Mass spectrometry Detection of ionic species to determine the molecular weight of and obtain structural information on a molecule.

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Presentation transcript:

Mass spectrometry Detection of ionic species to determine the molecular weight of and obtain structural information on a molecule

Mass spectrometry

ions only detected individual molecules are detected uses isotopic masses not MW

29 Base peak Parent ion Base Peak Parent ion -15 -CH3

43 58 29 44 Acetone Acetaldehyde -15 -CH3 -15 -CH3

Isotope effects Molecular weight Br = 79.9040 Br isotopic abundance CH379Br CH381Br

Isotope effects Molecular weight Br = 79.9040 Br isotopic abundance CH379Br CH381Br

Isotope effects Molecular weight Cl = 35.453 Cl isotopic abundance 35Cl : 37Cl Ratio = 3:1 CH335Cl CH337Cl

MS Ionization Techniques High energy hard ionization Low energy soft ionization Electron Impact (EI) uses beam of electrons Fast Atom Bombardment (FAB) uses beam of atoms Chemical Ionization (CI) uses chemical reactions Matrix Assisted Laser Desorption (MALDI) uses lasers to ionize molecules Electrospray uses compounds which are already ionic