Characterization of adenosine receptors in bovine chondrocytes and fibroblast-like synoviocytes exposed to low frequency low energy pulsed electromagnetic.

Slides:

Advertisements

Similar presentations

Trichostatin A, a histone deacetylase inhibitor, suppresses synovial inflammation and subsequent cartilage destruction in a collagen antibody-induced.

Advertisements

CXC chemokine ligand 12a enhances chondrocyte proliferation and maturation during endochondral bone formation G.-W. Kim, M.-S. Han, H.-R. Park, E.-J.

Effects of pleiotrophin, a heparin-binding growth factor, on human primary and immortalized chondrocytes Dr. T. Pufe, Ph.D., G. Groth, M.B. Goldring,

Induction of osteoclast-like cells derived from the synovial lavage fluids of patients with temporomandibular joint disorders H. Takano, D.D.S., W. Ariyoshi,

Moderate dynamic compression inhibits pro-catabolic response of cartilage to mechanical injury, tumor necrosis factor-α and interleukin-6, but accentuates.

W. L. Parker, M. D. , Ph. D. , K. W. Finnson, Ph. D. , H. Soe-Lin, B

L. J. Sandell, Ph. D. , X. Xing, M. D. , C. Franz, M. A. , S

Adenosine analogs and electromagnetic fields inhibit prostaglandin E2 release in bovine synovial fibroblasts M. De Mattei, Ph.D., K. Varani, Ph.D., F.F.

T. Minashima, K.A. Campbell, S.R. Hadley, Y. Zhang, T. Kirsch

Glutamate signaling in chondrocytes and the potential involvement of NMDA receptors in cell proliferation and inflammatory gene expression T. Piepoli,

MAPKs are essential upstream signaling pathways in proteolytic cartilage degradation – divergence in pathways leading to aggrecanase and MMP-mediated.

Adenosine-induced apoptosis in glomerular mesangial cells

Xibin Wang, Ph. D. , Paul A. Manner, M. D. , Alan Horner, Ph. D

Trichostatin A, a histone deacetylase inhibitor, suppresses synovial inflammation and subsequent cartilage destruction in a collagen antibody-induced.

Hyaluronan oligosaccharide treatment of chondrocytes stimulates expression of both HAS-2 and MMP-3, but by different signaling pathways I. Schmitz, W.

Volume 60, Issue 4, Pages (October 2001)

CXC chemokine ligand 12a enhances chondrocyte proliferation and maturation during endochondral bone formation G.-W. Kim, M.-S. Han, H.-R. Park, E.-J.

Endoglin differentially regulates TGF-β-induced Smad2/3 and Smad1/5 signalling and its expression correlates with extracellular matrix production and.

Role of hypoxia-inducible factor-1 alpha in the regulation of plasminogen activator activity in rat knee joint chondrocytes G. Zhu, Y. Tang, X. Liang,

Modulation of articular chondrocyte proliferation and anionic glycoconjugate synthesis by glucosamine (GlcN), N-acetyl GlcN (GlcNAc) GlcN sulfate salt.

NF-κBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1β-induced and TNF-α-induced chondrocytes Dr C. Lianxu, Ph.D.,

Histone deacetylase inhibitors suppress interleukin-1β-induced nitric oxide and prostaglandin E2 production in human chondrocytes N. Chabane, M.Sc.,

Restriction of spontaneous and prednisolone-induced leptin production to dedifferentiated state in human hip OA chondrocytes: role of Smad1 and β-catenin.

Effect of glucosamine and its peptidyl-derivative on the production of extracellular matrix components by human primary chondrocytes D. Stoppoloni, L.

John F. Öhd, Katarina Wikström, Anita Sjölander Gastroenterology

Chondrocyte calcium-sensing receptor expression is up-regulated in early guinea pig knee osteoarthritis and modulates PTHrP, MMP-13, and TIMP-3 expression

Expression and function of the insulin receptor in normal and osteoarthritic human chondrocytes: modulation of anabolic gene expression, glucose transport.

Differential expression of leptin and leptin's receptor isoform (Ob-Rb) mRNA between advanced and minimally affected osteoarthritic cartilage; effect.

P. -J. Francin, A. Abot, C. Guillaume, D. Moulin, A. Bianchi, P

Osteogenic Protein-1 inhibits matrix depletion in a hyaluronan hexasaccharide-induced model of osteoarthritis1 1 Supported in part by NIH grants P50-AR39239,

S. Varghese, Ph. D. , P. Theprungsirikul, B. S. , S. Sahani, B. S. , N

Volume 114, Issue 3, Pages (March 1998)

Glucosamine promotes chondrogenic phenotype in both chondrocytes and mesenchymal stem cells and inhibits MMP-13 expression and matrix degradation A.

Proteoglycan synthesis in bovine articular cartilage explants exposed to different low- frequency low-energy pulsed electromagnetic fields M. De Mattei,

Differentiation potential of human muscle-derived cells towards chondrogenic phenotype in alginate beads culture R. Andriamanalijaona, Ph.D., E. Duval,

T Pufe, M Bartscher, W Petersen, B Tillmann, R Mentlein

Expression and regulation of Toll-like receptor 2 by IL-1β and fibronectin fragments in human articular chondrocytes S.-L. Su, M.S., C.-D. Tsai, Ph.D.,

Inhibition of cyclooxygenase-2 expression and prostaglandin E2 production in chondrocytes by avocado soybean unsaponifiables and epigallocatechin gallate

Differential metabolic effects of glucosamine and N-acetylglucosamine in human articular chondrocytes A.R. Shikhman, D.C. Brinson, J. Valbracht, M.K.

Enrichment of committed human nucleus pulposus cells expressing chondroitin sulfate proteoglycans under alginate encapsulation Y. Sun, M. Lv, L. Zhou,

Evidence for two distinct pathways in TNFα-induced membrane and soluble forms of ICAM-1 in human osteoblast-like cells isolated from osteoarthritic patients

DIO2 modifies inflammatory responses in chondrocytes

Isolation (From a Basal Cell Carcinoma) of a Functionally Distinct Fibroblast-Like Cell Type that Overexpresses Ptch Anthony J. Dicker, Magdalena M.

Aurelia Lugea, Ilya Gukovsky, Anna S Gukovskaya, Stephen J Pandol

Expression of proteinase-activated receptors (PAR)-2 in articular chondrocytes is modulated by IL-1β, TNF-α and TGF-β Y. Xiang, M.D., K. Masuko-Hongo,

H Jo, M. D. , J. S Park, Ph. D. , E. M Kim, M. S. , M. Y Jung, Ph. D

Volume 74, Issue 12, Pages (December 2008)

Selenomethionine inhibits IL-1β inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX2) expression in primary human chondrocytes A.W.M. Cheng,

PCB126 induces apoptosis of chondrocytes via ROS-dependent pathways

Enhancing and maintaining chondrogenesis of synovial fibroblasts by cartilage extracellular matrix protein matrilins M. Pei, M.D., Ph.D., J. Luo, M.D.,

T. Kurth, M. Sc. , E. Hedbom, Ph. D. , N. Shintani, Ph. D. , M

Autologous chondrocyte implantation (ACI) for aged patients: development of the proper cell expansion conditions for possible therapeutic applications

Z. Lin, M. B. , N. J. Pavlos, B. Sc. (Hons. ), Ph. D. , M. A. Cake, B

Glutamate signaling in chondrocytes and the potential involvement of NMDA receptors in cell proliferation and inflammatory gene expression T. Piepoli,

S.D. Waldman, J. Usprech, L.E. Flynn, A.A. Khan

Glucosamine sulfate modulates the levels of aggrecan and matrix metalloproteinase-3 synthesized by cultured human osteoarthritis articular chondrocytes

Matrix metalloproteinase-13 influences ERK signalling in articular rabbit chondrocytes L.J. Raggatt, Ph.D., S.C. Jefcoat, M.S., I. Choudhury, Ph.D., S.

Activation of Indian hedgehog promotes chondrocyte hypertrophy and upregulation of MMP-13 in human osteoarthritic cartilage F. Wei, J. Zhou, X. Wei,

W. Wang, T. Hayami, S. Kapila Osteoarthritis and Cartilage

Mevastatin reduces cartilage degradation in rabbit experimental osteoarthritis through inhibition of synovial inflammation Y. Akasaki, M.D., S. Matsuda,

Membrane type-1 matrix metalloproteinase is induced following cyclic compression of in vitro grown bovine chondrocytes J.N.A. De Croos, Ph.D., B. Jang,

Spingosine-1-phosphate stimulates proliferation and counteracts interleukin-1 induced nitric oxide formation in articular chondrocytes M.H. Stradner,

Comparative effects of IL-1β and hydrogen peroxide (H2O2) on catabolic and anabolic gene expression in juvenile bovine chondrocytes G. Martin, Ph.D.,

Adenosine Receptors as Mediators of Both Cell Proliferation and Cell Death of Cultured Human Melanoma Cells Stefania Merighi, Prisco Mirandola, Daniela.

Chondroitin sulfate modulation of matrix and inflammatory gene expression in IL-1β- stimulated chondrocytes – study in hypoxic alginate bead cultures

Identification of opticin, a member of the small leucine-rich repeat proteoglycan family, in human articular tissues: a novel target for MMP-13 in osteoarthritis

Suppression of VEGFR2 Expression in Human Endothelial Cells by Dimethylfumarate Treatment: Evidence for Anti-Angiogenic Action Markus Meissner, Monika.

Peroxisome proliferator activated receptor alpha activation decreases inflammatory and destructive responses in osteoarthritic cartilage S. Clockaerts,

Volume 58, Issue 6, Pages (December 2000)

Effects of physical stimulation with electromagnetic field and insulin growth factor-I treatment on proteoglycan synthesis of bovine articular cartilage

Presentation transcript:

Characterization of adenosine receptors in bovine chondrocytes and fibroblast-like synoviocytes exposed to low frequency low energy pulsed electromagnetic fields K. Varani, Ph.D., M. De Mattei, Ph.D., F. Vincenzi, Sc.D., S. Gessi, Ph.D., S. Merighi, Ph.D., A. Pellati, Sc.D., A. Ongaro, Ph.D., A. Caruso, Ph.D., R. Cadossi, Ph.D., P.A. Borea, Ph.D. Osteoarthritis and Cartilage Volume 16, Issue 3, Pages 292-304 (March 2008) DOI: 10.1016/j.joca.2007.07.004 Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Immunohistochemistry for aggrecan (A), type II (B) and type I (C) collagens of bovine chondrocytes cultured in monolayers. Nuclei were counterstained by hematoxylin. Osteoarthritis and Cartilage 2008 16, 292-304DOI: (10.1016/j.joca.2007.07.004) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Expression of vimentin in synovial fibroblasts (A) (original magnification 20×). Analysis of CD14 mRNA expression (B upper panel) in macrophages (MC) and in bovine fibroblast-like synoviocytes (FS). M is 100bp DNA ladder (Biolabs). One microgram of total RNA was loaded for lane and stained with ethidium bromide to confirm equal RNA quantity used for RT-PCR (B lower panel). Osteoarthritis and Cartilage 2008 16, 292-304DOI: (10.1016/j.joca.2007.07.004) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Western blotting analysis of bovine chondrocytes and fibroblast-like synoviocytes in the absence and in the presence of PEMFs for A1, A2A and A2B adenosine receptors (A). Each band was quantified by using molecular analyst/PC densitometry software (Bio-Rad). Mean densitometry data from three independent experiments were normalized to control from chondrocytes (B) and fibroblast-like synoviocytes (C). Osteoarthritis and Cartilage 2008 16, 292-304DOI: (10.1016/j.joca.2007.07.004) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Saturation of [3H]-ZM 241385 binding to A2A adenosine receptors (A) and of [3H]-MRE 3008F20 binding to A3 adenosine receptors (C) on untreated or PEMF-treated bovine chondrocyte membranes. Experiments were performed as described in Materials and methods. Values are the means and vertical lines s.e.m. of six separate experiments performed in triplicate. The Scatchard plots of the same data are shown in panels B and D. Osteoarthritis and Cartilage 2008 16, 292-304DOI: (10.1016/j.joca.2007.07.004) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Saturation of [3H]-ZM 241385 binding to A2A adenosine receptors (A,B) and of [3H]-MRE 3008F20 binding to A3 adenosine receptors (C,D) on untreated or PEMF-treated bovine fibroblast-like synoviocyte membranes. Experiments were performed as described in Materials and methods. Values are the means and vertical lines s.e.m. of six separate experiments performed in triplicate. The Scatchard plots of the same data are shown in panels B and D. Osteoarthritis and Cartilage 2008 16, 292-304DOI: (10.1016/j.joca.2007.07.004) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 6 Van't Hoff plot showing the effect of temperature on the equilibrium binding association constant, KA=1/KD of [3H]-ZM 241385 to A2A adenosine receptors (A,C) or [3H]-MRE 3008F20 to A3 adenosine receptors (B,D) in untreated or PEMF-treated bovine fibroblast-like synoviocyte membranes, respectively. The plots are essentially linear in the temperature range investigated (4–30°C). Binding experiments were performed as described in Materials and methods (n=3). Osteoarthritis and Cartilage 2008 16, 292-304DOI: (10.1016/j.joca.2007.07.004) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 7 Competition experiments of specific [3H]-ZM 241385 to A2A adenosine receptors (A,C) or [3H]-MRE 3008F20 binding to A3 adenosine receptors (B,D) of CGS 21680 or Cl-IB-MECA in untreated and PEMF-treated bovine chondrocytes (A,B) or fibroblast-like synoviocyte (C,D) membranes. Competition experiments were performed as described in Materials and methods (n=4). Osteoarthritis and Cartilage 2008 16, 292-304DOI: (10.1016/j.joca.2007.07.004) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 8 cAMP experiments by using a typical A2A agonist, CGS 21680 (A,C) or a typical A3 agonist, Cl-IB-MECA (B,D) in untreated and PEMF-treated bovine chondrocytes (A,B) or fibroblast-like synoviocytes (C,D). cAMP experiments were performed as described in Materials and methods (n=4). Osteoarthritis and Cartilage 2008 16, 292-304DOI: (10.1016/j.joca.2007.07.004) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 9 Proliferation activity measured by [3H] thymidine incorporation assay. Bovine chondrocytes (A) or fibroblast-like synoviocytes (B) were treated with CGS 21680 and Cl-IB-MECA at 10μM concentration. Osteoarthritis and Cartilage 2008 16, 292-304DOI: (10.1016/j.joca.2007.07.004) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions