Ex vivo expansion of megakaryocyte precursors from umbilical cord blood CD34+ cells in a closed liquid culture system Peter H. Shaw, Devin Gilligan,

Slides:

Advertisements

Similar presentations

Box and Whisker Plots Example: Comparing two samples.

Advertisements

A Simplified Procedure for Hematopoietic Stem Cell Amplification Using a Serum-Free, Feeder Cell-Free Culture System Ian M. Rogers, Nobuko Yamanaka, Robert.

Characterization of Cord Blood Natural Killer and Lymphokine Activated Killer Lymphocytes Following Ex Vivo Cellular Engineering Janet Ayello, Carmella.

Serial Profile of Vitamins and Trace Elements during the Acute Phase of Allogeneic Stem Cell Transplantation Yasuhito Nannya, Akihito Shinohara, Motoshi.

Reduced Frequency of Regulatory T Cells in Peripheral Blood Stem Cell Compared to Bone Marrow Transplantations Céline Blache, Joe-Marc Chauvin, Aude.

How to Treat MDS without Stem Cell Transplantation

Suzanne L. Tomchuck, Wing H. Leung, Mari H. Dallas

Vaccination of Children following Allogeneic Stem Cell Transplantation

Recovery and Safety Profiles of Marrow and PBSC Donors: Experience of the National Marrow Donor Program John P. Miller, Elizabeth H. Perry, Thomas H.

Peter Hurley, Suma Konety, Qing Cao, Daniel Weisdorf, Anne Blaes

Association of an Impaired Bone Marrow Microenvironment with Secondary Poor Graft Function after Allogeneic Hematopoietic Stem Cell Transplantation Yuan.

Infusion of Ex Vivo Expanded Cord Blood Progenitor Cells Is Associated with Reduced Hospital Days and Utilization of Opiate Infusion and Total Parenteral.

Adoptive Cellular Therapy using Cells Enriched for NKG2D+CD3+CD8+T Cells after Autologous Transplantation for Myeloma Kenneth R. Meehan, Laleh Talebian,

Allogeneic Stem Cell Transplantation in Myelofibrosis

Cost and Clinical Analysis of Autologous Hematopoietic Stem Cell Mobilization with G- CSF and Plerixafor Compared to G-CSF and Cyclophosphamide Paul Shaughnessy,

Bradley W. Blaser, Haesook T. Kim, Edwin P. Alyea, Vincent T

Definition of the Variables Affecting Efficacy of Immunodepletion Ex Vivo of Peripheral Blood Progenitor Cell Grafts by Alemtuzumab (Campath in the Bag)

Growth factors mobilize CXCR4 low/negative primitive hematopoietic stem/progenitor cells from the bone marrow of nonhuman primates Nadim Mahmud, Hetal.

Cytomegalovirus-Specific Cytotoxic T Lymphocytes Can Be Efficiently Expanded from Granulocyte Colony-Stimulating Factor–Mobilized Hemopoietic Progenitor.

J. Joseph Melenhorst, Phillip Scheinberg, Ann Williams, David R

Increase of Intermediate Monocytes in Graft-versus-Host Disease: Correlation with MDR1+Th17.1 Levels and the Effect of Prednisolone and 1α,25-Dihydroxyvitamin.

Transplantation of Umbilical Cord Blood–Derived Cells for Novel Indications in Regenerative Therapy or Immune Modulation: A Scoping Review of Clinical.

Natural Killer Cell Differentiation from Hematopoietic Stem Cells: A Comparative Analysis of Heparin- and Stromal Cell–Supported Methods Steven A. Dezell,

Sabina Kersting, Leo F. Verdonck

Caroline F. Morrison, Donna M

B7 Costimulation and Intracellular Indoleamine 2,3-Dioxygenase Expression in Umbilical Cord Blood and Adult Peripheral Blood Enikő Grozdics, László Berta,

Efficacy and Safety of Ex Vivo Cultured Adult Human Mesenchymal Stem Cells (Prochymal™) in Pediatric Patients with Severe Refractory Acute Graft-Versus-Host.

J. J. Nietfeld, Marcelo C. Pasquini, Brent R

Jennifer Crombie, Laura Spring, Shuli Li, Robert J. Soiffer, Joseph H

T Cell–Mediated Rejection of Human CD34+ Cells Is Prevented by Costimulatory Blockade in a Xenograft Model Annie L. Oh, Dolores Mahmud, Benedetta Nicolini,

HCMV Infection of Humanized Mice after Transplantation of G-CSF–Mobilized Peripheral Blood Stem Cells from HCMV-Seropositive Donors Morgan Hakki, Devorah.

Metagenomic Analysis of the Stool Microbiome in Patients Receiving Allogeneic Stem Cell Transplantation: Loss of Diversity Is Associated with Use of Systemic.

A Process to Facilitate Decision Making in Pediatric Stem Cell Transplantation: The Individualized Care Planning and Coordination Model Justin N. Baker,

Clinically Relevant Expansion of Hematopoietic Stem Cells with Conserved Function in a Single-Use, Closed-System Bioprocess Gerard J. Madlambayan, Ian.

Effect of Ex Vivo Culture of CD34+ Bone Marrow Cells on Immune Reconstitution of XSCID Dogs Following Allogeneic Bone Marrow Transplantation Douglas.

Characterization of Cord Blood Natural Killer and Lymphokine Activated Killer Lymphocytes Following Ex Vivo Cellular Engineering Janet Ayello, Carmella.

Atul Sathe, Sterling B. Ortega, Dorothy I. Mundy, Robert H

Chronic Kidney Dysfunction in Patients Alive without Relapse 2 Years after Allogeneic Hematopoietic Stem Cell Transplantation Imad Abboud, Raphaël Porcher,

Laurent Boissel, Hande H

Effect of Total Nucleated and CD34+ Cell Dose on Outcome after Allogeneic Hematopoietic Stem Cell Transplantation Mats Remberger, Johan Törlén, Olle.

Cell-Based Therapy Using Umbilical Cord Blood for Novel Indications in Regenerative Therapy and Immune Modulation: An Updated Systematic Scoping Review.

B Cells and Transplantation: An Educational Resource

Sarah A. Holstein, Paul G. Richardson, Jacob P. Laubach, Philip L

Ravindra Majeti, Christopher Y. Park, Irving L. Weissman

Recovery of Unrelated Donors of Peripheral Blood Stem Cells versus Recovery of Unrelated Donors of Bone Marrow: A Prespecified Analysis from the Phase.

Andy K. W. Hsu, Beverley M. Kerr, Kathryn L. Jones, Richard B

Pharmacokinetic Evaluation of the Drug Interaction between Intravenous Itraconazole and Intravenous Tacrolimus or Intravenous Cyclosporin A in Allogeneic.

Prevalence of Intracellular Galectin-1-Expressing Lymphocytes in Umbilical Cord Blood in Comparison with Adult Peripheral Blood Szonja Kollár, Noémi.

Hematopoietic Engraftment in Recipients of Unrelated Donor Umbilical Cord Blood Is Affected by the CD34+ and CD8+ Cell Doses Seitaro Terakura, Eiichi.

Persistence of host dendritic cells after transplantation is associated with graft-versus- host disease Geoffrey W. Chan, Gullu Gorgun, Kenneth B. Miller,

Protective Immunity Transferred by Infusion of Cytomegalovirus-Specific CD8+ T Cells within Donor Grafts: Its Associations with Cytomegalovirus Reactivation.

Umbilical Cord Blood Xenografts in Immunodeficient Mice Reveal That T Cells Enhance Hematopoietic Engraftment Beyond Overcoming Immune Barriers by Stimulating.

Haploidentical/Mismatched Hematopoietic Stem Cell Transplantation without In Vitro T Cell Depletion for T Cell Acute Lymphoblastic Leukemia Yu Wang,

Steven M. Trifilio, Judy Pi, Jayesh Mehta

Regression Models for Hazard Rates Versus Cumulative Incidence Probabilities in Hematopoietic Cell Transplantation Data Brent R. Logan, Mei-Jie Zhang,

Pauline Damien, David S. Allan

CD25 Blockade Delays Regulatory T Cell Reconstitution and Does Not Prevent Graft- versus-Host Disease After Allogeneic Hematopoietic Cell Transplantation

What is quality in a transplant program?

