Jeung-Yeal Ahn, Katie Seo, Olga Weinberg, Scott D. Boyd, Daniel A

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Presentation transcript:

A Comparison of Two Methods for Screening CEBPA Mutations in Patients with Acute Myeloid Leukemia Jeung-Yeal Ahn, Katie Seo, Olga Weinberg, Scott D. Boyd, Daniel A. Arber The Journal of Molecular Diagnostics Volume 11, Issue 4, Pages 319-323 (July 2009) DOI: 10.2353/jmoldx.2009.080121 Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Schematic representation of the location of multiplex-PCR fragment and direct sequencing primers on CEBPA. The Journal of Molecular Diagnostics 2009 11, 319-323DOI: (10.2353/jmoldx.2009.080121) Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Schematic representation of the ten CEBPA mutations detected in the current study. The Journal of Molecular Diagnostics 2009 11, 319-323DOI: (10.2353/jmoldx.2009.080121) Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 Gene scan electropherogram from multiplex PCR method and partial sequence of CEBPA from sequencing method (numbering according to GenBank access number U34070). A: Detected mutation (G9: nt 1528 to 1529 ins AGA) by sequencing and fragment analysis methods. B: Detected mutation (A027: nt 787 sub GCCT>CTAC) by sequencing method alone. * W: wild (normal) peak, M: mutation peak. The Journal of Molecular Diagnostics 2009 11, 319-323DOI: (10.2353/jmoldx.2009.080121) Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions