Sperm involved in recurrent partial hydatidiform moles cannot induce the normal pattern of calcium oscillations  Dimitra Nikiforaki, M.Sc., Frauke Vanden.

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Sperm involved in recurrent partial hydatidiform moles cannot induce the normal pattern of calcium oscillations  Dimitra Nikiforaki, M.Sc., Frauke Vanden Meerschaut, M.D., Ph.D., Stefanie De Gheselle, M.Sc., Chen Qian, M.D., Etienne Van den Abbeel, Ph.D., Winnok Harald De Vos, Ph.D., Tom Deroo, Ph.D., Petra De Sutter, M.D., Ph.D., Björn Heindryckx, Ph.D.  Fertility and Sterility  Volume 102, Issue 2, Pages 581-588.e1 (August 2014) DOI: 10.1016/j.fertnstert.2014.05.004 Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 1 (A) A single PHM sperm is unable to trigger normal Ca2+ oscillations in human vitro–matured oocytes, whereas (B) injecting two PHM sperm can provoke Ca2+ responses in some oocytes. Fertility and Sterility 2014 102, 581-588.e1DOI: (10.1016/j.fertnstert.2014.05.004) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 Immunostaining of failed fertilized oocytes after ICSI-AOA. Confocal reconstructions are shown for DNA (A, B, C, and D) and microtubules (E, F, G, and H). The oocytes exhibited a single PB with DNA and microtubules (white asterisk) and spindles (yellow arrow). The sperm heads (white arrow) were either decondensing (A, C) or fully decondensed (B). Fertility and Sterility 2014 102, 581-588.e1DOI: (10.1016/j.fertnstert.2014.05.004) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

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