Genome-wide identification of aberrantly methylated promoters in ovarian tissue of prenatally androgenized rats Duojia Zhang, M.D., Ph.D., Jing Cong,

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Genome-wide identification of aberrantly methylated promoters in ovarian tissue of prenatally androgenized rats Duojia Zhang, M.D., Ph.D., Jing Cong, Ph.D., Huanhuan Shen, M.M., Qi Wu, M.D., Xiaoke Wu, M.D., Ph.D. Fertility and Sterility Volume 102, Issue 5, Pages 1458-1467 (November 2014) DOI: 10.1016/j.fertnstert.2014.07.1203 Copyright © 2014 Terms and Conditions

Figure 1 (A–D) Hematoxylin and eosin staining of rat ovaries. (A, B) Ovary tissue in control groups, which had more lutea and follicles of various developmental periods. (C, D) Ovary tissue in PNA groups. There were a lot of cystic follicles and atresic follicles. (E–G) Quantification of immunohistochemical assay was represented as percentage of active caspase-3 positively stained cells. Data shown are averages with SD from six individual rats in each group. ∗P<.01 vs. corresponding basal values. (E) Control group; (F) PNA group; (H–J) TUNEL assay for in situ detection of apoptosis; (H) control group; (I) PNA group. ∗P<.01 vs. corresponding basal values. Fertility and Sterility 2014 102, 1458-1467DOI: (10.1016/j.fertnstert.2014.07.1203) Copyright © 2014 Terms and Conditions

Figure 2 (A) GO categories of the hypermethylated candidate genes obtained from the MeDIP-chip approach. Ontology terms (Y-axis). P-value analyzed by Fisher exact test under log processing (X-axis). (B) Validation of real-time PCR of the enriched DNAs obtained from the MeDIP assays. Data are means ± SEM.*P<.01 vs. controls. (C) Quantitative real-time reverse transcriptase–PCR analysis of Bcl2l1 mRNA relative to GAPDH. Fertility and Sterility 2014 102, 1458-1467DOI: (10.1016/j.fertnstert.2014.07.1203) Copyright © 2014 Terms and Conditions