GENESDEV/2009/125252_Wu_ Supplemental Fig1

Slides:



Advertisements
Similar presentations
Supplemental Figure 1 a % pulldown from input LNCaP/scrambled-siRNA Figure 1 A, ChIP analysis of AR and PHB binding to the PSA promoter in the LNCaP/scrambled-siRNA.
Advertisements

Expression of Nuclear Receptors during Adipogenesis of 3T3-L1 Cells COUP-TFI COUP-TFII PPAR  VDR GCNF ROR  HNF4  LXR  LXR  RXR  Nur77 REV-ERB  REV-ERB.
Control siRNA BRG1 siRNA PTEN – Akt – p-Akt – p-GSK-3  – Cyclin D1 – BRG1 –  -actin – SW480 Supplemental data S1 Supplemental data S1. Effects of transduction.
AB 11 22 33 44 55 66 77 88 99 10  20  19  18  17  16  15  14  13  12  11  21  22  23  24  25  26  27  28.
Knight et al – Supplementary Table Supplemental Table 1: Cell line Densitometry AROSSIRT1 ARPE WI MCF10A HCT HCT116.
Figure 1.1 The observer in the truck sees the ball move in a vertical path when thrown upward. (b) The Earth observer views the path of the ball as a parabola.
H1299 Vector H1648 TUSC2 TUSC2 -ER 2.3 uM ER H1299 Vector TUSC2 TUSC2 -ER 2.3 uM ER H1299 Vector H1648 TUSC2 TUSC2 -ER 2.3 uM ER H1299 Vector TUSC2 TUSC2.
(+++) Normal breast ATM (++) IDC ATM Lymph node metastasis negative positive P-value A B X200 C Vector ATM - WT.
Condensed TUNEL + cells (%) WT RanBP9 Tg B The scale bar represents 50  m A CA1 TUNEL Wild TypeRanBP9 Tg P
Supplemental data Fig. S1 Figure S1 Circulation bilirubin levels in CRC patients and non-tumor patients. Circulation bilirubin levels in CRC patients were.
Supplemental Figure 1: ER  expression in MCF-7 cells. MCF-7 cells were either pre-treated with ICI 182,780 (lanes 5-8) or EtOH (vehicle, lanes 1-4) for.
1A IP/ Input Figure 1: E47 is present on tRNA genes 1B IgG TFIIB E47 Brf1 WB: Brf1 Input IP A) IP/Input enrichment of E47 at 6 active tRNA genes and one.
Supplemental Table 1 Source and authentication of cell lines.
Supplemental Figure S1 ResidueMass Delta Mass Sequence Mascot Ion Score R.DFGLLVFVR.K K.IPALDLLIK.L K.FYGELALK.K34.
Nardella et al. Supplementary Fig. 1 Murine Rheb wt Line 47 Line 50 Log gene expression (a.u.) ******* 200 bp Founders
The reading is 7.38 mm. The reading is 7.72 mm.
EglN2 associates with the NRF1 ‐ PGC1α complex and controls mitochondrial function in breast cancer by Jing Zhang, Chengyang Wang, Xi Chen, Mamoru Takada,
Figure S1 HA-FOXO3 wt C terminus ab HA-FOXO3 wt HA ab untransfected C terminus ab untransfected HA ab
a c b S1. Lee et al., 2011 * RRT-binding PXDLS-binding NAD(H)-binding
Supplementary Fig. 1 *** Cell viability (%) HepG2 3 (hr) DU145 A549 17
Per2 Bmal1 Per3 Cry2 Cry1 Clock Ror CSNK1 Per1 Rev-erb
PTEN differentially regulates expressions of ICAM-1 and VCAM-1 through PI3K/Akt/GSK-3β/GATA-6 signaling pathways in TNF-α-activated human endothelial.
Progesterone receptor
Figure S1. qPCR analysis of the EMT markers ZEB2 and SNAI1 in Syndecan-1 and control siRNA transfected MDA-MB-231 and MCF-7 cells reveals no significant.
Supplemental Digital Content 4
Supplementary Figure S1.
A. B. C. D E. F. 100 GSK3β pGSK3β pAkt Akt p38/ SAPK
Vrtn functions as a transcriptional repressor.
Online Gifts Buy for wishes happy mother's day to yours choice and with happy gifts find here:
A B C D Supplementary Figure 1 CMV p300
Supplemental Figures Supplemental Figure 1.
Volume 17, Issue 9, Pages (November 2016)
REV-ERBα agonists reduce protein O-GlcNAcylation.
Matrix Mineralization
Supplemental Figure S1 a b
Volume 11, Issue 5, Pages (May 2007)
BET inhibition and depletion repress the expression of BRCA1 and RAD51
Supplemental Figure S2: G2 arrest in MCF7-shPBK1 stable cell line
PPARδ Promotes Running Endurance by Preserving Glucose
JQ1 directly represses the promoter activities of BRCA1 and RAD51
The anti-inflammatory effect of glucocorticoids is mediated by glucocorticoid-induced leucine zipper in epithelial cells  Jane Eddleston, PhD, Jack Herschbach,
A B CDK2 CDK2 inhibition P Activated KRAS P CP110 CP110
Relative mRNA expression level (/J3T-1)
C-MYC induces the transcription of LIG3 and PARP1 in FLT3/ITD- and BCR-ABL1–positive cells. c-MYC induces the transcription of LIG3 and PARP1 in FLT3/ITD-
SUMO Promotes HDAC-Mediated Transcriptional Repression
A Circadian Clock in HaCaT Keratinocytes
Relative amount of nuclear β-catenin
(A) Genotyping of the Lats2 and Cre alleles in the PyMT-derived cell lines (see primers location in Fig S2A). (A) Genotyping of the Lats2 and Cre alleles.
Anti-tumor effect of anti-PD-1 antibody against SCC3 tumor
A Novel Role of Transforming Growth Factor β1 in Transcriptional Repression of Human Cholesterol 7α-Hydroxylase Gene  Tiangang Li, John Y.L. Chiang  Gastroenterology 
Supplementary Fig. S1 BCL2 5’flanking ctrl siRNA luc
N H N H Figure S1 parp-1 +/+ parp-1 -/- HIF-2 DAPI
Volume 17, Issue 9, Pages (November 2016)
Reh cells, 24 hours c-Myc Ac-p53 b-actin
Volume 25, Issue 1, Pages (January 2017)
Nonsense-Mediated RNA Decay Influences Human Embryonic Stem Cell Fate
20S proteasome activities
HDAC11 regulates CCL2 expression by recruiting PU.1.
Circadian Dysfunction Induces Leptin Resistance in Mice
Class II HDACs are functionally redundant, but HDAC5 is the primary GLUT4 regulator in 3T3-L1 preadipocytes. Class II HDACs are functionally redundant,
Cell cycle release after M phase arrest (h)
Fig. 4. Effects of natriuretic peptide receptor 2 (NPR2) knockdown on cells apoptosis in mouse Leydig cells. (A) Relative mRNA expression of cell apoptosis.
Volume 2, Issue 3, Pages (September 2012)
SATB2-AS1 repressed Snail transcription depending on SATB2-mediated recruitment of HDAC1. SATB2-AS1 repressed Snail transcription depending on SATB2-mediated.
Pirh2 represses p73-dependent transactivation.
RPL26 is a positive regulator of TP53β splicing.
Validation of KMT2D function in MM23, MM2, and MM25 PDXs
A B C Name Sequence TIMP3 promoter
JQ1 selectively ablates PAX3–FOXO1-driven transcription and BRD4 interaction. JQ1 selectively ablates PAX3–FOXO1-driven transcription and BRD4 interaction.
CREB1 binds at the proximal region of the TGFB2 promoter and induces its transcriptional activation. CREB1 binds at the proximal region of the TGFB2 promoter.
Presentation transcript:

