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Volume 114, Issue 1, Pages 115-122 (January 1998) CD4+ T cells with specific reactivity against astrovirus isolated from normal human small intestine Øyvind Molberg, Ellen M. Nilsen, Ludvig M. Sollid, Helge Scott, Per Brandtzaeg, Erik Thorsby, Knut E.A. Lundin Gastroenterology Volume 114, Issue 1, Pages 115-122 (January 1998) DOI: 10.1016/S0016-5085(98)70639-0 Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 1 iHAst-specific proliferative responses in PBMCs from 29 healthy adult subjects. PPD from M. tuberculosis was used as a positive control antigen and noninfected Caco-2 cells as a negative control antigen. Proliferative responses were measured by [3H]thymidine uptake for the last 20 hours of a 7-day antigenic stimulation. Rate of proliferation is given as SI (Antigen-Specific Proliferation of PBMCs ÷ PBMC Proliferation Without Antigen Stimulation). Horizontal lines indicate mean value of SI for each antigen. Gastroenterology 1998 114, 115-122DOI: (10.1016/S0016-5085(98)70639-0) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 2 iHAst-specific proliferative responses and HLA restriction of small intestinal TCLs from 4 adult subjects. Responses were measured by [3H]thymidine uptake 48-72 hours after iHAst stimulation. Autologous PBMCs were used as APCs. Inhibition of iHAst-specific responses was performed by the addition of anti-HLA MAbs (10 mg/mL) 30 minutes before addition of the TCL. Data for each T-cell line are from one representative experiment (out of 3). Gastroenterology 1998 114, 115-122DOI: (10.1016/S0016-5085(98)70639-0) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 3 HLA restriction pattern of 6 iHAst-specific CD4+ TCCs derived from duodenal biopsy specimens from 2 adult subjects (As3, HLA-DR2,DQ6/DR4,DQ7; and As5, HLA-DR2,DQ6/DR8,DQ4). As APCs, autologous PBMCs and allogeneic HLA-typed PBMCs were used. Responses were measured by [3H]thymidine uptake 48-72 hours after iHAst stimulation. Inhibition of iHAst-specific responses was performed by addition of anti-HLA MAbs (10 mg/mL) 30 minutes before addition of TCC. Data given on each TCC are from one representative experiment (out of 2). Gastroenterology 1998 114, 115-122DOI: (10.1016/S0016-5085(98)70639-0) Copyright © 1998 American Gastroenterological Association Terms and Conditions