Characterization of the Different BCR-ABL Transcripts with a Single Multiplex RT-PCR Jacques Chasseriau, Jérôme Rivet, Frédéric Bilan, Jean-Claude Chomel,

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Characterization of the Different BCR-ABL Transcripts with a Single Multiplex RT-PCR Jacques Chasseriau, Jérôme Rivet, Frédéric Bilan, Jean-Claude Chomel, François Guilhot, Nicolas Bourmeyster, Alain Kitzis The Journal of Molecular Diagnostics Volume 6, Issue 4, Pages 343-347 (November 2004) DOI: 10.1016/S1525-1578(10)60530-2 Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Molecular structure of BCR and c-ABL genes and their main translocated transcripts. Arrows indicate breakpoints in BCR and c-ABL. Asterisks show sense primers which are used for the detection of different transcripts (e1, Tet; b1, Fam; e19, Hex). The reverse primer is systematically a3. The Journal of Molecular Diagnostics 2004 6, 343-347DOI: (10.1016/S1525-1578(10)60530-2) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Analysis of BCR-ABL transcripts in five different patients. The bottom panel of each result is the GS 500 size standard labeled with Tamara (75, 100, 139, 150, 160, 200, 250, 300, 340, 350, 400, 450, 490, and approximately 500 bases). The top panel shows the fluorescent multiplex electropherogram of each sample analyzed. For each sample, we indicate the color, the band size of the predominant amplicon in bases, and the corresponding transcript. The Journal of Molecular Diagnostics 2004 6, 343-347DOI: (10.1016/S1525-1578(10)60530-2) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions