PPARγ signaling correlates with LATS2 in human and mouse tumors and promotes cell death in a LATS2-dependent manner. PPARγ signaling correlates with LATS2.

Slides:



Advertisements
Similar presentations
B Supplementary Fig S1. (A) ZR75- and MCF7-PELP1 knockdown cells were generated as described in methods section. Pooled colonies were analyzed for PELP1.
Advertisements

Cell Physiol Biochem 2013;32: DOI: /
Volume 9, Issue 2, Pages (August 2017)
Rab11-FIP3 silencing impairs T cell activation events.
Tumor-Derived Jagged1 Promotes Osteolytic Bone Metastasis of Breast Cancer by Engaging Notch Signaling in Bone Cells  Nilay Sethi, Xudong Dai, Christopher.
MEF Promotes Stemness in the Pathogenesis of Gliomas
Volume 30, Issue 2, Pages (August 2016)
Supplementary Table 1 Sequences of the primer used for q-PCR analysis
Volume 28, Issue 5, Pages (November 2015)
Volume 2, Issue 4, Pages (April 2008)
AKR1C3 is a biomarker of sensitivity to PR-104 in preclinical models of T-cell acute lymphoblastic leukemia by Donya Moradi Manesh, Jad El-Hoss, Kathryn.
Modulation of K-Ras-Dependent Lung Tumorigenesis by MicroRNA-21
(A) ZR75-1 cells were treated with 1 μM 5-aza-2′-deoxycytidine (5-Aza) for 4 d. (A) ZR75-1 cells were treated with 1 μM 5-aza-2′-deoxycytidine (5-Aza)
Molecular Therapy - Nucleic Acids
Combined PLX3397 and PTX treatment inhibits metastasis in a CD8-dependent manner. Combined PLX3397 and PTX treatment inhibits metastasis in a CD8-dependent.
Volume 30, Issue 2, Pages (August 2016)
Volume 6, Issue 5, Pages (March 2014)
Volume 14, Issue 3, Pages (March 2014)
Volume 29, Issue 4, Pages (April 2016)
LATS2 is a tumor suppressor in a mouse lumB breast cancer model.
(A) Schematic representation of the conditional Lats1 locus.
Volume 28, Issue 4, Pages (October 2015)
B7-H4 expression correlates with MHC-I expression and improved prognosis in patients with breast cancer. B7-H4 expression correlates with MHC-I expression.
Volume 23, Issue 3, Pages (February 2013)
Deletion of Lats1 is phenotypically distinct from Lats2 deletion in PyMT tumors. Deletion of Lats1 is phenotypically distinct from Lats2 deletion in PyMT.
Molecular Therapy - Nucleic Acids
Inhibition of KLF4 by Statins Reverses Adriamycin-Induced Metastasis and Cancer Stemness in Osteosarcoma Cells  Yangling Li, Miao Xian, Bo Yang, Meidan.
LATS2 promotes death of lumB cells.
Volume 9, Issue 2, Pages (August 2017)
Volume 47, Issue 5, Pages e4 (November 2017)
Minchul Kim, Taekhoon Kim, Randy L. Johnson, Dae-Sik Lim  Cell Reports 
Nucleolar poly-GR/PR alter nucleolar organization and inhibit translation. Nucleolar poly-GR/PR alter nucleolar organization and inhibit translation. GFP,
Jungmook Lyu, Vicky Yamamoto, Wange Lu  Developmental Cell 
Molecular Therapy - Methods & Clinical Development
FOXO3a Is Activated in Response to Hypoxic Stress and Inhibits HIF1-Induced Apoptosis via Regulation of CITED2  Walbert J. Bakker, Isaac S. Harris, Tak.
(A) Genotyping of the Lats2 and Cre alleles in the PyMT-derived cell lines (see primers location in Fig S2A). (A) Genotyping of the Lats2 and Cre alleles.
Knockdown of DAO inhibits DNA damage–induced senescence.
Volume 16, Issue 2, Pages (August 2012)
Volume 23, Issue 1, Pages (April 2018)
Volume 7, Issue 1, Pages (January 2008)
Down-regulation of LATS1 promotes the formation of tumors enriched in basal-like features. Down-regulation of LATS1 promotes the formation of tumors enriched.
Anti-CD20 CAR exPBNK significantly inhibit growth of Raji cells in xenografted mice. Anti-CD20 CAR exPBNK significantly inhibit growth of Raji cells in.
Volume 57, Issue 6, Pages (March 2015)
Volume 17, Issue 12, Pages (December 2016)
Volume 12, Issue 4, Pages (July 2015)
Volume 167, Issue 2, Pages e9 (October 2016)
TRAF4 is required for EGFR activation in response to EGF stimulation.
ADAM8 is induced by hypoxia and promotes angiogenesis ASixteen h after plating, cells were cultured under normoxic (−) or hypoxic (+, 1% O2) conditions.
LATS2-associated gene expression pattern is down-regulated specifically in lumB breast tumors. LATS2-associated gene expression pattern is down-regulated.
Metascape Tripathi et al 2015pathways enrichment analysis (Tripathi et al 2015, of differentially expressed genes (counts > 5, FC.
(A) Schematic representation of the conditional Lats2 locus.
(A) Distribution of LATS2 mRNA expression levels in different breast cancer subtypes (PAM50, METABTIC dataset); ***P-value < 0.001, t test comparing lumB.
Chd5 deficiency leads to compromised expression of the repressive histone mark H3K27me3 and up-regulation of ribosomal protein genes. Chd5 deficiency leads.
Ventral mouse V-SVZ cells exhibit higher mTORC1 activity than dorsal cells and are responsive to rapamycin. Ventral mouse V-SVZ cells exhibit higher mTORC1.
ArfGAP1 expression alters GBF1 recruitment to Golgi membranes.
Validation of GFP and BMP-2 expression in lentivirus-transduced neonatal human skin fibroblasts. Validation of GFP and BMP-2 expression in lentivirus-transduced.
(A) Relative expression levels of the top most down-regulated genes in LATS2L breast tumors (TCGA-BRCA dataset, see Fig 1) in the panel of breast cancer.
Chd5-deficient NSCs generate excessive astrocytes at the expense of neurons. Chd5-deficient NSCs generate excessive astrocytes at the expense of neurons.
The BRCA1 aggregates exclude large nuclear structures.
Ectopic expression of caveolin-1 in ZR75 human breast cancer cells downregulated survivin and decreased cell proliferation. Ectopic expression of caveolin-1.
Association of NM-HA and NM-GFP with SGs is transient.
LATS2 augments oxidative phosphorylation.
Volume 26, Issue 12, Pages e4 (March 2019)
Expression of Chx10-cre and phenotypes of Chx10-cre induced Smo ablation. Expression of Chx10-cre and phenotypes of Chx10-cre induced Smo ablation. A–G,
EN1 expression in breast cancer and clinical outcome.
ARID1A promotes DSB end resection.
RUNX3 depletion induces cellular senescence and inflammatory cytokine expression in cells undergoing TGFβ-mediated EMT. A, Cells were transfected with.
The interaction of PALB2 with BRCA1 is required for the assembly of PALB2, BRCA2, and RAD51 nuclear foci. The interaction of PALB2 with BRCA1 is required.
Volume 26, Issue 11, Pages e6 (March 2019)
EC-derived SP cells are targeted by CD30.CAR T cells.
Presentation transcript:

PPARγ signaling correlates with LATS2 in human and mouse tumors and promotes cell death in a LATS2-dependent manner. PPARγ signaling correlates with LATS2 in human and mouse tumors and promotes cell death in a LATS2-dependent manner. (A) KEGG pathways significantly enriched within the list of genes commonly down-regulated in Lats2-CKO PyMT (compared with WT-PyMT) tumors and LATS2L lumB human tumors (TCGA). (B) RT-qPCR quantification of Pparg and Plin1 expression in tumors derived from 3 mo old Lats2-CKO PyMT (n = 9) and WT-PyMT littermate controls (n = 6); mean ± SEM; *P-value < 0.05, ***P-value < 0.001. (C) Expression levels of the indicated genes (left) and proteins (right) in cultured WT PyMT and Lats2-CKO PyMT tumor-derived cells stably transduced with LATS2 or vector control; mean ± SD of 2 technical repeats. (D) ZR75-1 cells were transfected with GFP-LATS2 or control GFP plasmid, stained 48 h later with anti-PPARγ and cleaved caspase 3 (CC3) antibodies, and subjected to imaging flow cytometry (ImageStreamX). Only GFP-positive cells with intact nuclei were analyzed. Cells with nuclear localization of the transfected protein were identified by similarity between GFP and DAPI staining. Percentage of cells positively stained for PPARγ in each subpopulation is presented. Representative images are shown at the bottom. BF = bright field. (E) Left panel: scatter plot depicting PPARG and LATS2 expression in luminal tumors in the TCGA-BRCA dataset (n = 613). Right panel: GSEA of genes ranked according to expression fold change between LATS2L tumors and LATSH tumors in the TCGA lumB dataset. (F) Kaplan-Meier analysis of survival probability of luminal breast cancer patients (METABRIC dataset, n = 1139; Cox proportional hazards model) defined according to expression levels of LATS2 and PPARG.(G) RT-qPCR quantification of PPARG and LPL (left) and LATS2 (right) mRNA in ZR75-1 cells treated for 48 h with increasing concentrations of RGZ (0, 10, 25, 50, and 100 μM, respectively); mean ± SD of two technical replicates. (H) ZR75-1/vector (vector) and ZR75-1/LATS2 (LATS2) cells were treated with 100 μM RGZ for 48 h, followed by PI exclusion analysis; mean ± SEM of the ratio between % PI+ cells in each cell type, from three independent experiments. (I) Cell lines derived from WT-PyMT and Lats2-CKO PyMT tumors were treated with 100 μM RGZ for 48 h, followed by PI exclusion analysis; mean ± SEM of the ratio between % PI+ cells in treated versus untreated cells, from two independent experiments. Noa Furth et al. LSA 2018;1:e201800171 © 2018 Furth et al.