Figure 2 Cas9 targeting using crRNA (CRISPR

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Figure 2 Cas9 targeting using crRNA (CRISPR RNA)–tracrRNA (trans activating crRNA) or a single guide RNA chimera Figure 2 | Cas9 targeting using crRNA (CRISPR RNA)–tracrRNA (trans activating crRNA) or a single guide RNA chimera. a | In type II CRISPR/Cas9 systems, Cas9 is guided by a two-RNA structure formed by tracrRNA and crRNA to cleave targeted double-stranded DNA (dsDNA). b | A chimeric RNA generated by fusing crRNA to tracrRNA via a linker loop is able to target and cleave dsDNA. Permission obtained from Science © Jinek, M. et al. Science 337, 816–821 (2012). Nt, nucelotide Permission obtained from Science © Jinek, M. et al. Science 337, 816–821 (2012). Gibson, G. J. & Yang, M. (2017) What rheumatologists need to know about CRISPR/Cas9 Nat. Rev. Rheumatol. doi:10.1038/nrrheum.2017.6