Proteomic identification of differentially expressed proteins in aortic wall of patients with ruptured and nonruptured abdominal aortic aneurysms Sigitas.

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Proteomic identification of differentially expressed proteins in aortic wall of patients with ruptured and nonruptured abdominal aortic aneurysms Sigitas Urbonavicius, MD, PhD, Jes S. Lindholt, MD, PhD, Henrik Vorum, MD, PhD, Grazina Urbonaviciene, MD, Eskild W. Henneberg, MD, Bent Honoré, MD, Dr Med Sci Journal of Vascular Surgery Volume 49, Issue 2, Pages 455-463 (February 2009) DOI: 10.1016/j.jvs.2008.08.097 Copyright © 2009 The Society for Vascular Surgery Terms and Conditions

Fig 1 Two-dimensional gel images of non-ruptured abdominal aortic aneurysms (A) and ruptured abdominal aortic aneurysms (B). Proteins that were significantly differentially expressed more than two-fold are indicated with arrows (upward pointing, up-regulated; downward pointing, down-regulated). Five of the proteins were identified using LC-MS/MS as indicated in Table I where also the degree of up- and down-regulation is given. Journal of Vascular Surgery 2009 49, 455-463DOI: (10.1016/j.jvs.2008.08.097) Copyright © 2009 The Society for Vascular Surgery Terms and Conditions

Fig 2 Protein levels as spot volume percentages of seven significantly and more than two-fold differentially expressed proteins from non-ruptured (∘) and ruptured (•) abdominal aneurysms. The mean values in each group are indicated and connected. Journal of Vascular Surgery 2009 49, 455-463DOI: (10.1016/j.jvs.2008.08.097) Copyright © 2009 The Society for Vascular Surgery Terms and Conditions

Fig 3 Identification of spot No. 605 by LC-MS/MS. Three tandem mass spectra used to reliably identify spot 605 as peroxiredoxin-2. The identified peptides are indicated with bold in the sequence. Journal of Vascular Surgery 2009 49, 455-463DOI: (10.1016/j.jvs.2008.08.097) Copyright © 2009 The Society for Vascular Surgery Terms and Conditions

Fig 4 Identification of spot No. 130 by LC-MS/MS. From spot No. 130 two tandem mass spectra were obtained. A, Shows a high quality spectrum that is compatible with a peptide found in vitronectin (shown with bold). B, A different peptide from spot No. 130 showed a borderline significance with a peptide in calreticulin (shown with bold). Journal of Vascular Surgery 2009 49, 455-463DOI: (10.1016/j.jvs.2008.08.097) Copyright © 2009 The Society for Vascular Surgery Terms and Conditions

Fig 5 Western blotting analysis using anti-peroxiredoxin-2 and anti-vitronectin on 5 patient samples (1-5) from non-ruptured AAA group (A) and 6 patient samples (1-6) from ruptured AAA group (B). The bands indicated with arrows are significantly differentially regulated in the ruptured group using t test (P < .05). Peroxiredoxin-2 is up-regulated and two vitronectin bands quantitated together (indicated with arrows) are down-regulated in the ruptured group (C). Journal of Vascular Surgery 2009 49, 455-463DOI: (10.1016/j.jvs.2008.08.097) Copyright © 2009 The Society for Vascular Surgery Terms and Conditions