MicroRNAs of rat articular cartilage at different developmental stages identified by Solexa sequencing J. Sun, N. Zhong, Q. Li, Z. Min, W. Zhao, Q. Sun,

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Presentation transcript:

MicroRNAs of rat articular cartilage at different developmental stages identified by Solexa sequencing J. Sun, N. Zhong, Q. Li, Z. Min, W. Zhao, Q. Sun, L. Tian, H. Yu, Q. Shi, F. Zhang, S. Lu Osteoarthritis and Cartilage Volume 19, Issue 10, Pages 1237-1245 (October 2011) DOI: 10.1016/j.joca.2011.07.002 Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Histology of left femoral head at different development stages and expression of cartilage-specific genes. Representative images of the left femoral heads collected from rats at postnatal day 0 (A), day 21(B) and day 42 (C) and stained with HE. The purple arrows indicate the epiphyseal plate and the black arrows indicate dividing line from which cartilage is removed for the experiment. The expression levels of the Sox9 (D), aggrecan (E), ColIIα1 (F) and ColXα1 (G) were evaluated by RT-qPCR. * and ** represent P<0.05 and P<0.01, respectively, between two groups (n=5). Osteoarthritis and Cartilage 2011 19, 1237-1245DOI: (10.1016/j.joca.2011.07.002) Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Expression fold changes of miRNA gene among three libraries. After normalization to 1TPM, each known miRNA gene from one library was compared to the corresponding one from another by log2 ratio fold change (A, B, C). The detail information of all 310 known miRNAs and miRNAs* detected in this study is available in Supplemental Table S4. Osteoarthritis and Cartilage 2011 19, 1237-1245DOI: (10.1016/j.joca.2011.07.002) Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Schematic diagram of differentially expressed miRNA roles in regulating chondrocyte proliferation and differentiation at different developmental stages predicted by bioinformatical analyses. Plus signs indicate to stimulate or activate pathways and minus signs indicate to inhibit or suppress pathways. The fold change of the differential expression of all the predicted miRNAs is more than two. PTHrP: parathyroid hormone-related protein; Sox9. Osteoarthritis and Cartilage 2011 19, 1237-1245DOI: (10.1016/j.joca.2011.07.002) Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 The expression of six miRNAs at different developmental stages of articular cartilage detected by Solexa sequencing and RT-qPCR. The expression levels of the indicated miRNAs in rat were evaluated by stem-loop RT-qPCR with 40 cycles. Two confirmed miRNAs (rno-miR-140 (A) and rno-miR-26a (B)) and four unverified miRNAs (rno-miR-25 (C), rno-miR-181a (D), rno-miR-1 (E) and rno-miR-150 (F)) were random selected. The left panel in each miRNA is the expression determined by RT-qPCR (n=5) and the right panel is cloning reading obtained from Solexa sequencing. Osteoarthritis and Cartilage 2011 19, 1237-1245DOI: (10.1016/j.joca.2011.07.002) Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions