The disturbance of TH17-Treg cell balance in adenomyosis

Slides:

Advertisements

Similar presentations

TAGLN expression is deregulated in endometriosis and may be involved in cell invasion, migration, and differentiation Gabriela dos Santos Hidalgo, M.Sc.,

Advertisements

Sphingosine pathway deregulation in endometriotic tissues

Immune-inflammation gene signatures in endometriosis patients

Association of abdominal obesity, insulin resistance, and oxidative stress in adipose tissue in women with polycystic ovary syndrome Li Chen, M.S., Wen.

Zhao Jing, M. D. , Zhang Qiong, M. D. , Wang Yonggang, M. D

Increased expression of c-fos protein associated with increased matrix metalloproteinase-9 protein expression in the endometrium of endometriotic patients

Constitutive and tumor necrosis factor-α-induced activation of nuclear factor-κB in adenomyosis and its inhibition by andrographolide Bin Li, M.S., Ming.

Increased expression of macrophage colony–stimulating factor and its receptor in patients with endometriosis Nicole M. Budrys, M.D., M.P.H., Hareesh.

Different types of small nerve fibers in eutopic endometrium and myometrium in women with endometriosis Natsuko Tokushige, Ph.D., Robert Markham, Ph.D.,

Interleukin-17 and Prostaglandin E2 Are Involved in Formation of an M2 Macrophage- Dominant Microenvironment in Lung Cancer Lunxu Liu, MD, PhD, Dongxia.

Dendritic cells in semen of infertile men: association with sperm quality and inflammatory status of the epididymis Yong-Gang Duan, M.D., Qiongli Zhang,

Eutopic endometrial interleukin-18 system mRNA and protein expression at the level of endometrial-myometrial interface in adenomyosis patients Hong-Yuan.

Maria L. Barcena de Arellano, M. S. , Jeannette Oldeweme, M. D

Relationship between apoptosis and the number of macrophages in eutopic endometrium from women with and without endometriosis Donald P Braun, Ph.D.,

Serum galectin-9 as a noninvasive biomarker for the detection of endometriosis and pelvic pain or infertility-related gynecologic disorders Reka Brubel,

Human leukocyte antigen-G, a ligand for the natural killer receptor KIR2DL4, is expressed by eutopic endometrium only in the menstrual phase Masaaki.

Patrizia Carrarelli, Ph. D. , Chih-Fen Yen, M. D. , Felice Arcuri, Ph

Eutopic and ectopic stromal cells from patients with endometriosis exhibit differential invasive, adhesive, and proliferative behavior Ali-Akbar Delbandi,

Gentaro Izumi, Ph. D. , M. D. , Kaori Koga, Ph. D. , M. D

Volume 20, Issue 12, Pages (September 2017)

Expression of motility-related molecule Cdc42 in endometrial tissue in women with adenomyosis and ovarian endometriomata Gaia Goteri, M.D., Andrea Ciavattini,

Regulatory T cells, inflammation, and endoplasmic reticulum stress in women with defective endometrial receptivity Mario Galgani, Ph.D., Luigi Insabato,

Cellular immune environment in endometrial polyps

The expression of estrogen receptors as well as GREB1, c-MYC, and cyclin D1, estrogen-regulated genes implicated in proliferation, is increased in peritoneal.

Expression of microtubule associated protein 2 and synaptophysin in endometrium: high levels in deep infiltrating endometriosis lesions Martina Gori,

Differential expression of vascular endothelial growth factor angiogenic factors in different endometrial compartments in women who have an elevated progesterone.

Increased expression of p21-activated kinase in adenomyosis

Regine Gaetje, M. D. , Uwe Holtrich, Ph. D. , Thomas Karn, Ph. D

Mast cells in human testicular biopsies from patients with mixed atrophy: increased numbers, heterogeneity, and expression of cyclooxygenase 2 and prostaglandin.

Suppression of migratory/invasive ability and induction of apoptosis in adenomyosis- derived mesenchymal stem cells by cyclooxygenase-2 inhibitors Yi-Jen.

Interleukin-18 system messenger RNA and protein expression in human endometrium during the menstrual cycle Hong-Yuan Huang, M.D., She-Hung Chan, B.Sc.,

Allergen-induced, eotaxin-rich, proangiogenic bone marrow progenitors: A blood-borne cellular envoy for lung eosinophilia Kewal Asosingh, PhD, Jodi D.

Physical exercise modulates the homeostasis of human regulatory T cells Max Weinhold, MA, Alexander Shimabukuro-Vornhagen, MD, Axel Franke, MD, Sebastian.

Increased matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-1 secretion but unaffected invasiveness of endometrial stromal cells in.

Prolonged gonadotropin-releasing hormone agonist therapy reduced expression of nitric oxide synthase in the endometrium of women with endometriosis and.

Immunohistochemical detection of aquaporin expression in eutopic and ectopic endometria from women with endometriomas Xiu-Xiu Jiang, M.D., Riu-Jin Wu,

Pathophysiologic features of “thin” endometrium

Xiao-Yu Pan, Ph. D. , Xue Li, M. D. , Zhan-Ping Weng, Ph. D

Influences on endometrial development during intrauterine insemination: clinical experience of 2,929 patients with unexplained infertility Erin Foran.

Expression of erythropoietin messenger ribonucleic acid in wild-type MED12 uterine leiomyomas under estrogenic influence: new insights into related growth.

HOXA-10 and E-cadherin expression in the endometrium of women with recurrent implantation failure and recurrent miscarriage Yihua Yang, Ph.D., Xiaoyan.

Interleukin-1β regulation of gonadotropin-releasing hormone messenger ribonucleic acid in cultured human endometrial stromal cells Hong-Yuan Huang, M.D.,

Immunohistochemical detection of heparanase-1 expression in eutopic and ectopic endometrium from women with endometriosis Xiulong Xu, Ph.D., Jianchi.

Up-regulation of endocrine gland-derived vascular endothelial growth factor but not vascular endothelial growth factor in human ectopic endometriotic.

Murat Ulukus, M. D. , E. Cagnur Ulukus, M. D. , Ege N

Excessive ovarian response is associated with increased expression of interleukin-2 in the periimplantation endometrium Guneet Makkar, Ph.D., Ernest.

Nina Chehna-Patel, M. Sc. , Geetanjali Sachdeva, Ph. D

Ching-wen Cheng, Ph. D. , Stephen K. Smith, M. D. , D

Macrophage migration inhibitory factor expression in the intrauterine endometrium of women with endometriosis varies with disease stage, infertility status,

Qiong-Hua Chen, Ph. D. , Wei-Dong Zhou, Ph. D. , De-Min Pu, M. D

Philip Kressin, M. D. , Eva-Maria Wolber, Ph. D. , Harald Wodrich, Ph

A.K.I. Kuroki, Masayuki Iyoda, Takanori Shibata, Tetsuzo Sugisaki

Fei Wang, M. D. , Ph. D. , Zeqing Wen, M. D. , Hui Li, Ph. D

Interleukin-1 system messenger ribonucleic acid and protein expression in human fallopian tube may be associated with ectopic pregnancy Hong-Yuan Huang,

Development and validation of a three-dimensional in vitro model for uterine leiomyoma and patient-matched myometrium Minnie Malik, Ph.D., William H.

Narendra P Singh, M. B. B. S. , M. S. , Charles H Muller, Ph. D

Human myometrium and leiomyomas express gonadotropin-releasing hormone 2 and gonadotropin-releasing hormone 2 receptor Jason D. Parker, D.O., Minnie.

Local injury of the endometrium induces an inflammatory response that promotes successful implantation Yulia Gnainsky, Ph.D., Irit Granot, Ph.D., Paulomi.

Lin Mu, Ph. D. , Wei Zheng, Ph. D. , M. D. , Liang Wang, Ph. D

Decreased levels of interleukin-18 in peritoneal fluid but not in serum of patients with endometriosis Xinmei Zhang, M.D., Jun Lin, M.D., Yuli Qian,

Higher PDCD4 expression is associated with obesity, insulin resistance, lipid metabolism disorders, and granulosa cell apoptosis in polycystic ovary syndrome

Effects of hyperglycemia on the differential expression of insulin and insulin-like growth factor-I receptors in human normal peritoneal and adhesion.

Microvascular density, vascular endothelial growth factor A, and its receptors in endometrial blood vessels in patients with menorrhagia Miriam Mints,

Fernando M Reis, M. D. , Ph. D. , Ana Luiza Maia, M. D. , Ph. D

Prokineticin 1 mRNA expression in the endometrium of healthy women and in the eutopic endometrium of women with endometriosis Federica Tiberi, B.S.,

Elevated levels of gremlin-1 in eutopic endometrium and peripheral serum in patients with endometriosis Guihua Sha, Ph.D., Yan Zhang, M.D., Chengyan.

Regulatory T cells, inflammation, and endoplasmic reticulum stress in women with defective endometrial receptivity Mario Galgani, Ph.D., Luigi Insabato,

Quantitative expression of apoptosis-regulating genes in endometrium from women with and without endometriosis Donald P. Braun, Ph.D., Jianchi Ding,

MicroRNA-22-3p is down-regulated in the plasma of Han Chinese patients with premature ovarian failure Yujie Dang, M.D., Ph.D., Shidou Zhao, Ph.D., Yingying.

Cyclooxygenase-2 selective inhibitor prevents implantation of eutopic endometrium to ectopic sites in rats Sachiko Matsuzaki, M.D., Michel Canis, M.D.,

Presentation transcript:

The disturbance of TH17-Treg cell balance in adenomyosis Tao Gui, M.D., Chen Chen, M.D., Zhenzhen Zhang, M.D., Weiwei Tang, M.D., Ruyun Qian, M.D., Xiaoping Ma, M.D., Peng Cao, Ph.D., Guiping Wan, Ph.D. Fertility and Sterility Volume 101, Issue 2, Pages 506-514 (February 2014) DOI: 10.1016/j.fertnstert.2013.10.050 Copyright © 2014 Terms and Conditions

Figure 1 (A) Representative flow cytometry results, showing identification of TH17 and Treg cells in peripheral blood from control women (n = 25), and patients with diffuse (D-AM; n = 29) and focal (F-AM; n = 16) adenomyosis. Analysis of the frequency of (B) TH17 cells, (C) Treg cells, and (D) TH17-Treg ratios in peripheral blood of control women (n = 25) and patients with D-AM (n = 29) and F-AM (n = 16). Dashed lines represent medians, solid lines the lower and upper quartiles. (E) Relative expression of ROR-γt, FOXP3, STAT3 mRNA in control, eutopic, and homologous ectopic endometrium. (F) The histogram shows median concentrations of interleukin (IL) 6, IL-10, IL-17, and transforming growth factor (TGF) β (pg/mL) in the plasma of patients from D-AM (n = 29), F-AM (n = 16), and control (n = 25) groups. Bars and whiskers indicate mean ± SD. Differences between groups were analyzed by Tukey multiple comparision test of one-way analysis of variance (ns = not significant; *P<.05; **P<.01; ***P<.01). Fertility and Sterility 2014 101, 506-514DOI: (10.1016/j.fertnstert.2013.10.050) Copyright © 2014 Terms and Conditions

Figure 2 (A) Scatter plot of TH17-Treg ratios and CA-125 levels in women with adenomyosis (AM). The red and blue symbols represent data points from women with diffuse and focal AM, respectively. Pearson rank correlation coefficient was used to analysis their correlations. The dashed lines indicate linear regression fits of the corresponding data. (B) Scatter plot depicting the relationship between the severity of dysmenorrhea in AM and the TH17-Treg ratio in peripheral blood mononuclear cells of patients with AM, in which the solid line represents the lower and upper quartiles and the dashed lines the medians. Fertility and Sterility 2014 101, 506-514DOI: (10.1016/j.fertnstert.2013.10.050) Copyright © 2014 Terms and Conditions

Figure 3 (A) Relative expression of FOXP3 and IL-17A mRNA determined by real-time polymerase chain reaction in control endometrium (n = 25), eutopic endometrium (n = 45), and homologous ectopic endometrium (n = 45). (B) Expression of IL-17A+ and FOXP3+ cells in normal endometrium and eutopic and homologous ectopic endometrium. (a) Negative control, using isotype antibodies (matched to IL-17A) staining in normal endometrial tissue. (b, c, d) Immunohistochemical staining of IL-17A in normal, eutopic, and ectopic endometrial samples, respectively. (e) Negative control, using isotype antibodies (matched to FOXP3) staining in adenomyotic lession. (f, g, h) Immunohistochemical staining of FOXP3 in normal, eutopic, and homologous ectopic endometrial samples, respectively. All magnification ×200. Scale bars = 100 μm. Histograms show (C) FOXP3+ and (D) IL-17A+ cell densities per mm2 within the normal, eutopic, and homologous ectopic endometrial samples. Fertility and Sterility 2014 101, 506-514DOI: (10.1016/j.fertnstert.2013.10.050) Copyright © 2014 Terms and Conditions