Engineering the End: DNA Processing at Human Telomeres

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Engineering the End: DNA Processing at Human Telomeres Carolyn Price, Naduparambil K. Jacob Molecular Cell Volume 18, Issue 2, Pages 147-148 (April 2005) DOI: 10.1016/j.molcel.2005.03.024 Copyright © 2005 Elsevier Inc. Terms and Conditions

Figure 1 Techniques Used to Analyze the G and C Strand Termini of Human Telomeres (A) Organization of the G strand overhang. (B) C strand analysis. Adaptor oligonucleotides are hybridized to the G strand overhang; only those oligonucleotides that hybridize directly adjacent to the 5′ end will be ligated and amplified by PCR. (C) G strand analysis. A 60 nt guide oligo is hybridized to the G overhang. This serves as a platform for hybridization and subsequent ligation of adaptor oligonucleotides. Black lines represent the chromosome terminus; blue bars, nontelomeric segment of adaptor oligonucleotide; red arrows, PCR primers Molecular Cell 2005 18, 147-148DOI: (10.1016/j.molcel.2005.03.024) Copyright © 2005 Elsevier Inc. Terms and Conditions