Prevention of seizures and reorganization of hippocampal functions by transplantation of bone marrow cells in the acute phase of experimental epilepsy

Slides:

Advertisements

Similar presentations

Fig. 3. Expression of Ninj1protein in cortices of the ischemic hemispheres of the rat brain at 4 day post-MCAO. Coronal brain sections were prepared at.

Advertisements

Activation of Src Family Kinases in Spinal Microglia Contributes to Formalin-Induced Persistent Pain State Through p38 Pathway Yong-Hui Tan, Kai Li,

Postischemic administration of liposome-encapsulated luteolin prevents against ischemia-reperfusion injury in a rat middle cerebral artery occlusion model

Volume 115, Pages (September 2015)

Seizure - European Journal of Epilepsy

Ian Morrison, Saif S.M. Razvi Seizure - European Journal of Epilepsy

Experimental Neonatal Status Epilepticus and the Development of Temporal Lobe Epilepsy with Unilateral Hippocampal Sclerosis Mark Dunleavy, Sachiko Shinoda,

Anne A. Kan, W. Saskia van der Hel, Sharon M. Kolk, Ineke W. M

Fig. 2. Expression of Ninj1 in cortices of ischemic hemispheres of rat brains at 1 day post-MCAO. Coronal brain sections were prepared at 1 day post-MCAO.

Okadaic-Acid-Induced Inhibition of Protein Phosphatase 2A Produces Activation of Mitogen-Activated Protein Kinases ERK1/2, MEK1/2, and p70 S6, Similar.

Volume 86, Issue 5, Pages (June 2015)

Volume 48, Issue 5, Pages (December 2005)

Motor Protein KIF1A Is Essential for Hippocampal Synaptogenesis and Learning Enhancement in an Enriched Environment Makoto Kondo, Yosuke Takei, Nobutaka.

Johanna Sigl-Glöckner, Michael Brecht Cell Reports

Oxidative stress mediates hippocampal neuron death in rats after lithium–pilocarpine- induced status epilepticus Jinzhi Liu, Aihua Wang, Lili Li, Yanfei.

Isabella Maiellaro, Martin J. Lohse, Robert J. Kittel, Davide Calebiro

Volume 14, Issue 8, Pages (March 2016)

TLR4, ATF-3 and IL8 inflammation mediator expression correlates with seizure frequency in human epileptic brain tissue Katharina Pernhorst, Stefan Herms,

Fluctuating and constant valproate administration gives equivalent seizure control in rats with genetic and acquired epilepsy S. Dedeurwaerdere, L. van.

Volume 44, Issue 3, Pages (October 2004)

The anti-ictogenic effects of levetiracetam are mirrored by interictal spiking and high- frequency oscillation changes in a model of temporal lobe epilepsy

Alternative technique or mitigating strategy for sevoflurane-induced neurodegeneration: a randomized controlled dose-escalation study of dexmedetomidine.

Brain regional vulnerability to anaesthesia-induced neuroapoptosis shifts with age at exposure and extends into adulthood for some regions M. Deng, R.D.

Volume 139, Issue 4, Pages (November 2009)

Benjamin Cretin, Nathalie Philippi

Volume 15, Issue 9, Pages (May 2016)

Volume 15, Issue 7, Pages (May 2016)

Calcium Ion Gradients and Dynamics in Cultured Skin Slices of Rat Hindpaw in Response to Stimulation with ATP Moe Tsutsumi, Sumiko Denda, Kaori Inoue,

Volume 15, Issue 12, Pages (June 2005)

Volume 23, Issue 5, Pages (May 2018)

Interferon-γ enhances the efficacy of autogenous bone grafts by inhibiting postoperative bone resorption in rat calvarial defects Peiqi Li, DDS, Yoshitomo.

Molecular Therapy - Methods & Clinical Development

Volume 11, Issue 12, Pages (June 2015)

Volume 89, Issue 5, Pages (March 2016)

Recruitment of N-Type Ca2+ Channels during LTP Enhances Low Release Efficacy of Hippocampal CA1 Perforant Path Synapses Mohsin S. Ahmed, Steven A. Siegelbaum

Volume 89, Issue 5, Pages (March 2016)

Volume 110, Issue 4, Pages (August 2002)

High Plasticity of New Granule Cells in the Aging Hippocampus

Seizures in the Developing Brain

Johanna Sigl-Glöckner, Michael Brecht Cell Reports

Single sevoflurane exposure decreases neuronal nitric oxide synthase levels in the hippocampus of developing rats X. Feng, J.J. Liu, X. Zhou, F.H. Song,

Volume 88, Issue 3, Pages (November 2015)

Volume 99, Issue 12, Pages (December 2010)

Nicotine inhibits hippocampal and striatal acetylcholinesterase activities, and demonstrates dual action on adult neuronal proliferation and maturation

Volume 43, Issue 1, Pages (July 2004)

Volume 89, Issue 5, Pages (March 2016)

Zhenglin Gu, Jerrel L. Yakel Neuron

Impaired Dendritic Expression and Plasticity of h-Channels in the fmr1−/y Mouse Model of Fragile X Syndrome Darrin H. Brager, Arvin R. Akhavan, Daniel.

Volume 18, Issue 10, Pages (March 2017)

Volume 3, Issue 3, Pages (September 2014)

High Plasticity of New Granule Cells in the Aging Hippocampus

Volume 53, Issue 5, Pages (March 2007)

DFP-induced astrogliosis is reduced by urethane.

Evidence for an Age-Dependent Decline in Axon Regeneration in the Adult Mammalian Central Nervous System Cédric G. Geoffroy, Brett J. Hilton, Wolfram.

Functional MRI Evidence for LTP-Induced Neural Network Reorganization

Volume 26, Issue 7, Pages e4 (February 2019)

Volume 11, Issue 11, Pages (June 2015)

Volume 17, Issue 2, Pages (October 2016)

Volume 2, Issue 3, Pages (March 2014)

Volume 49, Issue 4, Pages (February 2006)

Volume 23, Issue 4, Pages (August 1999)

Fig. 4. Reduction of apoptosis and autophagic stress by treatment with silibinin in the KA-treated hippocampus. (A, B) Double-immunofluorescence staining.

P.J. Moss, W. Huang, J. Dawes, K. Okuse, S.B. McMahon, A.S.C. Rice

Fig. 3. Neuroprotective effects of silibinin against KA-induced excitotoxicity. (A) Silibinin was intraperitoneally injected for 8 days, starting 1 day.

Fig. 5. Anti-inflammatory effects of silibinin on KA treatment in the hippocampus. (A) Representative coronal sections of the hippocampal CA1 region following.

Xenon improves long-term cognitive function, reduces neuronal loss and chronic neuroinflammation, and improves survival after traumatic brain injury in.

PTZ-induced neuronal activity visualized by IEGs

Volume 28, Issue 5, Pages e5 (July 2019)

Irreplaceability of Neuronal Ensembles after Memory Allocation

Volume 27, Issue 10, Pages e5 (June 2019)

Presentation transcript:

Prevention of seizures and reorganization of hippocampal functions by transplantation of bone marrow cells in the acute phase of experimental epilepsy Zaquer S.M. Costa-Ferro, Affonso S. Vitola, Michele F. Pedroso, Fernanda B. Cunha, Léder L. Xavier, Denise C. Machado, Milena B.P. Soares, Ricardo Ribeiro-dos-Santos, Jaderson C. daCosta Seizure - European Journal of Epilepsy Volume 19, Issue 2, Pages 84-92 (March 2010) DOI: 10.1016/j.seizure.2009.12.003 Copyright © 2010 Terms and Conditions

Fig. 1 Electrophysiological properties of brain sections of normal and epileptic rats. Hippocampal slices were prepared from, saline-epileptic, BMC-epileptic and non-epileptic controls at 10 and 120 days after SE. (A) Insets are superimposed representative traces of the averaged fEPSPs before (−20 to −15min; gray line) and after (44–48min; dark line) tetanic stimulation in non-epileptic control (a and d), in saline-epileptic (b and e), and BMC-epileptic (c and f) groups. Normalized amplitudes of fEPSPs in non-epileptic control (dark squares), saline-epileptic (open triangles), and BMC-epileptic (open circles) groups killed 10 (B) and 120 (C) days after SE (n=9–10 slices/group). Data are presented as mean±S.E.M. Seizure - European Journal of Epilepsy 2010 19, 84-92DOI: (10.1016/j.seizure.2009.12.003) Copyright © 2010 Terms and Conditions

Fig. 2 Effect treatment with bone marrow in the hippocampal cells. Nissl-stained sections showing hippocampal structure [A=in a coronal section of non-epileptic rats, B=CA1; D=CA3; F=dentate gyrus] of epileptic rats and (B′, D′, F′; CA1, CA3 and dentate gyrus, respectively) from BMC-treated rats during epileptogenesis (10 days after transplantation). In the chronic phase (120 days after transplantation) (C=CA1; E=CA3, G=dentate gyrus) of epileptic rats and BMC-treated rats (C′, E′, G′, CA1, CA3 and dentate gyrus, respectively). Scale bar=100μm. Seizure - European Journal of Epilepsy 2010 19, 84-92DOI: (10.1016/j.seizure.2009.12.003) Copyright © 2010 Terms and Conditions

Fig. 3 (A–C) Effect of Li-pilo-induced epilepsy and treatment with bone marrow on cells on neuronal density. Comparisons of neuronal density per cubic millimeter volume of tissue among different hippocampal sections were performed during epileptogenesis (10 days after Li-Pilo) and/or during the chronic phase (120 days after Li-Pilo) in BMC-epileptic, saline-epileptic and non-epileptic controls (2 hemispheres of 5 rats, per group). The groups were compared by one-way ANOVA followed by Tukey post hoc test. *p<0.05; **p<0.01; ***p<0.001 indicate significant differences compared to baseline; symbol (a) indicates a significant difference between the BMC-epilepsy and saline-epilepsy groups. AOI=area of interest (800μm2). Data are reported as mean±S.E.M. Seizure - European Journal of Epilepsy 2010 19, 84-92DOI: (10.1016/j.seizure.2009.12.003) Copyright © 2010 Terms and Conditions

Fig. 4 Effects of BMC treatment on hippocampal volume in Li-pilo-induced epilepsy. The hippocampal volume analyzed (24.000μm3) was calculated from 5 rats in each group (non-epileptic control, saline-epileptic, and BMC-epileptic) killed 10 and 120 days after SE. The groups were compared by one-way ANOVA followed by Tukey post hoc test. **p<0.01; ***p<0.001. Results are expressed as mean±S.D. Seizure - European Journal of Epilepsy 2010 19, 84-92DOI: (10.1016/j.seizure.2009.12.003) Copyright © 2010 Terms and Conditions

Fig. 5 Presence of donor-derived cells in brains of epileptic rats. Photomicrographs of coronal sections obtained 24h after transplantation, stained for visualization of NeuN+ (red) and GFP+ (green) cells in ectorhinal cortex (A), and hilus of GD (B). Section of the stratum radiatum of the hippocampus (C) obtained 48h after transplantation showing GFP+ cells (green). GFP+ cells (green) shown in sections of the CA1 region (E) and hilus (F) 3 and 5 days after transplantation, respectively. Section of the plexus 10 days after transplantation showing cells expressing microglia markers (F) and the (G) amygdalostriatal transition area showed GFAP+ (red) and GFP+ (green) cells. (H) Section of perirhinal cortex 120 days after transplantation showing GFP+ cells (green). DAPI-stained nuclei are shown in blue. Images (A)–(C) and (G) and (H) were obtained by confocal microscopy, and the micrographs (D–F) by conventional florescence microscopy. Arrows indicate GFP+ cells. Bar=100μm. Seizure - European Journal of Epilepsy 2010 19, 84-92DOI: (10.1016/j.seizure.2009.12.003) Copyright © 2010 Terms and Conditions