Dose response of pAGA1 and pFIG1 induction

Slides:

Advertisements

Similar presentations

Fus3 is required for deletions of GIM4 and BIM1 to increase cell‐to‐cell variability Fus3 is required for deletions of GIM4 and BIM1 to increase cell‐to‐cell.

Advertisements

Cluster analysis of 50 genes identified as affecting variability and or pheromone response output Cluster analysis of 50 genes identified as affecting.

Microtubule perturbations cause Ste5 patches to form less reliably, delay patch formation, and cause patches to persist for less time Microtubule perturbations.

Microtubule perturbations affect pathway variability η2(P) and transmitted signal P at or upstream of the Ste5 recruitment step Microtubule perturbations.

Juxtavascular astrocytes are enriched in nuclear Aryl hydrocarbon receptor immunostaining at the stab wound injury site in WT mice Juxtavascular astrocytes.

PLAUhigh Tregs exhibit higher potential in suppressing effector T cells and express higher levels of Treg‐important genes, but they express lower levels.

Periodic changes in distribution of ERK–GFP fusion protein in cells after stimulation with EGF. (A) Confocal image of cells expressing ERK–GFP both before.

Statistical analysis of the influence of network regulators on topology clusters in the oleate network. Statistical analysis of the influence of network.

Network topology reflects target gene expression profile and function.

A kinetic model reconciles observed ERK phosphorylation, localization, and activity responses A Schematic of a simple kinetic model including cytosolic.

Analysis of mutational effects of parameters on the phenotype (i. e

Most promoters preserve their relative activity levels across conditions. Most promoters preserve their relative activity levels across conditions. (A)

Overexpression of RNase H1 decreases the induction of phosphorylated H2AX in post‐mitotic cells in response to topoisomerase 1 cleavage complexes. Overexpression.

Dual allele labeling reveals a trans‐acting source for extrinsic noise

Tumorigenic attributes of FUT9

The TBON model. The TBON model. (A) Representative confocal images of E14Tg2A cells stained for Tcf3 (green), Nanog (red), Oct4 (magenta), and total β‐catenin.

The human STRIPAK complex associates with RASF3 and MST1/2

Expression of FUT9 supports tumor development

Characterization of promoters relative to pAGA1

Dynamics and variability of SMAD2 signaling in single cells

Evaluating CRISPR negative selection screens.

Genomic profiling of fitness in periodic salt stress

Detection of specific interacting partners of CBL and CBLB in peripheral CD4+ T cells prior to and following TCR stimulation Detection of specific interacting.

The PRS is robust to changes in receptor abundance

A coherent feed‐forward motif sharpens the Dnmt3b transcriptional output into a bistable switch. A coherent feed‐forward motif sharpens the Dnmt3b transcriptional.

Dermal architecture is defined by an inverse correlation between fibroblast proliferation and ECM deposition Dermal architecture is defined by an inverse.

RNAi causes widespread changes in cell population context leading to predictable changes in virus infection. RNAi causes widespread changes in cell population.

Mutants of the Group III have differentially impaired induction of pAGA1 and pFIG1 Mutants of the Group III have differentially impaired induction of pAGA1.

Validation of some ePTL candidates.

Dynamic map of the Nap1p/Kcc4p interaction.

Changes to the growth conditions break the circuit by changing host gene expression Changes to the growth conditions break the circuit by changing host.

Nec‐1 inhibits the phosphorylation and aggregation of tau

Lysozyme expression in isolated Paneth cells and linage tracing of Notch1+ cells Lysozyme expression in isolated Paneth cells and linage tracing of Notch1+

Collective shifts of abundance for nuclear and extracellular proteins in colorectal cancer Collective shifts of abundance for nuclear and extracellular.

Fibroblast proliferation and ECM changes during wound healing

Effect of the loss of Kar4 on the induction of various promoters

Dynamics and expression level of mating‐dependent promoters

Computational wound healing gradients and in vivo imaging of the wound bed at PW2 (related to Fig 6)‏ Computational wound healing gradients and in vivo.

Validation of knock‐in cell line and image calibration (related to Fig 1)‏ Validation of knock‐in cell line and image calibration (related to Fig 1) Validation.

Comparison of proteomics and RNA‐Seq data.

Comparison of nuclear surface area of HeLa and RKO cells

Dynamics of induction of mating promoters after pheromone stimulation

Effects of ERK–GFP expression levels and cell density on ERK phosphorylation and oscillations. Effects of ERK–GFP expression levels and cell density on.

Development of a 3D tissue model and in vivo live imaging during dermal maturation Development of a 3D tissue model and in vivo live imaging during dermal.

Determination of mRNA synthesis and decay rates.

Simulation showing that the cell length variability of the entire population can mask abnormal cell length variability at a specific cell cycle period.

Transformation of expression data to identify more direct consequences of perturbation Expression changes under amino acid starvation conditions (30 min,

An integrated NHR network.

Design and optimization of the computational model.

Differential stress sensitivity of the dividing versus non‐dividing cells. Differential stress sensitivity of the dividing versus non‐dividing cells. (A)

Distinct collagen structures in the upper and lower neonatal dermis (related to Fig 1)‏ Distinct collagen structures in the upper and lower neonatal dermis.

Effect of HDAC inhibition on the steady‐state and dynamic transcriptional response and associated noise. Related to Fig 7 Effect of HDAC inhibition on.

Antisense‐mediated regulation of SUR7.

Noise in hoxb1a/krox20 expression leads to boundary sharpening.

Antisense expression associates with larger gene expression variability. Antisense expression associates with larger gene expression variability. (A–D)

Experimental validation of predicted endocytosis functions in human.

Patterns and regulation of age‐related splicing changes.

Time‐course analysis of NICD degradation.

Single‐cell RT–qPCR data

Characterization results of the lysis device in the final system using 3OC12HSL. Characterization results of the lysis device in the final system using.

The transcriptional behavior of GREB1 changes with estrogen dose and exhibits considerable cell‐to‐cell variation (see also Fig EV2 and Movie EV2)‏ The.

Perturbation of ligand depletion affects the ultrasensitivity of long‐term P‐Smad2 responses to different doses of TGF‐β stimulation. Perturbation of ligand.

(A) Observed significant protein fold‐changes during the fed–fasting transition in C57BL/6J (B6) and 129Sv (S9) mice fed with a normal diet (T0). (A) Observed.

Inhibitory effect of perhexiline on the proliferation of the HepG2 cell line A, BPerhexiline was used to mimic the effect of the l‐carnitine analog on.

Kinetics of GREB1 induction by estrogen in single cells (see also Fig EV4 and Movie EV3)‏ Kinetics of GREB1 induction by estrogen in single cells (see.

Growth factor‐stimulated ERK kinase activities in the cytosol and nucleus are kinetically ordered and do not show dramatic adaptation Growth factor‐stimulated.

CRISPR‐Cas9 gene perturbation profiling in HeLa cells

Chd5 deficiency leads to compromised expression of the repressive histone mark H3K27me3 and up-regulation of ribosomal protein genes. Chd5 deficiency leads.

Adipocytes sequester daunorubicin (DNR).

Plasma concentration of metabolite M1 (left panel), glucose infusion rate (GIR) to maintain euglycemia (middle panel), and blood glucose concentration.

Presentation transcript:

Dose response of pAGA1 and pFIG1 induction Dose response of pAGA1 and pFIG1 induction A, BMean expression output for pAGA1 (A) and pFIG1 (B) in response to different pheromone concentrations, in a WT or bar1∆ (shaded) background. The expression output is defined as the maximal dPSTR nuclear enrichment following stimulation, for all cells of the experiment. Error bars represent the standard deviation of three replicates. Note that the induction of pAGA1 gradually increases with the pheromone concentration, whereas pFIG1 displays a switch‐like response in WT. Note that in a bar1∆ background, the expression occurs at lower concentrations and with higher level for both promoters. The first dot is the non‐induced control.CPercentage of cells expressing pAGA1 (red) or pFIG1 (blue) in a WT (upper panel) or bar1∆ (lower panel) background, at various pheromone concentrations for one representative experiment. Note that only at high concentrations, a significant proportion of the population expresses pFIG1.DMedian nuclear enrichment of the pAGA1‐dPSTRY (red, left axis) and of the pFIG1‐dPSTRR (blue, right axis) in course of time, for the different pheromone concentration, in the WT background, for one representative experiment. The solid line is the median, and the shaded area represents the 25th–75th percentile. The reference curves at 1 μM are represented in dashed line for comparison. Note that the scale of the pFIG1‐dPSTRR is different between the 1 μM and the other concentrations. Delphine Aymoz et al. Mol Syst Biol 2018;14:e8024 © as stated in the article, figure or figure legend