Volume 13, Issue 1, Pages (January 2006)

Chimeric Antigen Receptor–Modified T Cells: Clinical Translation in Stem Cell Transplantation and Beyond Stanley R. Riddell, Michael C. Jensen, Carl.

Melissa A. Mazur, Craig C. Davis, Paul Szabolcs, MD

Blood and Marrow Transplant Handbook

Varicella-Zoster Virus Disease Is More Frequent after Cord Blood Than after Bone Marrow Transplantation Kristel Vandenbosch, Philippe Ovetchkine, Martin.

Mammen Chandy Biology of Blood and Marrow Transplantation

Prospective Monitoring of Tumor Necrosis Factor α and Interferon γ to Predict the Onset of Acute and Chronic Graft-versus-Host Disease after Allogeneic.

Mary Eapen Biology of Blood and Marrow Transplantation

Futility of Relapsed Diffuse Large B Cell Lymphoma Transplantation?

In Memoriam: E. Donnall Thomas

Optimal Donor Selection: Beyond HLA

Graft Monocytic Myeloid-Derived Suppressor Cell Content Predicts the Risk of Acute Graft-versus-Host Disease after Allogeneic Transplantation of Granulocyte.

Presentation transcript:

Ex vivo expansion of megakaryocyte precursors from umbilical cord blood CD34+ cells in a closed liquid culture system Peter H. Shaw, Devin Gilligan, Xue-Mei Wang, Peter F. Thall, Seth J. Corey Biology of Blood and Marrow Transplantation Volume 9, Issue 3, Pages 151-156 (March 2003) DOI: 10.1016/S1083-8791(03)70003-2 Copyright © 2003 American Society for Blood and Marrow Transplantation Terms and Conditions

Fig. 1 MK lineage expansion in closed liquid culture. Expansion data presented as fold expansion at days 7, 11, and 14 in culture (13 experiments). Note expansion of all subpopulations peaked at day 11. D indicates day. Biology of Blood and Marrow Transplantation 2003 9, 151-156DOI: (10.1016/S1083-8791(03)70003-2) Copyright © 2003 American Society for Blood and Marrow Transplantation Terms and Conditions

Fig. 2 Analysis of CD34 and CD41 expression on a day 11 UCB liquid culture by 3-color flow cytometry. The cells in the upper left quadrant represent cells that have been driven toward megakaryocytic lineage (CD41), the lower right quadrant is the population of expanded, uncommitted (CD34) stem cells, whereas the upper-right quadrant represents the MK precursors (CD34/41 co-expression). Biology of Blood and Marrow Transplantation 2003 9, 151-156DOI: (10.1016/S1083-8791(03)70003-2) Copyright © 2003 American Society for Blood and Marrow Transplantation Terms and Conditions

Fig. 3 Stem cell and MNC expansion in closed liquid culture. Expansion data presented as fold expansion at days 7, 11, and 14 in culture (13 experiments). Note expansion of all subpopulations peaked at day 11 except MNC. D indicates day; MNC, mononuclear cells. Biology of Blood and Marrow Transplantation 2003 9, 151-156DOI: (10.1016/S1083-8791(03)70003-2) Copyright © 2003 American Society for Blood and Marrow Transplantation Terms and Conditions

Fig. 4 Statistical analysis of AC133 expansion variability. The y-axis is fold expansion of the AC133+ population of cells at days 7, 11, and 14. The heavy line indicates the mean, with each box extending from the 25th to the 75th percentile and the whiskers extending to either the most extreme (minimum or maximum) value or 1.5 × the inter-quartile range. Biology of Blood and Marrow Transplantation 2003 9, 151-156DOI: (10.1016/S1083-8791(03)70003-2) Copyright © 2003 American Society for Blood and Marrow Transplantation Terms and Conditions