Cytoplasmic, rather than nuclear-DNA, insufficiencies as the major cause of poor competence of vitrified oocytes  Sayyed Morteza Hosseini, Vajihe Asgari, M. Hajian, Mohammad Hossein Nasr-Esfahani  Reproductive BioMedicine Online  Volume 30, Issue 5, Pages 549-552 (May 2015) DOI: 10.1016/j.rbmo.2015.01.003 Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Differential contributions of karyoplast and cytoplast to the developmental competence of vitrified oocytes. Vitrifed in-vitro matured oocytes and their fresh siblings were enucleated to prepare vitrified- karyoplast (VK) and cytoplasts (VC) and fresh- karyoplast (FK) and cytoplast (FC). These were used for reconstitution of four types of oocytes (FK/FC, VK/VC, FK/VC and VK/FC) that were used then for assessment of embryo development. a–cValues with different letters are significantly different at P ≤ 0.05. Reproductive BioMedicine Online 2015 30, 549-552DOI: (10.1016/j.rbmo.2015.01.003) Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions