GENESDEV/2009/125252_Wu_ Supplemental Fig1

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GENESDEV/2009/125252_Wu_ Supplemental Fig1 1.5 1 Heme Level * * 0.5 SA (mM): 0 1 2.5 5 10

GENESDEV/2009/125252_Wu_ Supplemental Fig2 SiRev-erb - + Rev-erb GSK3

GENESDEV/2009/125252_Wu_ Supplemental Fig3 PCDNA3.1 Rev-eb * 2 Fold Repression 1 Reporter: hPGC1-luc Rev-erb: B 2 PCDNA3.1 Rev-eb * Fold Repression 1 Rev-erb hPGC1-luc: WT RREpm RREdm RREp/dm

GENESDEV/2009/125252_Wu_ Supplemental Fig4 2.0 SiControl SiRev-eb 1.5 1.0 ChIP enrichment 0.5 Ab: HDAC3 Hsp90

GENESDEV/2009/125252_Wu_ Supplemental Fig5 shRNA - + HDAC3 b-actin B siRNA - + NCoR b-actin

GENESDEV/2009/125252_Wu_ Supplemental Fig6 3 * 2 PGC1 mRNA 1 Succinylacetone(mM):0 1 2.5 5 B 5 mRNA level DMSO 4 SA+DMSO SA+Heme 3 * * 2 1 PGC1 Bmal1 C Dex 1uM ETOH + + + + + Heme PGC1a Rev-erba b-actin

Oxygen Consumption Rate GENESDEV/2009/125252_Wu_ Supplemental Fig7 Control-3T3 Cells 1.5 1.0 Oxygen Consumption Rate Respiration-driven 0.5 DMSO Hemin (2.5M) 7

GENESDEV/2009/125252_Wu_ Supplemental Fig8 1.5 Rev-erb mRNA level 1.0 0.5 Day1 Day3 Day5

* * * * GENESDEV/2009/125252_Wu_ Supplemental Fig9 A B S (%) 10 8 S (%) * 6 * 4 2 Control-3T3 REV-3T31 REV-3T32 B 100 80 * * 60 G1(%) 40 20 Control-3T3 REV-3T31 REV-3T32