Clonal dissemination of a toxin-A-negative/toxin-B-positive Clostridium difficile strain from patients with antibiotic-associated diarrhea in Poland

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Clonal dissemination of a toxin-A-negative/toxin-B-positive Clostridium difficile strain from patients with antibiotic-associated diarrhea in Poland H. Pituch, N. van den Braak, W. van Leeuwen, A. van Belkum, G. Martirosian, P. Obuch-Woszczatyński, M. Łuczak, F. Meisel- Mikołajczyk Clinical Microbiology and Infection Volume 7, Issue 8, Pages 442-446 (August 2001) DOI: 10.1046/j.1198-743x.2001.00312.x Copyright © 2001 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1 Genotypic characterisation of C. difficile strains. (A) Results of PCR ribotyping for four different strains. Strain numbers are indicated above the lanes and the corresponding types are A, H, I and A from left to right. (B) Results of an RAPD test employing primer AP7. Note that the banding patterns for strains 1, 3, 4, 5, 6 and 9, representing the prevalent A-type, are indiscriminate. On the left of both panels, the 600-bp DNA size marker band is indicated (M: 100-bp size ladder (Bio-Rad, Veenendaal, The Netherlands)). Clinical Microbiology and Infection 2001 7, 442-446DOI: (10.1046/j.1198-743x.2001.00312.x) Copyright © 2001 European Society of Clinical Infectious Diseases Terms and Conditions