Fluorescence in Situ Hybridization Analysis of Immunoglobulin Heavy Chain Translocations in Plasma Cell Myeloma Using Intact Paraffin Sections and Simultaneous.

Slides:

Advertisements

Similar presentations

Development of concurrent plasma cell FISH and immuno-fluorescent staining for use in the diagnosis of multiple myeloma. Dave Wallace - Cytogenetics.

Advertisements

Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,

CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.

Association of Clinical Status of Follicular Lymphoma Patients after Autologous Stem Cell Transplant and Quantitative Assessment of Lymphoma in Blood and.

Kazunori Kanehira, Douglas L. Riegert-Johnson, Dong Chen, Lawrence E

Intratumoral Patterns of Clonal Evolution in Meningiomas as Defined by Multicolor Interphase Fluorescence in Situ Hybridization (FISH) José María Sayagués,

Automated Quantitative RNA in Situ Hybridization for Resolution of Equivocal and Heterogeneous ERBB2 (HER2) Status in Invasive Breast Carcinoma Zhen.

Detection of TMPRSS2-ETS Fusions by a Multiprobe Fluorescence in Situ Hybridization Assay for the Early Diagnosis of Prostate Cancer Qi-Peng Sun, Liao-Yuan.

Droplet Digital PCR for Absolute Quantification of EML4-ALK Gene Rearrangement in Lung Adenocarcinoma Qiushi Wang, Xin Yang, Yong He, Qiang Ma, Li Lin,

Volume 52, Issue 1, Pages (July 2007)

Tracy I. George, Joanna E. Wrede, Charles D. Bangs, Athena M

Chromogenic In Situ Hybridization for α6β4 Integrin in Breast Cancer

The Frequency of Immunoglobulin Heavy Chain Gene and T-Cell Receptor γ-Chain Gene Rearrangements and Epstein-Barr Virus in ALK+ and ALK− Anaplastic Large.

Detection of TMPRSS2-ETS Fusions by a Multiprobe Fluorescence in Situ Hybridization Assay for the Early Diagnosis of Prostate Cancer Qi-Peng Sun, Liao-Yuan.

Cyclin D1-negative mantle cell lymphoma with cryptic t(12;14)(p13;q32) and cyclin D2 overexpression by Christian Herens, Frédéric Lambert, Leticia Quintanilla-Martinez,

by Rafael Fonseca, Richard J. Bailey, Gregory J. Ahmann, S

Quantitative Fluorescence in Situ Hybridization and its Ability to Predict Bladder Cancer Recurrence and Progression to Muscle-Invasive Bladder Cancer

Tony L. Ng, Maureen J. O'Sullivan, Catherine J

Harvey A. Greisman, Noah G. Hoffman, Hye Son Yi

Automated Quantitative RNA in Situ Hybridization for Resolution of Equivocal and Heterogeneous ERBB2 (HER2) Status in Invasive Breast Carcinoma Zhen.

FISH Analysis for the Detection of Lymphoma-Associated Chromosomal Abnormalities in Routine Paraffin-Embedded Tissue Roland A. Ventura, Jose I. Martin-Subero,

Microfluidic Chips for Detecting the t(4;14) Translocation and Monitoring Disease during Treatment Using Reverse Transcriptase-Polymerase Chain Reaction.

Jennelle C. Hodge, Patrick P. Bedroske, Kathryn E. Pearce, William R

Sarah Chiang, Ladan Fazlollahi, Anhthu Nguyen, Rebecca A

Kazunori Kanehira, Douglas L. Riegert-Johnson, Dong Chen, Lawrence E

Accurate Molecular Characterization of Formalin-Fixed, Paraffin-Embedded Tissues by microRNA Expression Profiling Anna E. Szafranska, Timothy S. Davison,

Detection of Genetic Alterations by ImmunoFISH Analysis of Whole Cells Extracted from Routine Biopsy Material Göran Mattsson, Soo Yong Tan, David J.P.

Analysis of HER2 Gene Amplification Using an Automated Fluorescence in Situ Hybridization Signal Enumeration System Rachel Stevens, Imad Almanaseer,

Molecular Diagnosis in Ewing Family Tumors

Fluorescence In Situ Hybridization

Undifferentiated Small Round Cell Sarcomas with Rare EWS Gene Fusions

Molecular Analysis of Gene Fusions in Bone and Soft Tissue Tumors by Anchored Multiplex PCR–Based Targeted Next-Generation Sequencing Suk Wai Lam, Anne-Marie.

The Development of a Multitarget, Multicolor Fluorescence in Situ Hybridization Assay for the Detection of Urothelial Carcinoma in Urine Irina A. Sokolova,

William L. Gerald, M.D., Ph.D, 1954–2008

Brenton T. Tan, Roger A. Warnke, Daniel A. Arber

Multiplex Ligation-Dependent Probe Amplification Versus Multiprobe Fluorescence in Situ Hybridization To Detect Genomic Aberrations in Chronic Lymphocytic.

Intratumoral Patterns of Clonal Evolution in Meningiomas as Defined by Multicolor Interphase Fluorescence in Situ Hybridization (FISH) José María Sayagués,

Development of an NPM1/MLF1 D-FISH Probe Set for the Detection of t(3;5)(q25;q35) Identified in Patients with Acute Myeloid Leukemia Umut Aypar, Ryan.

Chromosomal Biomarkers for Detection of Human Papillomavirus Associated Genomic Instability in Epithelial Cells of Cervical Cytology Specimens Irina.

Analytical Detection of Immunoglobulin Heavy Chain Gene Rearrangements in Gastric Lymphoid Infiltrates by Peak Area Analysis of the Melting Curve in the.

Capillary Electrophoresis Artifact Due to Eosin

Raymond R. Tubbs, James Pettay, Pat Roche, Mark H

Diagnosis of BK Viral Nephropathy in the Renal Allograft Biopsy

Dual IHC and FISH Testing for ALK Gene Rearrangement in Lung Adenocarcinomas in a Routine Practice: A French Study Anne McLeer-Florin, PhD, Denis Moro-Sibilot,

The MECT1-MAML2 Gene Fusion and Benign Warthin's Tumor

A Practical Approach to the Detection of Prognostically Significant Genomic Aberrations in Multiple Myeloma Zhong Chen, Bonnie Issa, Shiang Huang, Emily.

Molecular Pathology of Bone Tumors

Chromosomal Abnormalities in Non-Small Cell Lung Carcinomas and in Bronchial Epithelia of High-Risk Smokers Detected by Multi-Target Interphase Fluorescence.

Daynna J. Wolff, Adam Bagg, Linda D. Cooley, Gordon W. Dewald, Betsy A

Defining Ploidy-Specific Thresholds in Array Comparative Genomic Hybridization to Improve the Sensitivity of Detection of Single Copy Alterations in Cell.

Molecular Cytogenetic Analyses of Immunoglobulin Loci in Nodular Lymphocyte Predominant Hodgkin's Lymphoma Reveal a Recurrent IGH-BCL6 Juxtaposition

Successful Application of a Direct Detection Slide-Based Sequential Phenotype/Genotype Assay Using Archived Bone Marrow Smears and Paraffin Embedded.

Pulmonary Cytolytic Thrombi after Allogeneic Hematopoietic Cell Transplantation: A Further Histologic Description Antoinette Peters, J. Carlos Manivel,

Array Comparative Genomic Hybridization Detects Chromosomal Abnormalities in Hematological Cancers That Are Not Detected by Conventional Cytogenetics

Maria Erali, David C. Pattison, Carl T. Wittwer, Cathy A. Petti

Automated Multiplexing Quantum Dots in Situ Hybridization Assay for Simultaneous Detection of ERG and PTEN Gene Status in Prostate Cancer Wenjun Zhang,

The Detection of t(14;18) in Archival Lymph Nodes

Long Polymerase Chain Reaction-Based Fluorescence in Situ Hybridization Analysis of Female Carriers of X-Linked Chronic Granulomatous Disease Deletions

″Minor″ BCL2 Breakpoints in Follicular Lymphoma

Development of Five Dual-Color, Double-Fusion Fluorescence in Situ Hybridization Assays for the Detection of Common MLL Translocation Partners Jeannette.

