Wide geographic distribution of a unique methicillin-resistant Staphylococcus aureus clone in Hungarian hospitals Herminia de Lencastre, Elena P. Severina,

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Presentation transcript:

Wide geographic distribution of a unique methicillin-resistant Staphylococcus aureus clone in Hungarian hospitals Herminia de Lencastre, Elena P. Severina, Hedda Milch, Marianne Konkoly Thege, Alexander Tomasz Clinical Microbiology and Infection Volume 3, Issue 3, Pages 289-296 (June 1997) DOI: 10.1111/j.1469-0691.1997.tb00616.x Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1 Macrorestriction patterns of chromosomal SmaI digests, prepared from the Hungarian MRSA isolates. DNA preparation, restriction and separation of DNA fragments by PFGE were as described in the text. Leftmost and rightmost lanes and a lane in the middle of the gel have molecular weight markers (Λ ladder), corresponding to the molecular sizes (kb) of 646, 381, 186, 119, 93 and 48. In (d), lanes to the left of the lane with low-molecular-weight Λ markers (Λ l.m.w.) contain digests prepared from two Portuguese isolates (CP22 and 25, De Lencastre et al [4]) and from 10 Brazilian MRSA isolates [9], which were included for comparison. Clinical Microbiology and Infection 1997 3, 289-296DOI: (10.1111/j.1469-0691.1997.tb00616.x) Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 2 Southern hybridization of Hungarian MRSA isolates with a variety of DNA probes. (a) Chromosomal SmaI digests were hybridized with the mecA-specific DNA probe, in order to determine the molecular size of mecA-hybridizing DNA fragments. (b,c) Southern hybridization of chromosomal ClaI digests with a mecA-specific DNA probe (b) and with a Tn554 DNA probe (c). Chromosomal DNA preparation and Southern hybridization were performed as described in the text. Lanes are marked for strain identification. Molecular weight markers (HindIII digest of Λ) and the laboratory strain COL (carrying mecA polymorph II) are also included. Clinical Microbiology and Infection 1997 3, 289-296DOI: (10.1111/j.1469-0691.1997.tb00616.x) Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 3 Geographic distance separating the MRSA isolation sites. The map shows the locations and names of the six provincial towns in Hungary from whose hospitals the MRSA isolates analyzed were obtained. Clinical Microbiology and Infection 1997 3, 289-296DOI: (10.1111/j.1469-0691.1997.tb00616.x) Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions