COL1A1-PDGFB Fusion Transcripts in Fibrosarcomatous Areas of Six Dermatofibrosarcomas Protuberans Jian Wang, Yosuke Morimitsu, Sumika Okamoto, Masanori.

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COL1A1-PDGFB Fusion Transcripts in Fibrosarcomatous Areas of Six Dermatofibrosarcomas Protuberans Jian Wang, Yosuke Morimitsu, Sumika Okamoto, Masanori Hisaoka, Tsuyoshi Ishida, Weiqi Sheng, Hiroshi Hashimoto The Journal of Molecular Diagnostics Volume 2, Issue 1, Pages 47-52 (February 2000) DOI: 10.1016/S1525-1578(10)60614-9 Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Photomicrographs of dermatofibrosarcoma protuberans with fibrosarcomatous areas before (A) and after microdissection (B) in case 1.Asterisk represents a fibrosarcomatous area;triangle represents a conventional DFSP area (H&E; original magnification, ×2.5). The Journal of Molecular Diagnostics 2000 2, 47-52DOI: (10.1016/S1525-1578(10)60614-9) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Histological appearance of DFSP-FS. A: Distinct storiform pattern in a conventional DFSP area with characteristic honeycomb infiltration of the adjacent fat tissue (case 4; H&E; original magnification, ×25).B: Herringbone structure in a fibrosarcomatous area of DFSP-FS (case 4; H&E; original magnification, ×50). The Journal of Molecular Diagnostics 2000 2, 47-52DOI: (10.1016/S1525-1578(10)60614-9) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 Expression of COL1A1-PDGFB fusion transcripts (A) and PBGD gene mRNA (housekeeping gene;B) in each sample of DFSP-FS. The number of the lane is identical to the case number in Table 2. The double bands in lane 5D were likely to be formed by DNA conformation changes, because the DNA sequences of the PCR products in both upper and lower bands in lane 5D were confirmed to be identical by the sequence analysis. M, 100-bp DNA ladder; D, conventional DFSP areas; F, fibrosarcomatous areas; N, negative control. The Journal of Molecular Diagnostics 2000 2, 47-52DOI: (10.1016/S1525-1578(10)60614-9) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 4 Nucleotide sequence analysis of PCR products showing three types of COL1A1-PDGFB fusion transcripts composed of junctions between PDGFB exon 2 and COL1A1 exon 25 (A), exon 32 (B), or exon 46 (C). The Journal of Molecular Diagnostics 2000 2, 47-52DOI: (10.1016/S1525-1578(10)60614-9) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions