Development and prevention of postsurgical adhesions in a chimeric mouse model of experimental endometriosis  Jennifer L. Herington, Ph.D., Marta A. Crispens, M.D., Alessandra C. Carvalho-Macedo, M.D., Aroldo Fernando Camargos, M.D., Ph.D., Dan I. Lebovic, M.A., M.D., Kaylon L. Bruner-Tran, Ph.D., Kevin G. Osteen, Ph.D.  Fertility and Sterility  Volume 95, Issue 4, Pages 1295-1301.e1 (March 2011) DOI: 10.1016/j.fertnstert.2010.09.017 Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Gross morphology of experimental lesions and adhesions and timing of tissue injection. (A and B) Gross photomicrographs of lesions (solid arrows) and adhesions (dashed arrows) identified in mice at the site of ovariectomy. Human endometrial tissues were injected (A) 4 hours or (B) 5 days after surgery. Mice were killed 5 days after tissue injection. Adhesion formation decreased as time from surgery increased. (C) Left uterine horn (LUH) of an intact disease-free mouse. (D and E) Gross photomicrographs of adhesions identified at sites distal from the surgical injury in mice receiving human tissues 16 hours after surgery. (D) Omentum adhered to the colon. (E) Large adhesion within the mesentery. Original magnification ×15. Fertility and Sterility 2011 95, 1295-1301.e1DOI: (10.1016/j.fertnstert.2010.09.017) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Gross morphology and assessment of microvessel density in experimental endometriosis. (A and B) Gross photomicrographs of lesions (solid arrows) and adhesions (dashed arrows) at the site of the surgical injury in (A) control mice and (B) PIO-treated mice that also received tissues treated with E2 + PIO. Human endometrial tissues were injected 16 hours after ovariectomy. Assessment of microvessel density (MVD) by immunohistochemical localization of von Willebrand factor in (C and D) experimental endometriosis lesions and (E and F) adhesions from the same mice. (C and E) Control mice received E2-treated tissue, and (D and F) PIO-treated mouse received tissue treated with E2 + PIO. Original magnification ×100. Fertility and Sterility 2011 95, 1295-1301.e1DOI: (10.1016/j.fertnstert.2010.09.017) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Timing of postsurgical tissue injection and collection Timing of postsurgical tissue injection and collection. Schematic diagram representing the timing of surgery before tissue injection. Surgeries were adjusted to allow different mice to receive shared human endometrial tissue injected the same fixed time point. Killing of all mice was performed at 5 days (horizontal bars) after tissue injection, and peritoneal ectopic lesions and adhesions were then assessed. Fertility and Sterility 2011 95, 1295-1301.e1DOI: (10.1016/j.fertnstert.2010.09.017) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions