Oral contraceptives suppress cell proliferation and enhance apoptosis of eutopic endometrial tissue from patients with endometriosis Gabriela F. Meresman,

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Oral contraceptives suppress cell proliferation and enhance apoptosis of eutopic endometrial tissue from patients with endometriosis Gabriela F. Meresman, Ph.D., Luis Augé, M.D., Rosa I. Barañao, Ph.D., Eduardo Lombardi, M.D., Marta Tesone, Ph.D., Carlos Sueldo, M.D. Fertility and Sterility Volume 77, Issue 6, Pages 1141-1147 (June 2002) DOI: 10.1016/S0015-0282(02)03099-6

FIGURE 1 Cell proliferation at the EP (A) and ST levels (B) in eutopic endometria from patients with EDT before (EDT-PRE) and after (EDT-POST) COC administration and in control women (CONTROL). Histological sections were immunostained for Ki-67 expression (see text). The Ki-67 index was determined by counting labeled cells in 400× randomly selected fields. Data were expressed as the number of EP cells expressing immunoreactivity for Ki-67 per 100 cells (Ki-67 index) (A) and as the number of cells expressing immunoreactivity for Ki-67 (positive) ST cells per field (B). ∗P<.001 vs. EDT-PRE; ∗∗P<.05 vs. EDT-PRE; EDT-POST vs. CONTROL, P>.05. Meresman. Oral contraceptives and endometriosis. Fertil Steril 2002. Fertility and Sterility 2002 77, 1141-1147DOI: (10.1016/S0015-0282(02)03099-6)

FIGURE 3 Representative photomicrograph of histological sections from the eutopic endometrium of women with EDT. Immunostaining of endometrial sections for cell proliferation by Ki-67 expression before administering COCs (A) and for apoptosis by the TUNEL technique after COC administration (B) is shown. Note the presence of numerous immunostained cells. The original magnification was ×400. Meresman. Oral contraceptives and endometriosis. Fertil Steril 2002. Fertility and Sterility 2002 77, 1141-1147DOI: (10.1016/S0015-0282(02)03099-6)

FIGURE 2 Spontaneous apoptosis at the EP (A) and ST levels (B) in eutopic endometria from patients with EDT before (EDT-PRE) and after (EDT-POST) COC administration and in control women (CONTROL). Histological sections were immunostained by the TUNEL technique (see text). The numbers of apoptotic cells were determined by counting labeled cells in 630× randomly selected fields. Data were expressed as the apoptotic cell mean/field. ∗P<.001 vs. EDT-PRE; ∗∗P<.05 vs. EDT-PRE; EDT-POST vs. CONTROL, P>.05. Meresman. Oral contraceptives and endometriosis. Fertil Steril 2002. Fertility and Sterility 2002 77, 1141-1147DOI: (10.1016/S0015-0282(02)03099-6)

FIGURE 4 A and B are sections immunostained for Bcl-2 and Bax protein expression. Bcl-2 protein expression in proliferative endometrium from a patient with EDT before administering COCs, magnification ×400 (A). Bax protein expression in the endometrium from the same patient after administration of COCs, magnification ×400. Note the strong immunopositivity in endometrial sections. Meresman. Oral contraceptives and endometriosis. Fertil Steril 2002. Fertility and Sterility 2002 77, 1141-1147DOI: (10.1016/S0015-0282(02)03099-6)