercer, Xiaofeng Hu, Henry Liu, Marilyn M. Li

David L. Evers, Junkun He, Yeon Ho Kim, Jeffrey T. Mason, Timothy J

The Detection of t(14;18) in Archival Lymph Nodes

T Cell Receptor γ-Chain Gene Polymerase Chain Reaction to Diagnose Central Nervous System Involvement by Cutaneous T Cell Lymphoma Robert Taylor, Jo-Anne.

Multiplex PCR Detection of GSTM1, GSTT1, and GSTP1 Gene Variants

Feras M. Hantash, Arlene Rebuyon, Mei Peng, Joy B

The Distribution of Gene Segments in T-Cell Receptor γ Gene Rearrangements Demonstrates the Need for Multiple Primer Sets Lyle C. Lawnicki, Ronald J.

Rapid and Accurate Detection of Monoclonal Immunoglobulin Heavy Chain Gene Rearrangement by DNA Melting Curve Analysis in the LightCycler System Dongsheng.

Improved Detection of KIT Exon 11 Duplications in Formalin-Fixed, Paraffin-Embedded Gastrointestinal Stromal Tumors Jerzy Lasota, Bartosz Wasag, Sonja.

Sensitive Detection of Polyalanine Expansions in PHOX2B by Polymerase Chain Reaction Using Bisulfite-Converted DNA Hidekazu Horiuchi, Ayako Sasaki, Motoki.

Analytical Evaluation of Primer Engineered Multiplex Polymerase Chain Reaction– Restriction Fragment Length Polymorphism for Detection of Factor V Leiden.

Pseudo-Spikes Are Common in Histologically Benign Lymphoid Tissues

Detection of BRCA1 and BRCA2 Ashkenazi Jewish Founder Mutations in Formalin- Fixed Paraffin-Embedded Tissues Using Conventional PCR and Heteroduplex/Amplicon.

Effects of Electron-Beam Irradiation on Buccal-Cell DNA

BIOMED-2 Multiplex Immunoglobulin/T-Cell Receptor Polymerase Chain Reaction Protocols Can Reliably Replace Southern Blot Analysis in Routine Clonality.

Maureen J. O'Sullivan, Peter A. Humphrey, Louis P. Dehner, John D

Quantification of bcl-2/JH Fusion Sequences and a Control Gene by Multiplex Real- Time PCR Coupled with Automated Amplicon Sizing by Capillary Electrophoresis

Presentation transcript:

Application of BIOMED-2 Primers in Fixed and Decalcified Bone Marrow Biopsies Silke Lassmann, Ulrike V. Gerlach, Katja Technau-Ihling, Martin Werner, Paul Fisch The Journal of Molecular Diagnostics Volume 7, Issue 5, Pages 582-591 (November 2005) DOI: 10.1016/S1525-1578(10)60591-0 Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Performance of different DNA extracts in PCR analysis of two fragments of the β-Globin gene. a: PCR amplification and agarose gel electrophoresis for a 268-bp fragment and 410-bp fragment of the β-Globin gene using 1) crude DNA lysates (left panel), 2) crude DNA lysates further column-purified (middle panel), and 3) DNeasy-purified DNA extracts (right panel). b: PCR amplification of the 268-bp fragment and a 536-bp fragment of the β-Globin gene using DNeasy-purified DNA extracts from formalin- and glutardialdehyde-fixed, decalcified bone marrow biopsies. The Journal of Molecular Diagnostics 2005 7, 582-591DOI: (10.1016/S1525-1578(10)60591-0) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Representative results of the modified BIOMED-2 PCR reactions for IgH clonality analysis of the FRII and FRIII region. Top panels: Genescan analysis of PCR products obtained from FRII (left) and FRIII (right) analysis, with fixed and decalcified bone marrow samples with histologically proven B-cell lymphoma (a–e), bone marrow sample with a reactive lymphoid infiltrate (f), formalin-fixed lymph node sample with histologically proven B-cell lymphoma (g), and negative control (h; water). Arrows indicate clonal peaks within the expected size range. Bottom panels: Heteroduplex analysis of the same PCR products (a–h) as shown in the top panels. Left gel: FRII PCR products; right gel: FRIII PCR products. The Journal of Molecular Diagnostics 2005 7, 582-591DOI: (10.1016/S1525-1578(10)60591-0) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions