The Ins and Outs of the Rice AGAMOUS Subfamily Ludovico Dreni, Michela Osnato, Martin M. Kater  Molecular Plant  Volume 6, Issue 3, Pages 650-664 (May 2013) DOI: 10.1093/mp/sst019 Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

Figure 1 Arabidopsis and Rice Flowers, Wild-Type, and AGAMOUS Subfamily Mutants. (A, B) Wild-type and ag-3 mutant of Arabidopsis, respectively. CRice wild-type flower at anthesis. DRice wild-type flower, sterile glumes, lemma, and palea have been removed to show the three inner floral whorls (lodicules, stamens, and carpel). EThe inner whorls of a rice osmads3 osmads58 double mutant, showing the complete loss of sexual organ identity and floral meristem determinacy. FA rice wild-type pistil. (G, H) Intermediate and strong phenotypes, respectively, of the rice osmads13 mutant. The white star indicates the position of the second-whorl lodicules; white arrowheads and arrows indicate rudimentary glumes and sterile glumes, respectively; red arrowheads mark the ectopic palea-like organs replacing carpels in the osmads3 osmads58 double mutant; l, lemma; p, palea. Bars represent 0.5 mm. Molecular Plant 2013 6, 650-664DOI: (10.1093/mp/sst019) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

Figure 2 Phylogenetic Tree of AGAMOUS Subfamily Proteins. The dichotomy between AGAMOUS and AGL11 lineages is marked with a black star. Sequences which have not been used in previous phylogenetic studies (Kramer et al., 2004; Zahn et al., 2006; Dreni et al., 2007; Ciaffi et al., 2011) belong to sorghum, foxtail millet, Brachypodium, Oryza brachyantha, banana, and date palm, and are marked with a black arrowhead. The four grass subclades are also indicated. Sequences have been recovered from Phytozome, NCBI, CIRAD, Gramene, and the website of the Weill Cornell Medical College in Qatar. Provisional names were assigned to these sequences; the corresponding loci on the current genomic sequence releases are listed in Supplemental Table 1. Incomplete and incorrect genomic annotations were, if necessary, adjusted manually and by comparison with related proteins using Tblasttn (NCBI). The protein sequences were aligned using T-Coffee and analyzed with the Neighbour-Joining method using the Phylip Package, with 100 replicates. Am, Antirrhinum majus; Ap, Agapanthus praecox; At, Arabidopsis thaliana; Av, Asparagus virgatus; Bd, Brachypodium distachyon; Ce, Cycas edentata (outgroup); Dc, Dendrobium crumenatum; Ec, Eschscholzia californica; Gg, Gnetum gnemon; Ho, Hyacinthus orientalis; Ll, Lilium longiflorum; Mp, Magnolia praecocissima; Ms, Magnolia stellata; Ma, Musa acuminata; No, Nymphaea odorata; Ob, Oryza brachyantha; Os, Oryza sativa; Ph, Petunia x hybrida; Pd, Phoenix dactylifera; Rf, Ranunculus ficaria/Ficaria verna; Si, Setaria italica; Sb, Sorghum bicolor; Ta, Triticum aestivum; Zm, Zea mays. Molecular Plant 2013 6, 650-664DOI: (10.1093/mp/sst019) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

Figure 3 Alignment of the Five Rice AGAMOUS Subfamily Proteins. The putative N-termini of OsMADS3, OsMADS58, and OsMADS66 are included. The MADS and K domains and the three alfa helices are also indicated. Molecular Plant 2013 6, 650-664DOI: (10.1093/mp/sst019) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

Figure 4 SEM Images Showing the Early Stages of Development of the Rice Gynoecium in the Fourth Whorl. AFive of the six stamen primordia and the undifferentiated floral meristem are visible. BThe three fused carpel primordia (arrowheads) start to enclose the ovule primordium. CThe ovary wall almost completely enveloped the ovule primordium, and the two lateral carpel primordia are differentiating the stigmas (arrowheads). Bars represent 25 µm. Molecular Plant 2013 6, 650-664DOI: (10.1093/mp/sst019) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions