Antinociceptive potentiation and attenuation of tolerance by intrathecal β-arrestin 2 small interfering RNA in rats C.-H. Yang, H.-W. Huang, K.-H. Chen,

Slides:

Advertisements

Similar presentations

From: Amitriptyline Suppresses Neuroinflammation-dependent Interleukin-10-p38 Mitogen-activated Protein Kinase-Heme Oxygenase-1 Signaling Pathway in Chronic.

Advertisements

Activation of Src Family Kinases in Spinal Microglia Contributes to Formalin-Induced Persistent Pain State Through p38 Pathway Yong-Hui Tan, Kai Li,

A-LEVEL BIOLOGY RNA interference (RNAi)

Electroacupuncture inhibits excessive interferon-γ evoked up-regulation of P2X4 receptor in spinal microglia in a CCI rat model for neuropathic pain

A Signal Transduction Pathway from TGF-β1 to SKP2 via Akt1 and c-Myc and its Correlation with Progression in Human Melanoma Xuan Qu, Liangliang Shen,

An Opiate Cocktail that Reduces Morphine Tolerance and Dependence

IL-10 reduces apoptosis and extracellular matrix degradation after injurious compression of mature articular cartilage P. Behrendt, A. Preusse-Prange,

Glutamate signaling in chondrocytes and the potential involvement of NMDA receptors in cell proliferation and inflammatory gene expression T. Piepoli,

H. Wang, Y. Dong, J. Zhang, Z. Xu, G. Wang, C. A. Swain, Y. Zhang, Z

Development of Cell-Penetrating Asymmetric Interfering RNA Targeting Connective Tissue Growth Factor Jihye Hwang, Chanil Chang, Ji Hyun Kim, Chang Taek.

Role of hypoxia-inducible factor-1 alpha in the regulation of plasminogen activator activity in rat knee joint chondrocytes G. Zhu, Y. Tang, X. Liang,

Characterization of TNF-α– and IL-17A–Mediated Synergistic Induction of DEFB4 Gene Expression in Human Keratinocytes through IκBζ Claus Johansen, Trine.

Functional Investigation of Fas Ligand Expressions in Human Non-Small Cell Lung Cancer Cells and Its Clinical Implications Yidan Lin, MD, PhD, Lunxu.

NF-κBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1β-induced and TNF-α-induced chondrocytes Dr C. Lianxu, Ph.D.,

Huan Yang, Caixia Lei, Wei Zhang Reproductive BioMedicine Online

L. Raymond, S. Eck, E. Hays, I. Tomek, M. D. , S. Kantor, M. D. , M

Volume 16, Issue 1, Pages (January 2008)

Interferon-γ Protects from Staphylococcal Alpha Toxin-Induced Keratinocyte Death through Apolipoprotein L1 Anne M. Brauweiler, Elena Goleva, Donald Y.M.

Chronic intrathecal infusion of mibefradil, ethosuximide and nickel attenuates nerve ligation-induced pain in rats Y.L. Chen, M.L. Tsaur, S.W. Wang,

Short small-interfering RNAs produce interferon-α-mediated analgesia

S. Picardi, M. F. Stevens, K. Hahnenkamp, M. E. Durieux, P. Lirk, M. W

Mu and delta, but not kappa, opioid agonists induce spastic paraparesis after a short period of spinal cord ischaemia in rats† M Kakinohana, S Nakamura,

Α7 Nicotinic acetylcholine receptor agonist GTS-21 attenuates ventilator-induced tumour necrosis factor-α production and lung injury M. Kox, J.C. Pompe,

Ella H. Sklan, Jeffrey S. Glenn Gastroenterology

Morphine tolerance increases [3H]MK-801 binding affinity and constitutive neuronal nitric oxide synthase expression in rat spinal cord Chih-Shung Wong,

Leukemia inhibitory factor (LIF) potentiates antinociception activity and inhibits tolerance induction of opioids H.J. Tu, K.H. Kang, S.Y. Ho, H.C. Liou,

L. Tong, M. Cai, Y. Huang, H. Zhang, B. Su, Z. Li, H. Dong

G. Xu, H. Lu, Y. Dong, D. Shapoval, S. G. Soriano, X. Liu, Y. Zhang, Z

Role of vitamin C and selenium in attenuation of nicotine induced oxidative stress, P53 and Bcl2 expression in adult rat spleen Marwa A. Ahmed, K.H.

General anaesthetics do not impair developmental expression of the KCC2 potassium- chloride cotransporter in neonatal rats during the brain growth spurt

DIO2 modifies inflammatory responses in chondrocytes

J.-Y. Hwang, H.-S. Na, Y.-T. Jeon, Y.-J. Ro, C.-S. Kim, S.-H. Do

Selective Inhibition of p300 HAT Blocks Cell Cycle Progression, Induces Cellular Senescence, and Inhibits the DNA Damage Response in Melanoma Cells Gai.

Annexin 1 exerts anti-nociceptive effects after peripheral inflammatory pain through formyl-peptide-receptor-like 1 in rat dorsal root ganglion L. Pei,

Single sevoflurane exposure decreases neuronal nitric oxide synthase levels in the hippocampus of developing rats X. Feng, J.J. Liu, X. Zhou, F.H. Song,

SB366791, a TRPV1 antagonist, potentiates analgesic effects of systemic morphine in a murine model of bone cancer pain† Y. Niiyama, T. Kawamata, J. Yamamoto,

Antinociceptive role of 5-HT1A receptors in rat spinal cord

Intrathecal cyclooxygenase inhibitor administration attenuates morphine antinociceptive tolerance in rats C. -S. Wong, M. -M. Hsu, R. Chou, Y. -Y. Chou,

Amadeus S. Zhu, Ang Li, Tabetha S. Ratliff, Martha Melsom, Luis A

Volume 133, Issue 4, Pages (October 2007)

L. -C. Chu, M. -L. Tsaur, C. -S. Lin, Y. -C. Hung, T. -Y. Wang, C. -C

Glutamate signaling in chondrocytes and the potential involvement of NMDA receptors in cell proliferation and inflammatory gene expression T. Piepoli,

Lidocaine time- and dose-dependently demethylates deoxyribonucleic acid in breast cancer cell lines in vitro† P. Lirk, R. Berger, M.W. Hollmann, H. Fiegl

Molecular Therapy - Nucleic Acids

Volume 57, Issue 5, Pages (May 2000)

Volume 22, Issue 5, Pages (May 2014)

Regulation of mechanical stress-induced MMP-13 and ADAMTS-5 expression by RUNX-2 transcriptional factor in SW1353 chondrocyte-like cells T. Tetsunaga,

Knocking down Disease with siRNAs

Pharmacodynamic changes with vecuronium in sepsis are associated with expression of α7- and γ-nicotinic acetylcholine receptor in an experimental rat.

V Minville, O Fourcade, J.-P. Girolami, I Tack

The novel Kv1.5 channel blocker vernakalant for successful treatment of new-onset atrial fibrillation in a critically ill abdominal surgical patient

R.D. Sanders, N Patel, M Hossain, D Ma, M Maze

Volume 10, Issue 2, Pages (January 2015)

Autoantigen La Promotes Efficient RNAi, Antiviral Response, and Transposon Silencing by Facilitating Multiple-Turnover RISC Catalysis Ying Liu, Huiling.

P. Ecimovic, D. Murray, P. Doran, J. McDonald, D. G. Lambert, D. J

Jens Gaedeke, Nancy A. Noble, Wayne A. Border Kidney International

The Membrane-Lytic Peptides K8L9 and Melittin Enter Cancer Cells via Receptor Endocytosis following Subcytotoxic Exposure Masayuki Kohno, Tomohisa Horibe,

Inflammation-induced up-regulation of ionotropic glutamate receptor expression in human skin P.-H. Tan, L.-C. Yang, P.-T. Chiang, J.S.-C. Jang, H.-C.

Alleviation of neuropathic pain by intrathecal injection of antisense oligonucleotides to p65 subunit of NF-κB T. Sun, W.-G. Song, Z.-J. Fu, Z.-H. Liu,

Effects of capsazepine, a transient receptor potential vanilloid type 1 antagonist, on morphine-induced antinociception, tolerance, and dependence in.

ICU fire evacuation preparedness in London: a cross-sectional study

Suspected opioid-induced hyperalgesia in an infant

Cross-tolerance between spinal neostigmine and morphine in the rat

Volume 66, Issue 3, Pages (March 2017)

Molecular Therapy - Nucleic Acids

C.M. Chalmers, A.M. Bal British Journal of Anaesthesia

M. J. Sheen, S. -T. Ho, C. -H. Lee, Y. -C. Tsung, F. -L. Chang, S. -T

Volume 131, Issue 5, Pages (November 2006)

Antinociceptive effect of intrathecal ginsenosides through α-2 adrenoceptors in the formalin test of rats M.H. Yoon, L.J. Huang, J.I. Choi, H.G. Lee,