GENESDEV/2009/125252_Wu_ Supplemental Fig1 1.5 1 Heme Level * * 0.5 SA (mM): 0 1 2.5 5 10

GENESDEV/2009/125252_Wu_ Supplemental Fig2 SiRev-erb - + Rev-erb GSK3

GENESDEV/2009/125252_Wu_ Supplemental Fig3 PCDNA3.1 Rev-eb * 2 Fold Repression 1 Reporter: hPGC1-luc Rev-erb: B 2 PCDNA3.1 Rev-eb * Fold Repression 1 Rev-erb hPGC1-luc: WT RREpm RREdm RREp/dm

GENESDEV/2009/125252_Wu_ Supplemental Fig4 2.0 SiControl SiRev-eb 1.5 1.0 ChIP enrichment 0.5 Ab: HDAC3 Hsp90

GENESDEV/2009/125252_Wu_ Supplemental Fig5 shRNA - + HDAC3 b-actin B siRNA - + NCoR b-actin

GENESDEV/2009/125252_Wu_ Supplemental Fig6 3 * 2 PGC1 mRNA 1 Succinylacetone(mM):0 1 2.5 5 B 5 mRNA level DMSO 4 SA+DMSO SA+Heme 3 * * 2 1 PGC1 Bmal1 C Dex 1uM ETOH + + + + + Heme PGC1a Rev-erba b-actin

Oxygen Consumption Rate GENESDEV/2009/125252_Wu_ Supplemental Fig7 Control-3T3 Cells 1.5 1.0 Oxygen Consumption Rate Respiration-driven 0.5 DMSO Hemin (2.5M) 7

GENESDEV/2009/125252_Wu_ Supplemental Fig8 1.5 Rev-erb mRNA level 1.0 0.5 Day1 Day3 Day5

* * * * GENESDEV/2009/125252_Wu_ Supplemental Fig9 A B S (%) 10 8 S (%) * 6 * 4 2 Control-3T3 REV-3T31 REV-3T32 B 100 80 * * 60 G1(%) 40 20 Control-3T3 REV-3T31 REV-3T32