Bsc (Hons) Biomedical Sciences, UKM PSEUDOMONAS spp. Lecture 12 Prepared by: Miss Norzawani Jaffar, Bsc (Hons) Biomedical Sciences, UKM

Learning Outcomes At the end of learning session, student must be able to: Describe characteristic of Pseudomonas sp. Explain the morphology and classification of Pseudomonas sp. Describe the laboratory test that significant for Pseudomonas sp identification. Explain the related disease and infections that potentially cause by Pseudomonas sp.

Pseudomonas sp Scientific classification Domain: Bacteria Phylum: Proteobacteria Class: Gammaproteobacteria Order: Pseudomonadales Family: Pseudomonadaceae Genus: Pseudomonas

Pseudomonas sp Strict aerobes widely distributed in nature They are mainly opportunist pathogens infecting those with impaired septicaemia. Some rare GNRs are environmental organism causing hospital-acquired infections from contaminated fluids or equipment. Other rare Gram negative rods are normal or animal flora causing opportunistic infections including dental infections, infected bites and systemic infections. Pseudomonal species have been found in soil, water, plants, and animals; Pseudomonas aeruginosa colonization reportedly occurs in more than 50% of humans, and P aeruginosa is the most common pseudomonal species.

Characteristic Members of the genus display the following defining characteristics; Rod shaped Gram-negative One or more polar flagella, providing motility Aerobic Non–spore forming positive catalase test positive oxidase test. Beta hemolytic (on blood agar) Indole negative Methyl red negative Citrate positive.

Pseudomonas colonies on agar plate

Pseudomonas with flagella

Diseases

Pseudomonal infection, as described by Pollack, occurs in 3 stages; (1) bacterial attachment and colonization, followed by (2) local invasion (3) dissemination and systemic disease

End…….. Q & A