Diagnosis of BK Viral Nephropathy in the Renal Allograft Biopsy

Statistical Treatment of Fluorescence in Situ Hybridization Validation Data to Generate Normal Reference Ranges Using Excel Functions Allison L. Ciolino,

Immunohistochemistry is a Reliable Screening Tool for Identification of ALK Rearrangement in Non–Small-Cell Lung Carcinoma and is Antibody Dependent

Karen Snow-Bailey, Ph.D., 1961–2006

Telomeric IGH Losses Detectable by Fluorescence in Situ Hybridization in Chronic Lymphocytic Leukemia Reflect Somatic VH Recombination Events Iwona Wlodarska,

Jie Hu, Malini Sathanoori, Sally J. Kochmar, Urvashi Surti

Fluorescence In Situ Hybridization Identifies Cryptic t(16;16)(p13;q22) Masked By del(16)(q22) in a Case of AML-M4 Eo Shakil H. Merchant, Skip Haines,

Monique A. Johnson, Marvin J. Yoshitomi, C. Sue Richards

Primary Cutaneous CD30-Positive T-Cell Lymphoproliferative Disorders with Biallelic Rearrangements of DUSP22 Courtney R. Csikesz, Ryan A. Knudson, Patricia.

Clinical Lymphoma, Myeloma and Leukemia

Presentation transcript:

Fluorescence in Situ Hybridization Analysis of Immunoglobulin Heavy Chain Translocations in Plasma Cell Myeloma Using Intact Paraffin Sections and Simultaneous CD138 Immunofluorescence James R. Cook, Marybeth Hartke, James Pettay, Raymond R. Tubbs The Journal of Molecular Diagnostics Volume 8, Issue 4, Pages 459-465 (September 2006) DOI: 10.2353/jmoldx.2006.050149 Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 CD138/FISH analysis of IGH translocations in CD138+ cell lines. The specificity of the FISH probes in intact paraffin sections was confirmed using the t(11;14)(q13;q32)-positive JVM2 cell line and the t(4;14)(p13;q32)-positive OPM2 cell line. Dashed lines indicate cutoff thresholds for interpretation as a positive result. The Journal of Molecular Diagnostics 2006 8, 459-465DOI: (10.2353/jmoldx.2006.050149) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Paraffin section FISH analysis with CD138 immunofluorescence. Under a 4′-6-Diamidino-2-phenylindole filter, CD138+ plasma cells are identified by their intense blue cytoplasmic staining. Other bone marrow elements remain unlabeled. The Journal of Molecular Diagnostics 2006 8, 459-465DOI: (10.2353/jmoldx.2006.050149) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 FISH analysis of IGH translocations in plasma cell myeloma. A: Analysis with a break-apart probe spanning the IGH locus identifies one pair of juxtaposed red (centromeric) and green (telomeric) signals, and one pair of split red and green signals consistent with an IGH translocation. B: Further analysis with a dual fusion probe for t(11;14)(q13;q32) identifies two separate red (CCND1) and green (IGH) signals, indicating absence of the IGH/CCND1 translocation. C: A dual fusion probe for t(4;14)(p13;q32) shows separate red (MMSET) and green (IGH) signals, and two juxtaposed (fusion) signals, identifying this IGH translocation as t(4;14)(p13;q32). The Journal of Molecular Diagnostics 2006 8, 459-465DOI: (10.2353/jmoldx.2006.050149) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 4 Percentage of abnormal CD138+ nuclei in translocation-positive myeloma. When abnormalities were present, they were seen in a large percentage of CD138+ nuclei scored (median = 84%). The percentage of abnormal CD138+ nuclei seen was similar across the range of bone marrow plasma cell counts tested. The Journal of Molecular Diagnostics 2006 8, 459-465DOI: (10.2353/jmoldx.2006.050149) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions