2. Electron Microscopy.

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Presentation transcript:

2. Electron Microscopy

A. Transmission electron Microscopy (TEM)

LM vs. TEM

TEM Fixatives:

3-D reconstruction of a mitochondrion from TEM sections

Immunogold localization (TEM)

Replica technique for surfaces

TEM of replica of freeze-fractured chloroplast

TEM of negatively stained actin filaments (Uranium acetate)

B. Scanning Electron Microscopy

SEM DIC TEM

Radioisotope labeling

Radioisotopically labeled molecules

EM Autoradiography Labeled Pancreatic beta Insulin: cells pulsed w 3H-leucine for 10’ 5 min, followed grains over by 10’ and 45’ Golgi app. chase. Sections coated w photo emulsion and 45’ exposed in dark. Grains over Grains secretory developed and granuled photographed in TEM