Volume 56, Issue 3, Pages 1120-1128 (September 1999) GBV-C/HGV-RNA in serum and peripheral blood mononuclear cells in hemodialysis patients  María Cabrerizo, Javier Bartolomé, Patricia De Sequera, Carlos Caramelo, M. Luisa Manzano, Vicente Carreño  Kidney International  Volume 56, Issue 3, Pages 1120-1128 (September 1999) DOI: 10.1046/j.1523-1755.1999.00628.x Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 1 Hepatitis G virus (GBV-C/HGV)-RNA amplification using primers from the 5′-noncoding region (322 base pairs), from serum (S), PBMC (P), last phosphate buffered saline (PBS) wash (W), and serum after ultracentrifugation (S') from four hemodialysis patients: patient 1, with GBV-C/HGV-RNA in serum and peripheral blood mononuclear cells (PBMCs); patient 2, without GBV-C/HGV-RNA; patient 3, with GBV-C/HGV-RNA in PBMCs and in serum after ultracentrifugation; and patient 4, with GBV-C/HGV-RNA only in PBMCs. M, 100 base pair DNA ladder (GIBCO/BRL). Kidney International 1999 56, 1120-1128DOI: (10.1046/j.1523-1755.1999.00628.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 2 Comparison of nucleotide sequences of 240 bp of the amplified region of the GBV-C/HGV-RNA from serum after ultracentrifugation (S) and PBMC (P) samples from five hemodialysis (HD) patients: HD6, HD9, HD12, HD15, and HD17. The asterisks indicate 100% homology between all GBV-C/HGV sequences. Kidney International 1999 56, 1120-1128DOI: (10.1046/j.1523-1755.1999.00628.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 3 Unrooted phylogenetic tree of the GBV-C/HGV sequences isolated from serum after ultracentrifugation (S) samples from 20 hemodialysis (HD) patients. The bootstrap values were not statistically significant in any case. Kidney International 1999 56, 1120-1128DOI: (10.1046/j.1523-1755.1999.00628.x) Copyright © 1999 International Society of Nephrology Terms and Conditions