Rumwald Leo G Lecaros, Leaf Huang, Tsai-Chia Lee, Yih-Chih Hsu

Presentation transcript:

Antinociceptive potentiation and attenuation of tolerance by intrathecal β-arrestin 2 small interfering RNA in rats C.-H. Yang, H.-W. Huang, K.-H. Chen, Y.-S. Chen, S.-M. Sheen-Chen, C.-R. Lin British Journal of Anaesthesia Volume 107, Issue 5, Pages 774-781 (November 2011) DOI: 10.1093/bja/aer291 Copyright © 2011 The Author(s) Terms and Conditions

Fig 1 (a) Experimental design. (IT, intrathecal.) Male Sprague–Dawley rats received intrathecal injections of i-Fect encapsulated β-arrestin 2 siRNA, non-targeting dsRNA, the transfection reagent (i-Fect), or normal saline once daily, for 3 days. After pretreatment, the rats received continuous intrathecal (osmotic minipump) saline or morphine (2 nmol h−1) infusions for 7 days. Five animals were included in each treatment group. (b) The mechanism of β-arrestin 2 siRNA processing. After cellular uptake of synthetic β-arrestin 2 siRNA, one of the two siRNA strands is cleaved and released, whereas the remaining single strand binds to and activates the RNA-induced silencing complex (RISC). The single-strand siRNA together with the activated RISC binds to its complementary β-arrestin 2 mRNA. The RISC contains an enzyme to cleave β-arrestin 2 mRNA and therefore cause gene knock down. British Journal of Anaesthesia 2011 107, 774-781DOI: (10.1093/bja/aer291) Copyright © 2011 The Author(s) Terms and Conditions

Fig 2 Real-time RT–PCR and western blot analysis showing a decrease in β-arrestin 2 but not β-arrestin 1 mRNA and protein level in the spinal cord after intrathecal injection with β-arrestin 2 siRNA. β-arrestin 1 and 2 mRNA and protein level in control and β-arrestin 2 siRNA-injected rats were monitored by qRT–PCR over 9 days after injection. Each kinetic point was performed in triplicate on three to seven pooled rats. For each sample, β-arrestin 1 and 2 transcripts or protein level was normalized against GAPDH. Normalized β-arrestin 1 and 2 expression in β-arrestin 2 siRNA-injected rats was expressed as the proportion of the expression recorded in the control. Average percentages of mRNA depletion are indicated. Data are presented as mean (sd), *P<0.05. British Journal of Anaesthesia 2011 107, 774-781DOI: (10.1093/bja/aer291) Copyright © 2011 The Author(s) Terms and Conditions

Fig 3 (a) Thermal TF latencies in rats receiving intrathecal injection with β-arrestin 2 siRNA for 3 days. Significant difference of prolonged latency was noted in rats receiving β-arrestin 2 siRNA (b). Time course of the changes in thermal antinociception and %MPE as measured by the TF test in rats receiving continuous intrathecal infusions. Data are presented as means (sd) (n=10). See the text for details of treatment groups. *Different from morphine 2 nmol h−1 on that day, P<0.05. +Different from day 2 value within the group, P<0.05. British Journal of Anaesthesia 2011 107, 774-781DOI: (10.1093/bja/aer291) Copyright © 2011 The Author(s) Terms and Conditions

Fig 4 Effect of β-arrestin 2 siRNA treatment on the occurrence of withdrawal signs in response to naloxone in (a) saline-infused rats, or (b) morphine-infused rats. a, vocalization in response to light touch with a piece of polyethylene tubing; b, spontaneous vocalization; c, abnormal posture; d, ejaculation; e, ‘wet dog head shakes’; f, escape attempts. Each group contained eight rats. *Different from morphine 2 nmol h−1 on that day, P<0.05. British Journal of Anaesthesia 2011 107, 774-781DOI: (10.1093/bja/aer291) Copyright © 2011 The Author(s) Terms and Conditions

Fig 5 Tail withdrawal latency and %MPE to an intrathecal probe morphine bolus dose administered on day 7 after continuous intrathecal drug infusion. Each point represents mean (sd) (n=6). *P<0.05 compared with saline-treated (rats receiving morphine 0 nmol h−1 infusion). +P<0.05 compared with saline-treated (rats receiving morphine 2 nmol h−1 infusion). British Journal of Anaesthesia 2011 107, 774-781DOI: (10.1093/bja/aer291) Copyright © 2011 The Author(s) Terms and Conditions