Supplemental materials

Slides:

Advertisements

Similar presentations

PGE 2 (pg/ml) a b Procollagen I serum aspirin (400  M) h GAPDH Figure S1. Serum-up-regulated PGE 2 suppresses procollagen I protein expression.

Advertisements

Volume 56, Issue 5, Pages (November 1999)

Volume 48, Issue 6, Pages (June 2011)

IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,

From: Regulation of Cdc42 Expression and Signaling Is Critical for Promoting Corneal Epithelial Wound Healing Invest. Ophthalmol. Vis. Sci ;54(8):

Volume 68, Issue 4, Pages (October 2005)

Vascular Endothelial–Cadherin Tyrosine Phosphorylation in Angiogenic and Quiescent Adult Tissues by Nathalie Lambeng, Yann Wallez, Christine Rampon, Francine.

Volume 60, Issue 2, Pages (August 2001)

CFTR stabilizes ENaC at the plasma membrane

Volume 66, Issue 4, Pages (October 2004)

The cartilage chondrolytic mechanism of fibronectin fragments involves MAP kinases: comparison of three fragments and native fibronectin L. Ding, Ph.D.,

Constitutive Phosphorylation of Focal Adhesion Kinase Is Involved in the Myofibroblast Differentiation of Scleroderma Fibroblasts Yoshihiro Mimura, Hironobu.

Shitao Li, Lingyan Wang, Michael A. Berman, Ye Zhang, Martin E. Dorf

Volume 75, Issue 11, Pages (June 2009)

Volume 13, Issue 4, Pages (February 2003)

Autocrine and paracrine functions of vascular endothelial growth factor (VEGF) in renal tubular epithelial cells Guillermo Villegas, Bäerbel Lange-Sperandio,

C. Jacques, Ph. D. , A. D. Recklies, Ph. D. , A. Levy, F. Berenbaum, M

Hyaluronan promotes the chondrocyte response to BMP-7

Naoko Kanda, Shinichi Watanabe Journal of Investigative Dermatology

by Xingwei Sui, Sanford B. Krantz, Min You, and Zhizhuang Zhao

Differential regulation of cytokine-induced MMP-1 and MMP-13 expression by p38 kinase inhibitors in human chondrosarcoma cells: potential role of Runx2.

Human osteoarthritic chondrocytes are impaired in matrix metalloproteinase-13 inhibition by IFN-γ due to reduced IFN-γ receptor levels R. Ahmad, M. El.

Volume 22, Issue 5, Pages (May 2012)

Translocation of protein kinase C isoforms is involved in propofol-induced endothelial nitric oxide synthase activation L. Wang, B. Wu, Y. Sun, T. Xu,

Volume 56, Issue 5, Pages (November 1999)

Volume 13, Issue 9, Pages (April 2003)

Pnut is phosphorylated during early Drosophila embryogenesis.

FAS1 domain protein inhibits association of αvβ3 integrin with VEGFR-2 and attenuates VEGF165-induced VEGFR-2 phosphorylation. FAS1 domain protein inhibits.

Volume 15, Issue 7, Pages (May 2016)

Lipotransin Molecular Cell

A C D B Asynchronous nM Nocodazole 150 ► 100 ► 75 ►

Volume 68, Issue 4, Pages (October 2005)

Gangliosides GD1b, GT1b, and GQ1b Suppress the Growth of Human Melanoma by Inhibiting Interleukin-8 Production: the Inhibition of Adenylate Cyclase1

Arachidonic acid induces ERK activation via Src SH2 domain association with the epidermal growth factor receptor L.D. Alexander, Y. Ding, S. Alagarsamy,

AKT Status Controls Susceptibility of Malignant Keratinocytes to the Early-Activated and UVB-Induced Apoptotic Pathway David Decraene, An Van Laethem,

Hyaluronan increases glomerular cyclooxygenase-2 protein expression in a p38 MAP- kinase–dependent process Marjorie E. Dunlop, Ph.D., Evelyne E. Muggli

Histamine Inhibits the Production of Interferon-induced Protein of 10 kDa in Human Squamous Cell Carcinoma and Melanoma Naoko Kanda, Shinichi Watanabe

Inhibition of bile salt-induced apoptosis by cyclic AMP involves serine/threonine phosphorylation of CD95 Roland Reinehr, Dieter Häussinger Gastroenterology

DNA binding of activator protein-1 is increased in human mesangial cells cultured in high glucose concentrations William A. Wilmer, Fernando G. Cosio

Interleukin-6-Resistant Melanoma Cells Exhibit Reduced Activation of STAT3 and Lack of Inhibition of Cyclin E-Associated Kinase Activity Markus Böhm,

Chi-Hyun Park, Youngji Moon, Chung Min Shin, Jin Ho Chung

Terunao Takahara, Tatsuya Maeda Molecular Cell

Human Keratinocytes Respond to Osmotic Stress by p38 Map Kinase Regulated Induction of HSP70 and HSP27 M. Garmyn, A. Pupe Journal of Investigative Dermatology

Heat Shock-Induced Matrix Metalloproteinase (MMP)-1 and MMP-3 Are Mediated through ERK and JNK Activation and via an Autocrine Interleukin-6 Loop Chi-Hyun.

IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,

Rsk1 mediates a MEK–MAP kinase cell survival signal

17β-Estradiol Inhibits Oxidative Stress-Induced Apoptosis in Keratinocytes by Promoting Bcl-2 Expression Naoko Kanda, Shinichi Watanabe Journal of Investigative.

Small heat shock protein alteration provides a mechanism to reduce mesangial cell contractility in diabetes and oxidative stress Marjorie E. Dunlop,

pY-Tie2 (IP with anti-Tie2) Tie2 P-RET RET P-KIT KIT GAPDH P-AXL AXL

Volume 60, Issue 3, Pages (September 2001)

Yvonne Ng, Georg Ramm, Jamie A. Lopez, David E. James Cell Metabolism

Prolonged Rapamycin Treatment Inhibits mTORC2 Assembly and Akt/PKB

Modulation of renal epithelial barrier function by mitogen-activated protein kinases (MAPKs): Mechanism of cyclosporine A–induced increase in transepithelial.

The effects of laminarin, PMA and zymosan on PKC phosphorylation.

Time-dependent phosphorylation profile of p38-MAPK and JNKs in samples from gills of Mytilus galloprovincialis specimens subjected to hyperthermia (30°C).

Growth Factor-Dependent Trafficking of Cerebellar NMDA Receptors via Protein Kinase B/Akt Phosphorylation of NR2C Bo-Shiun Chen, Katherine W. Roche

Fibronectin-induced integrin-mediated PDGFR-β tyrosine phosphorylation

Phosphorylation of AMPK-Thr172 before and after metformin treatment in muscle from subjects with type 2 diabetes. Phosphorylation of AMPK-Thr172 before.

CML-HSA treatment activates MAPK family members ERK1/2 and p38 but not JNK. Samples were taken at the indicated times after 100 μg/ml CML-HSA exposure.

Phosphorylation of CBP by IKKα Promotes Cell Growth by Switching the Binding Preference of CBP from p53 to NF-κB Wei-Chien Huang, Tsai-Kai Ju, Mien-Chie.

Induction of CDCP1 is regulated by Ras in NSCLC cells.

Volume 19, Issue 1, Pages (July 1997)

Volume 22, Issue 3, Pages (May 2006)

Phosphorylation of the pro-apoptotic protein BAD on serine 155, a novel site, contributes to cell survival K. Virdee, P.A. Parone, A.M. Tolkovsky Current.

Expression of dominant-negative RasN17 completely suppresses Ras activation in Rh1 cells. Expression of dominant-negative RasN17 completely suppresses.

Volume 12, Issue 6, Pages (March 2002)

Induction of PARP cleavage (A) and activation of caspases (B) after treatment with a combination of TRAIL and cisplatin. Induction of PARP cleavage (A)

A Novel Role for FAK as a Protease-Targeting Adaptor Protein

Expression and induction of HER2 and HPSE in 231BMBC cells.

Protein Phosphorylation and Prevention of Cytochrome Oxidase Inhibition by ATP: Coupled Mechanisms of Energy Metabolism Regulation Rebeca Acin-Perez,

Presentation transcript:

Supplemental materials * * Phospho-PKA * * * * * no treatment 10 M H89 3 M KT 5720 50 M Rp- cAMPS 10 M FK Figure S1.　Effects of PKA inhibitors on phosphorylation state of multiple proteins in hRPE1 cells. In order to evaluate the impact of PKA inhibitors, we used a phospho-PKA substrate antibody to detect the global pattern of phosphorylation state in hRPE1 cells. The cells were grown to ~90% confluency in serum-containing medium, followed by serum starvation for 24 h. Subsequently, the cells were cultured with the indicated drugs for 3 h (H89, KT5720, and Rp-cAMPS) or 30 min (forskolin: FK). Proteins in the cell lysates were separated by SDS-PAGE and analyzed by immunoblotting with an anti-phospho-(Ser/Thr) PKA substrate-selective [K/R][K/R]X[S/T] antibody. The membranes shown are representative results from three independent experiments. Several bands show a less phosphorylated state () in response to individual PKA inhibitor compared to control, which corresponds to increase PKA activity by treatment of forskolin (FK, arrowhead). Each PKA inhibitor did not completely diminish all phosphor-PKA substrates, indicating that phosphorylation state of multiple protein may be controlled by some additional mechanism. Further, individual PKA inhibitor caused a different spectrum for diminishing phosphorylation state. It might be speculated that each PKA inhibitor is responsible for a different functionality of PKA holoenzyme in addition to non-PKA-based effects [37].