Gene Sizes Vary Strachan p146 DYSTROPHIN.

Slides:



Advertisements
Similar presentations
Outcome: Targeted exon is skipped or not Question answered:Can the oligo induce exon skipping? Summary: Use of oligonucleotides to change splicing Tool.
Advertisements

LECTURE 17: RNA TRANSCRIPTION, PROCESSING, TURNOVER Levels of specific messenger RNAs can differ in different types of cells and at different times in.
Regulation of eukaryotic gene sequence expression Lecture 6.
Eukaryotic Transcription
Xiaole Shirley Liu STAT115, STAT215, BIO298, BIST520
Tutorial 7 Genome browser. Free, open source, on-line broswer for genomes Contains ~100 genomes, from nematodes to human. Many tools that can be used.
BME 130 – Genomes Lecture 7 Genome Annotation I – Gene finding & function predictions.
Eukaryotic Gene Finding
CHAPTER 3 GENE EXPRESSION IN EUKARYOTES (cont.) MISS NUR SHALENA SOFIAN.
 ribose  Adenine  Uracil  Adenine  Single.
Processing of eukaryotic pre-mRNA Human dystrophin gene has 79 exons, spans over 2,300-Kb and requires over 16 hours to be transcribed! For primary transcripts.
Chapter 6 Gene Prediction: Finding Genes in the Human Genome.
RNA Editing Definition: any process, other than splicing, that results in a change in the sequence of a RNA transcript such that it differs from the sequence.
Post-transcriptional Processing. Processing Events in Prokaryotes vs Eukaryotes.
Gene Activity: How Genes Work
Protein Synthesis. DNA acts like an "instruction manual“ – it provides all the information needed to function the actual work of translating the information.
1. Bacterial genomes - genes tightly packed, no introns... HOW TO FIND GENES WITHIN A DNA SEQUENCE? Scan for ORFs (open reading frames) - check all 6 reading.
1 The Interrupted Gene. Ex Biochem c3-interrupted gene Introduction Figure 3.1.
Expression of the Genome The transcriptome. Decoding the Genetic Information  Information encoded in nucleotide sequences contained in discrete units.
ModENCODE August 20-21, 2007 Drosophila Transcriptome: Aim 2.2.
Chapter 14 – RNA molecules and RNA processing
Fig.1.8 DNA STRUCTURE 5’ 3’ Antiparallel DNA strands Hydrogen bonds between bases DOUBLE HELIX 5’ 3’
P ROTEIN SYNTHESIS. The base sequence of DNA codes for the amino acids that make up a protein (one gene codes for one polypeptide).
BioSci 203 blumberg lecture 7 page 1 © copyright Bruce Blumberg All rights reserved Bio Sci 203 bb lecture 7 - mRNA analysis Bruce Blumberg.
Chapters 26 Lehninger 5th Edition
Mark D. Adams Dept. of Genetics 9/10/04
GenePolypeptide Gene  Polypeptide Transcription 1.RNAP binds to promoter 2.Separates DNA strands 3.Transcribes the DNA (adds RNA nucleotides in a 5'-3'
Eukaryotic Gene Structure. 2 Terminology Genome – entire genetic material of an individual Transcriptome – set of transcribed sequences Proteome – set.
Genome-wide association study between DSE polymorphism and Poly-A usage in Human population Hiren Karathia Sridhar Hannenhalli.
While replication, one strand will form a continuous copy while the other form a series of short “Okazaki” fragments Genetic traits can be transferred.
Chapter 3 The Interrupted Gene.
Transcriptome What is it - genome wide transcript abundance How do you obtain it - Arrays + MPSS What do you do with it when you have it - ?
Objectives Genome-wide investigation – to estimate alternate Poly-Adenylation (APA) usage on 3’UTR – to identify polymorphism of Downstream Sequence Elements.
Alternative Splicing. mRNA Splicing During RNA processing internal segments are removed from the transcript and the remaining segments spliced together.
1 Gene Finding. 2 “The Central Dogma” TranscriptionTranslation RNA Protein.
EGASP 2005 Evaluation Protocol
Eukaryotic Gene Structure
This paper is about RNA can inhibit gene expression
EGASP 2005 Evaluation Protocol
Experimental Verification Department of Genetic Medicine
Expression of the Genome
Key points: summarized from chapter 14
Transcription.
Chapter 4 “DNA Finger Printing”
Molecular Therapy - Nucleic Acids
Small RNA and Cyanobacteria
Protein Synthesis Genetics.
Key points: summarized from chapter 14
Recitation 7 2/4/09 PSSMs+Gene finding
COX-1 and known variants.
Genome Editing with Apollo
Transcription.
Chapter 4 The Interrupted Gene.
Allan Award Lecture: On Jumping Fields and “Jumping Genes”
A PASport to Cellular Proliferation
Key points: summarized from chapter 14
Analysis of an exon 1 polymorphism of the B2 bradykinin receptor gene and its transcript in normal subjects and patients with C1 inhibitor deficiency 
Volume 18, Issue 2, Pages (April 2005)
Gene Sizes Vary Strachan p146 DYSTROPHIN.
GT repeats are unique to Cdk6 and are conserved in different mammals.
(and some other important stuff)
Emmanuelle Bitoun, Stéphane Chavanas, Alan D
Gene Structure.
Barc knockdown causes intron retention of short, GC-rich introns with weak splice sites. barc knockdown causes intron retention of short, GC-rich introns.
Multiple-exon skipping.
Structure of mdm2 gene and protein
Genomic structure of LTBP-4 around the 3rd 8-Cys repeat.
Sharon Si Jia Ng, Ruther Teo Zheng, Ismail Osman, Jun Wei Pek
Allan Award Lecture: On Jumping Fields and “Jumping Genes”
Gene Structure.
Presentation transcript:

Gene Sizes Vary Strachan p146 DYSTROPHIN

Multiple Small Exons are Typical Strachan p191

Full-length first strand cDNA

Normalize

Normalize

Alternative Splicing Strachan p187

5’ RACE Miesfeld p153

Miesfeld p163 cont.

Short Splicing Signals Brown (Genomes) p213

Finding Splice Sites C T G G T A A G T Exon Intron

100nt INTRON 1st EXON 1.5kb 800nt 5kb 150nt 150nt Last EXON Mid EXON 800nt

Short Polyadenylation signals Brown (Genomes) 212

RNA Editing

MicroRNAs

Northern RNA blot Strachan p. 110

TaqMan Strachan p129

OLIGOS cDNAs

Sensitivity

RNASeq

Feedback: Privacy Policy Feedback
Do Not Sell My Personal Information
About project: SlidePlayer Terms of Service

© 2025 SlidePlayer.com. Inc. All rights reserved.